Abstract: Objective To assess the knowledge, attitude, and practice of children's guardians in ethnic minority areas of Qinghai Province on children vaccination and related influencing factors, and to understand efficient channels for vaccination information dissemination and communication. Methods Using the method of stratified random sampling, 240 children aged 0-6 years were selected from Xunhua County, Qinghai Province. Questionnaires were filled by the children's guardians. The data were analyzed by chi-square test and logistic regression models. Results Getting access of vaccination information (χ²=6.64, P=0.040) and KAP score (χ²=5.56, P=0.020) were the influencing factors for the children not or late vaccinated. Rural doctors were a main channel for the guardians obtaining vaccination related knowledge (93.75%), followed by relatives, friends, and colleagues (72.50%). The guardians trusted more on the vaccination knowledge from rural doctors, religious figures, and village cadres. Conclusions The guardians have insufficient vaccination related knowledge, attitude, and practice. In ethnic minority areas, enhanced dissemination and communication are needed to improve the awareness of vaccination of children's guardians. Village doctors, religious figures, village cadres, and relatives/friends might be appropriate to communicate vaccination information.