Abstract: Objective This study aims to estimate the disease burden of norovirus gastroenteritis in Guangzhou in 2014, and provide scientific evidence for the government's decision-making and control measures for infectious gastroenteritis and foodborne diseases. Methods The study was conducted by separately estimating the disease burden of both the outbreak and sporadic situations of norovirus gastroenteritis in Guangzhou. The indicators include disability-adjusted life years, morbidity, hospitalisation rate and morbidity. Data was acquired from monitoring hospitals, other research literature and the "public health emergency management information system". Results The total number of diarrhoea cases was approximately 3 092 230 in Guangzhou in 2014, of which 608 860 cases were related to a norovirus infection. The annual incidence of norovirus gastroenteritis was 4 655/100 000 people, with a hospitalisation rate of 0.03%. There were also six cases of outbreaks in Guangzhou in 2014, with an average prevalence of 2.63%. Integrating the data from sporadic and outbreak situations, the estimated disease burden of norovirus gastroenteritis in Guangzhou in 2014 was 2 212 DALYs. Conclusions The disease burden of norovirus gastroenteritis in Guangzhou is high, indicating that relevant health departments should strengthen their prevention, control and management efforts for norovirus gastroenteritis infections, in addition to increasing education and awareness.