Abstract: Objective To analyze the cause of death and life loss caused by injury in Jinshan District of Shanghai from 1990 to 2016 and provide suggestion for making strategy of prevention and control of injury. Methods The data was extracted from injury death registry database of Jinshan Distract; crude death rate, standardized death rate, potential years of life loss (PYLL), rate of potential years of life loss (PYLLR), standardized rate of potential years of life loss (SPYLLR) during 27 years were analyzed. Results In Jinshan district from 1900 to 2016, the injury-specific death rate was 51.39 per 100 thousands and the proportion of death of injury was 7.36% and ranked the 4th death cause. PYLLR of injury was 13.24‰ and was the 2nd death cause ranked by PYLLR. In the past 27 years, injury standardized death rate, PYLL and SPYLLR presented declining trend. The first four rank of injury death cause were traffic accident, falls, suicide and accidental drowning. The first rank of death cause of injury in 0-years old group was accidental drowning, its age-specific death rate was 11.24 per 100 thousands; In 15-years old group was traffic accident, its age-specific death rate was 21.21 per 100 thousands; In 65 years old group was falls, its age-specific death rate was 59.78 per 100 thousands; Ranked by PYLLR the first four death cause of injury were traffic accident, accidental drowning, suicide and falls, the PYLLR of the first four death cause ranged from 1.12‰ to 6.36‰. Conclusions Injury has become a serious public health problem which threatened badly to the residents' life security in Jinshan District. We should take targeted measures to prevent and control the occurrence of injuries.