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原发性干燥综合征患者唾液腺组织ceRNA调控网络的构建

吴玲玲, 凌华云, 周颖, 邱丽娟, 王红, 薛昱, 陈慧娟, 王廷睿, 王斌. 原发性干燥综合征患者唾液腺组织ceRNA调控网络的构建[J]. 中华疾病控制杂志, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006
引用本文: 吴玲玲, 凌华云, 周颖, 邱丽娟, 王红, 薛昱, 陈慧娟, 王廷睿, 王斌. 原发性干燥综合征患者唾液腺组织ceRNA调控网络的构建[J]. 中华疾病控制杂志, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006
WU Ling-ling, LING Hua-yun, ZHOU Ying, QIU Li-juan, WANG Hong, XUE Yu, CHEN Hui-juan, WANG Ting-rui, WANG Bin. Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006
Citation: WU Ling-ling, LING Hua-yun, ZHOU Ying, QIU Li-juan, WANG Hong, XUE Yu, CHEN Hui-juan, WANG Ting-rui, WANG Bin. Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2022, 26(7): 777-783. doi: 10.16462/j.cnki.zhjbkz.2022.07.006

原发性干燥综合征患者唾液腺组织ceRNA调控网络的构建

doi: 10.16462/j.cnki.zhjbkz.2022.07.006
基金项目: 

国家自然科学基金 81573217

详细信息
    通讯作者:

    王斌,E-mail: wangbin@ahmu.edu.cn

  • 中图分类号: R593.2;R181

Construction and analysis of ceRNA network in salivary gland tissues of patients with primary Sjogren's syndrome

Funds: 

National Natural Science Foundation of China 81573217

More Information
  • 摘要:   目的  通过生物信息学方法构建原发性干燥综合征(primary Sjögren’s syndrome, pSS)患者唾液腺组织中的竞争内源性RNA(competing endogenous RNA, ceRNA)调控网络并探讨其发病机制。  方法  利用基因表达综合数据库(Gene Expression Omnibus, GEO)对pSS患者唾液腺组织中的信使RNA(messenger RNA, mRNA)、长链非编码RNA(long non-coding RNA, lncRNA)表达谱作差异分析。通过miRcode数据库和3个miRNA数据库(TargetScan、miRDB和miRWalk)获取与pSS有关的靶微小RNA(microRNA, miRNA)和靶mRNA,构建lncRNA-miRNA-mRNA的ceRNA调控网络,对ceRNA网络中差异表达基因(differentially expressed mRNAs, DEmRNAs)进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)通路富集分析。  结果  在ceRNA网络中有214个DEmRNAs。KEGG分析显示DEmRNAs的主要富集信号通路为丝裂原活化蛋白激酶(mitogen-activated protein kinase, MAPK)信号通路和腺苷酸活化蛋白激酶(AMP-activated protein kinase, AMPK)信号通路,DEmRNAs中10个枢纽(Hub)基因分别为JUNCCND1、NRASDDX5、KAT2BVEGFASIRT1、CLTAMCM7和RPS6KA1。  结论  本研究通过构建ceRNA调控网络,发现网络中DEmRNAs主要富集MAPK和AMPK信号通路, 为进一步深入探讨原发性干燥综合征的诊断和治疗提供了新的思路。
  • 图  1  mRNA和lncRNA差异表达分析结果

    注:A表示DEmRNAs火山图;B表示DElncRNAs火山图;C表示DEmRNAs热图;D表示DElncRNAs热图。

    Figure  1.  Differential expression analysis of mRNA and lncRNA

    图  2  pSS的ceRNA调控网络

    Figure  2.  The ceRNA regulation network of pSS

    图  3  KEGG通路富集分析

    注:A和C表示上调DEmRNAs;B和D表示下调DEmRNAs。

    Figure  3.  KEGG pathway enrichment analysis

    图  4  ceRNA调控网络中的枢纽基因

    Figure  4.  Hub genes in ceRNA regulation network

    图  5  PPI互作网络图

    Figure  5.  Protein-protein interaction networks

    表  1  GEO数据集基本信息

    Table  1.   The basic information of GEO datasets

    GSE编号 平台 病例(n) 对照(n) 样本类型 数据类型 疾病
    GSE23117 GPL570 11 4 唾液腺组织 mRNA pSS
    GSE76013 GPL21242 4 4 唾液腺组织 lncRNA pSS
    下载: 导出CSV

    表  2  pSS相关lncRNA的靶miRNA

    Table  2.   Target mirnas of pSS related lncrnas

    编号 lncRNA miRNA
    1 C1orf112 hsa-miR-503
    2 CEACAM21 hsa-miR-138
    3 TFAP2B hsa-miR-132
    4 CELSR3 hsa-miR-503
    5 TRAF3IP3 hsa-miR-132
    6 TNFRSF9 hsa-miR-132
    7 ANKRD44 hsa-miR-130ac
    8 RASGRP2 hsa-miR-503
    9 RPS6KA6 hsa-miR-130ac
    10 LINC00426 hsa-miR-130ac
    注:表中只列出符合条件的前10位lncRNA和miRNA。
    下载: 导出CSV

    表  3  pSS相关miRNA的靶mRNA

    Table  3.   Target mirnas of pSS related lncrnas

    编号 miRNA mRNA miRDB miRTarBase TargetScan
    1 hsa-miR-1297 DEPDC1 1 1 1
    2 hsa-miR-142-3p PSMB5 1 1 1
    3 hsa-miR-23b-3p GJA1 1 1 1
    4 hsa-miR-17-5p SMOC1 1 1 1
    5 hsa-miR-107 POLD3 1 1 1
    6 hsa-miR-761 ZNF641 1 1 1
    7 hsa-miR-20b-5p ZNF532 1 1 1
    8 hsa-miR-17-5p MORF4L1 1 1 1
    9 hsa-miR-20b-5p FAM46C 1 1 1
    10 hsa-miR-137 PTGS2 1 1 1
    注:表中只列出符合条件的前10位lncRNA和miRNA; 1表示基因在对应数据库中存在。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-10-31
  • 修回日期:  2022-02-15
  • 网络出版日期:  2022-07-19
  • 刊出日期:  2022-07-10

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