Abstract: Objective To investigate infection status and distribution of different types of human papillomavirus (HPV) in women of different ages in Hanzhong area of Shanxi Province. Methods Twenty-three HPV genotypes were analyzed by PCR and reverse dot blot hybridization technique. Results The total infection rate, high-risk HPV infection rate, low-risk HPV infection rate and mixed high and low risk infection rate were 29.86%, 26.79%, 1.57% and 1.49% respectively. The infection rate of single high-risk subtype of HPV and multiple subtype of HPV were 21.08% and 5.71% respectively. HPV infection rate was higher for women under 20 years old and over 60 years old groups and the infection rate gradually increased along with women's age in above 21 years old age groups. High-risk HPV subtype infection rate was the highest in 50 -years old group. The most common subtype were 16,58,52,53,33 and 39 in total and single subtype infection and its infection rates were 73.25% and 79.71% in HPV infection respectively and subtype 16 reached 27.11% and 34.41% in all HPV infection respectively. Single and high-risk single type infection rates were 75.66% and 70.59% in total HPV infection respectively. Multiple and high-risk multiple infection rates were 24.34% and 24.13% in total infection respectively. Conclusions The HPV infection rate of women in Hanzhong region of Shanxi province is higher especially in the over 60 years old age group and the most common HPV high and low risk subtypes are 16,58,52,53,33,39 and 6,43. The infection rates of total, high-risk, low-risk and high-low-risk were significantly different in different age groups.