Analysis of urban community health elderly urine AD7c-NTP content
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摘要: 目的 通过对城市社区60岁以上老年人群尿液中神经丝蛋白(neuronal thread protein in the urine,AD7c-NTP)含量的测定,初步探索其在老年性痴呆(alzheimer's disease,AD)早期筛查诊断中的价值。方法 采用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA),检测病例组(86例)与年龄、性别相匹配的对照组(172例)尿液中AD7c-NTP的含量,绘制ROC曲线,计算灵敏度与特异度。结果 病例组尿AD7c-NTP值平均为(2.32±1.38)ng/ml,对照组尿AD7c-NTP值平均为(0.86±0.62)ng/ml,两组间差异具有统计学意义(t=9.383,P<0.001);ROC曲线下的面积为0.854,当临界值选定为1.313 ng/ml时,灵敏度为84.9%,特异度为70.3%。结论 尿液中AD7c-NTP的含量可作为AD早期筛查诊断的生化指标。Abstract: Objective To measure the content of neuronal thread protein in the urine (AD7c-NTP) of urban community elderly who are over 60 years, and to explore the value in the early screening and diagnosis of Alzheimer's disease (AD). Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the contents of urine AD7c-NTP between the case group (86 cases) and age-matched control group (172 cases), then ROC curve was drawn and sensitivity and specificity were calculated. Results The average levels of AD7c-NTP in case group were (2.32±1.38) ng/ml, and in the control group were (0.86±0.62) ng/ml. The difference between the two groups was statistically significant(t=9.383,P<0.001); The area under the ROC curve was 0.854. When the threshold was selected as 1.313 ng/ml, the sensitivity was 84.9%, and specificity was 70.3%. Conclusions The levels of AD7c-NTP in the urine can be used as biological indicators of early screening and diagnosis of AD.
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Key words:
- Aged /
- Alzheimer disease /
- ROC curve
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