Study on distribution of partial HIV-1 strains subtypes in Liuzhou City from 2011 to 2013 by gene gag sequencing
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摘要: 目的 了解2011-2013年柳州市人类免疫缺陷病毒1(human immunodeficiency virus 1, HIV-1)亚型分布、流行毒株情况。方法 从2011-2013年柳州市HIV-1毒株样本中采用单纯随机抽样方法抽取120株作为研究对象,将120份样本从血浆中抽提核酸RNA,扩增HIV-1 gag基因片段,送至北京六合华大基因科技股份有限公司测序。合格序列利用数据库分析亚型以及利用MEGA5构建系统进化树。结果 得到合格的gag基因片段98条序列。来自gag基因片段的序列分析重组流行毒株(circulate recombinant forms, CRFs)的重组亚型CRF01_AE占95.9%(94/98),异性传播比例为79.8%(75/94);重组亚型CRF07_BC占4.1%(4/98)。样本1LZ022和1LZ032,1LZ025和3LZ029的bootstrap值分别为99.0%,100.0%,两两之间传播途径不同。结论 2011-2013年,柳州市HIV-1以重组亚型CRF01_AE为主。柳州市重组亚型CRF01_AE从吸毒人群向异性接触感染人群蔓延。利用系统进化关系结合传播途径,便于分析传染源,为艾滋病干预措施提供有力佐证。
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关键词:
- HIV-1 /
- 基因,gag /
- 获得性免疫缺陷综合征
Abstract: Objective To understand human immunodeficiency virus 1(HIV-1) subtype distribution and pandemic strains in Liuzhou City from 2011 to 2013. Methods From 2011 to 2013, 120 samples which were randomly selected in HIV-1 infectors were extracted RNA nucleic acids from plasma, and were amplified gene gag fragment to be sequenced by BGI Technology Co. The qualified sequences were analyzed subtypes by using Los Alamos National Laboratory HIV database and built a phylogenetic tree by MEGA 5 software. Results The 98 sequences of gene gag were accomplished. The circulate recombinant forms's recombinant subtype CRF01_AE accounted for 95.9%(94/98), the heterosexual transmission accounted for 79.8% (75/94); and recombinant subtype CRF07_BC accounted for 4.1%(4/98). There were 1LZ022 and 1LZ032, 1LZ025 and 3LZ029 with high bootstrap value of 99.0% and 100.0% respectively between the two different transmission ways. Conclusions From 2011 to 2013, subtype of HIV-1 was mainly CRF01_AE which had spread from drug addicts to the heterosexual contact infections in Liuzhou City. Using phylogenetic tree combined with the transmission way, to locate the origin of infection and provide powerful evidence for HIV/AIDS intervention measures.-
Key words:
- HIV-1 /
- Genes,gag /
- Acquired immunodeficiency syndrome
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