Carriage rate and antibiotic resistance of multidrug-resistant S. aureus isolates among residents in Guangdong Province
-
摘要: 目的 调查广东省部分人群(动物从业人员和一般人群)的多重耐药金葡菌(multidrug-resistant S. aureus,MDRSA)携带情况,并分析菌株的耐药性。方法 对研究对象进行问卷调查和鼻拭子采样,对金葡菌进行药敏试验,分析方法采用χ2检验。结果 动物从业人员中金葡菌、MDRSA、甲氧西林耐药金葡菌(methicillin-resistant S. aureus,MRSA)的携带率均高于对照人群(分别为13.3% vs 9.3%;9.5% vs 3.4%;7.0% vs 1.4%;均有P<0.01)。金葡菌对青霉素、克林霉素、四环素和红霉素的耐药率较高,MDRSA主要耐药模式是同时对克林霉素、红霉素、四环素耐药。除了青霉素外,动物从业人员金葡菌对各类抗生素的耐药率均高于对照人群;MRSA菌株对各类抗生素的耐药率均高于甲氧西林敏感金葡菌。结论 本研究提示职业性动物接触可能导致从业人员感染耐药细菌。Abstract: Objective To investigate the carriage rate and antibiotic resistance of multidrug-resistant S. aureus among animal-related workers and general population in Guangdong Province. Methods We surveyed the study population by face-to-face questionnaire and nasal swab sampling. All S. aureus isolates were tested for antibiotic susceptibility test, and the data were analyzed by chi-squared test. Results The carriage rates of S. aureus, multidrug-resistant S. aureus and methicillin-resistant S. aureus (MRSA) were higher in animal-related workers than in controls (13.3% vs 9.3%; 9.5% vs 3.4%; 7.0% vs 1.4%; all P<0.01). The rates of S. aureus isolates showed high levels of resistance to penicillin, clindamycin, tetracycline and erythromycin, and the dominant multidrug resistance pattern of multidrug-resistant S. aureus isolates were mainly resistant to clindamycin, erythromycin and tetracycline. S. aureus strains isolated from animal-related workers showed higher levels of resistances to antibiotics, except for penicillin, than those from general population. The antibiotic-resistant rate was higher in MRSA isolates than in methicillin-sensitive S. aureus isolates. Conclusions This study suggests that occupational animal contact may lead to the infection of drug resistant bacteria.
-
Key words:
- Staphylococcus aureus /
- Occupational exposure /
- Epidemiologic methods
-
李莎莎,刘艳艳,李家斌. 2009-2011年阜阳地区常见病原菌分布和耐药性分析[J]. 中华疾病控制杂志, 2014,18(5):394-397. 刘伟东,叶小华,姚振江,等. 动物密切接触者耐甲氧西林金黄色葡萄球菌携带风险研究[J]. 中华疾病控制杂志, 2015,19(5):484-487. Datta R, Shah A, Huang SS, et al. High nasal burden of methicillin-resistant Staphylococcus aureus increases risk of invasive disease[J]. J Clin Microbiol, 2014,52(1):312-314. Klevens RM, Morrison MA, Nadle J, et al. Invasive methicillin-resistant Staphylococcus aureus infections in the United States[J]. JAMA, 2007,298(15): 1763-1771. Zhang K, Sparling J, Chow BL, et al. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci[J]. J Clin Microbiol, 2004,42(11):4947-4955. Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance[J]. Clin Microbiol Infect, 2012,18(3):268-281. Salgado CD, Farr BM, Calfee DP. Community-acquired methicillin-resistant Staphylococcus aureus: a meta-analysis of prevalence and risk factors[J]. Clin Infect Dis, 2003,36(2):131-139. Yan X, Song Y, Yu X, et al. Factors associated with Staphylococcus aureus nasal carriage among healthy people in Northern China[J]. Clin Microbiol Infect, 2015, 21(2):157-162. Ho PL, Chiu SS, Chan MY, et al. Molecular epidemiology and nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus among young children attending day care centers and kindergartens in Hong Kong[J]. J Infect, 2012,64(5):500-506. Lee J, Sung JY, Kim YM, et al. Molecular characterization of methicillin-resistant Staphylococcus aureus obtained from the anterior nares of healthy Korean children attending daycare centers[J]. Int J Infect Dis, 2011, 15(8):e558-e563. Fang HW, Chiang PH, Huang YC. Livestock-associated methicillin-resistant Staphylococcus aureus ST9 in pigs and related personnel in Taiwan[J]. PLoS One, 2014,9(2):e88826. Boost M, Ho J, Guardabassi L, et al. Colonization of butchers with livestock-associated methicillin-resistant Staphylococcus aureus[J]. Zoonoses Public Health, 2013,60(8):572-576. Gilbert MJ, Bos ME, Duim B, et al. Livestock-associated MRSA ST398 carriage in pig slaughterhouse workers related to quantitative environmental exposure[J]. Occup Environ Med, 2012,69(7):472-478. Smith TC, Male MJ, Harper AL, et al. Methicillin-resistant Staphylococcus aureus (MRSA) strain ST398 is present in midwestern U.S. swine and swine workers[J]. PLoS One, 2009,4(1):e4258. Leibler JH, Jordan JA, Brownstein K, et al. Staphylococcus aureus nasal carriage among beefpacking workers in a Midwestern United States Slaughterhouse[J]. PLoS One, 2016,11(2):e0148789. Brennan GI, Abbott Y, Burns A, et al. The emergence and spread of multiple livestock associated clonal complex 398 methicillin-resistant and methicillin-susceptible staphylococcus aureus strains among animals and humans in the Republic of Ireland, 2010-2014[J]. PLoS One, 2016,11(2):e0149396. Narvaez-Bravo C, Toufeer M, Weese SJ, et al. Prevalence of methicillin-resistant Staphylococcus aureus in Canadian commercial pork processing plants[J]. J Appl Microbiol, 2016,120(3):770-780. Bisdorff B, Scholholter JL, Claussen K, et al. MRSA-ST398 in livestock farmers and neighbouring residents in a rural area in Germany[J]. Epidemiol Infect, 2012,140(10):1800-1808. Vandendriessche S, Vanderhaeghen W, Soares FV, et al. Prevalence, risk factors and genetic diversity of methicillin-resistant Staphylococcus aureus carried by humans and animals across livestock production sectors[J]. J Antimicrob Chemother, 2013,68(7):1510-1516. Mulders MN, Haenen AP, Geenen PL, et al. Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The Netherlands[J]. Epidemiol Infect, 2010,138(5):743-755. Liu W, Liu Z, Yao Z, et al. The prevalence and influencing factors of methicillin-resistant Staphylococcus aureus carriage in people in contact with livestock: A systematic review[J]. AM J Infect Control, 2015,43(5):469-475.
点击查看大图
计量
- 文章访问数: 328
- HTML全文浏览量: 63
- PDF下载量: 0
- 被引次数: 0