Investigation on the carriage of Zika Virus, Dengue Virus and Rabies Virus in bats in Guangdong Province
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摘要: 目的 了解广东地区蝙蝠携带狂犬病病毒、寨卡病毒以及登革热病毒情况。方法 2014-2016年分别在广州市和湛江市部分地区采集蝙蝠，取其血清和脑组织标本，采用实时荧光定量聚合酶链式反应法（real-time quantitative polymerase chain reaction，qRT-PCR）、巢式聚合酶链反应（nested polymerase chain reaction，Nested PCR），酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）和细胞培养分离检测所采标本中的狂犬病病毒、登革热病毒和寨卡病毒的感染情况。结果 采集蝙蝠共174只，分属2科3个种：果蝠科的犬蝠属（Cynopterus sphinx）33只、蝙蝠科的普通伏翼属（Pipistrellusabramus）9只及高头蝠属（Scotophiluskuhlii）132只。分别取得血清、脑组织各174份。在犬蝠脑标本中检出登革热病毒阳性1份，阳性率0.06%（1/174），寨卡病毒、狂犬病病毒均未检出。结论 广东两个地区的3种蝙蝠作为寨卡病毒、登革热病毒和狂犬病病毒宿主的可能性较小。Abstract: Objective To investigate the prevalence of Zika virus, Dengue virus and Rabies virus in bats in Guangdong Province.Methods The Real-time quantitative polymerase chain reaction(qRT-PCR), Nested polymerase chain reaction(Nested PCR), enzyme linked immunosorbent assay(ELISA) and cell culture were used to detect the three viruses in serum and brain tissue samples obtained from the bats from January 2014 to December 2016.Results There were 174 serums and brain tissues of the bats from two families and three species, including 33 Cynopterus sphinx belonging to Pteropodidae, 132 Scotophiluskuhlii and 9 Pipistrellusabramus belonging to Vespertilionidae. One brain sample was tested positive for dengue virus in Cynopterussphinx, the positive rate was 0.06% (1/174), while zika virus and rabies virus were not detected.Conclusion The three species of bats from Guangzhou are less likely to be the reservoir of zika virus, dengue virus and rabies virus.
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Key words:
- Bats /
- Rabies virus /
- Dengue virus /
- Zika virus
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