Abstract: Objective To investigate symptom of patients with lung cancer during chemotherapy and their incidence and severity level, for providing a proper management procedure for the patients. Methods A total of 150 lung cancer patients with chemotherapy were investigated by the Chinese version of the memorial symptom assessment scale (MSAS-Ch). Principal component analysis was performed to conduct the exploratory factor analysis. Results The average age of all the patients was (61.8±9.7) years old, and 66.7% were male. The cultural degree was mainly junior high school and under (85.4%). The highest incidence symptom of patients with chemotherapy was ‘worrying’, and the ‘pain’ was most severe. Five common factors were extracted from the MSAS-Ch, explaining 60.47% variance. The factors were intestinal discomfort symptom cluster, fatigue-related symptom cluster, psychology illness symptom cluster, sickness behavior symptom cluster, adverse reaction symptom cluster. Conclusions Clinical nursing staff should focus on general characteristics of patients and find out the source and key point of symptoms, utilize the synergistic effect of symptoms clusters and do well in recognition work, put forward targeted management procedures to improve life quality of lung cancer patients.