长链非编码RNA SFTA1P在非小细胞肺癌组织中的表达和细胞功能研究

周玫余; 袁帅; 向颖; 吴龙; 邬娜; 李成英; 许斌; 张耀; 蔡同建; 马翔宇; 余祖滨; 白莉; 杨敬源; 李亚斐

doi:10.16462/j.cnki.zhjbkz.2020.03.018

长链非编码RNA SFTA1P在非小细胞肺癌组织中的表达和细胞功能研究

doi: 10.16462/j.cnki.zhjbkz.2020.03.018

周玫余^{1, 2},
袁帅²,
向颖²,
吴龙²,
邬娜²,
李成英²,
许斌²,
张耀²,
蔡同建²,
马翔宇²,
余祖滨³,
白莉⁴,
杨敬源¹,
李亚斐^2, ,

1.
550000 贵阳, 贵州医科大学公共卫生学院流行病学教研室
2.
400038 重庆, 陆军军医大学军事预防医学系军队流行病学教研室
3.
400037 重庆, 第三军医大学新桥医院胸外科
4.
400037 重庆, 第三军医大学新桥医院呼吸内科

基金项目: 国家自然科学基金面上项目(81871896, 81672316);重庆市自然科学基金(CSTC2015jcyBX0110)

详细信息

通讯作者:
李亚斐, E-mail:liyafei2008@hotmail.com

中图分类号: R734.2
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- 被引次数: 0
出版历程
- 收稿日期: 2019-09-05
- 修回日期: 2019-12-01
- 刊出日期: 2020-03-10

Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer

1.
Department of Epidemiology, School of Public Health, Guizhou Medical University, Guiyang 550000, China
2.
Department of Epidemiology, College of Preventive Medicine, Army Medical University, Chongqing 400038, China
3.
Department of Thoracic Surgery, Xinqiao Hospital, Army Medical university, Chongqing 400037, China
4.
Department of Respiratory Disease, Xinqiao Hospital, Army Medical university, Chongqing 400037, China

Funds: The General Program of National Natural Science Foundation of China(81871896, 81672316); The Natural Science Foundation of Chongqing(CSTC2015jcyBX0110)

More Information

Corresponding author: LI Ya-fei, E-mail:liyafei2008@hotmail.com

摘要

摘要: 目的研究长链非编码RNA SFTA1P在非小细胞肺癌(non-small cell lung cancer, NSCLC)中的表达情况, 以及对NSCLC细胞系生物学功能的影响。方法运用定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction, qRT-PCR)检测SFTA1P在18对非小细胞肺癌组织及癌旁组织中的表达情况; 用qRT-PCR检测5株NSCLC细胞系(A549、SPCA1、H1975、H460和H1299)和1株肺正常上皮细胞系(human bronchial epithelial, HBE)中SFTA1P的表达情况; 构建SFTA1P的过表达载体, 通过转染构建过表达细胞模型; 运用CCK-8、Transwell等实验方法检测SFTA1P过表达对NSCLC细胞增殖、侵袭、迁移的影响。结果 SFTA1P在非小细胞肺癌组织中的表达低于癌旁组织(t=2.158, P=0.043);与HBE细胞相比较, SFTA1P在A549和H460细胞系中相对低表达(t=5.769, P=0.004; t=5.772, P=0.004), 在H1299和H1975细胞系中相对高表达(t=22.248, P < 0.001; t=11.814, P < 0.001);SFTA1P过表达质粒转染A549和H460细胞系后, 与空质粒的对照组比较, SFTA1P的表达均升高, 且有统计学差异(均有P < 0.05);过表达SFTA1P可以抑制非小细胞肺癌细胞H460、A549的增殖、侵袭、迁移, 差异有统计学意义(均有P < 0.05)。结论 SFTA1P基因能够抑制NSCLC细胞的增殖、迁移、侵袭等生物学功能, SFTA1P在肿瘤发生和发展中可能起着抑癌基因的作用。
- NSCLC /
- 长链非编码RNA /
- SFTA1P /
- 细胞增殖 /
- 细胞迁移和侵袭
Abstract: Objective To investigate the expression of long non-coding RNA SFTA1 P in non-small cell lung cancer(NSCLC)and its biological function in NSCLC cell lines. Methods Quantitative real time polymerase chain reaction(qRT-PCR) was used to detect the expression of SFTA1 P in 18 pairs of NSCLC tissues and adjacent normal tissues. The expression of SFTA1 P was detected by qRT-PCR in five different NSCLC cell lines(A549, SPCA1, H460, H1975 and H1299) and one normal lung epithelial cell line(HBE). The overexpression vector of SFTA1 P was designed and constructed. The overexpressed cell line was constructed by transfection, the effects of overexpression of SFTA1 P on proliferation, invasion and migration of NSCLC cells were detected by CCK-8 assay and transwell assay. Results The expression of SFTA1 P in NSCLC tissues was lower than that of adjacent normal tissues(t=2.158, P=0.043). SFTA1 P expression was detected in 5 strains of NSCLC cell lines and normal lung epithelial cell line. The expression of SFTA1 P was the lowest in A549 and H460 cell lines(t=5.769, P=0.004;t=5.772, P=0.004), and the highest in H1299 and H1975 cell lines(t=22.248, P < 0.001;t=11.814, P < 0.001). SFTA1 P overexpression cell models were successfully constructed using A549 and H460 cell lines(allP < 0.05). The overexpression of SFTA1 P could inhibit proliferation, invasion and migration of H460 and A549 cells((allP < 0.05). Conclusions SFTA1 P can affect the biological functions of NSCLC cells by inhibiting the proliferation, migration and invasion. SFTA1 P may play a role as a tumor suppressor gene in tumorigenesis and development.
- Non-small cell lung cancer /
- Long non-coding RNA /
- SFTA1P /
- Cell proliferation /
- Cell migration

HTML全文

图 1 SFTA1P在癌组织与正常肺组织中的表达差异和SFTA1P在NSCLC细胞系中的表达情况

注:A:SFTA1P在癌组织与正常组织中的表达差异, a表示与正常的癌旁组织比较, P < 0.05;B:SFTA1P在各细胞系中的表达情况, a表示与HBE比较, P < 0.05, b表示与HBE比较, P < 0.01。

Figure 1. The expression of SFTA1P in lung cancer tissues and normal tissues and the expression of SFTA1P in NSCLC cell lines

下载: 全尺寸图片幻灯片

图 2 SFTA1P过表达效果的鉴定及CCK-8检测SFTA1P过表达对细胞增殖能力的影响

注:A: pcDNA3.1-SFTA1P转至A549细胞, qPCR检测SFTA1P表达量; B: pcDNA3.1-SFTA1P转至H460细胞, qPCR检测SFTA1P表达量; C: A549过表达后增殖情况; D: H460过表达后增殖情况; a表示与pcDNA3.1比较, P < 0.05, b表示与pcDNA3.1比较, P < 0.01。

Figure 2. Identification of SFTA1P overexpression effect and CCK-8 detection of SFTA1P overexpression effect on cell proliferation

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图 3 SFTA1P过表达对NSCLC细胞系迁移和侵袭的影响

注:A: A549; B:H460, 两组细胞分别转染pcDNA3.1-SFTA1P后, 迁移、侵袭实验结果(结晶紫×200);a表示与pcDNA3.1比较, P < 0.05, b表示与pcDNA3.1比较, P < 0.01。

Figure 3. Effects of SFTA1P overexpression on cell migration and invasion of NSCLC cell lines

下载: 全尺寸图片幻灯片

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