-
摘要:
目的 探讨幽门螺杆菌(Helicobacter pylori, H.pylori)感染对血清胃泌素及外泌体源性胃泌素的影响。 方法 随机收集79例慢性胃炎患者血清，13C呼气试验判断H.pylori感染情况，ELISA法测定血清胃泌素和外泌体源性胃泌素浓度。 结果 血清胃泌素及外泌体源性胃泌素在非萎缩性胃炎(non-atrophic gastritis, NAG)与萎缩性胃炎(atrophic gastritis, AG)两组患者中差异均无统计学意义(t=-0.197, P=0.844; t=0.221, P=0.826)。在NAG患者中，H.pylori阳性亚组血清胃泌素及外泌体源性胃泌素均低于H.pylori阴性亚组(t=2.488, P=0.017; t=2.655, P=0.012)。而在AG患者中，血清胃泌素浓度在H.pylori阳性与阴性两亚组中差异无统计学意义(t=1.888, P=0.082)；但外泌体源性胃泌素在H.pylori阳性亚组高于H.pylori阴性亚组(t=2.394, P=0.029)。 结论 H.pylori感染早期、胃黏膜发生萎缩前，血清胃泌素下降；当H.pylori持续感染导致萎缩后，血清外泌体源性胃泌素分泌增加，胃泌素多以外泌体形式存在，并可能参与了H.pylori感染致病分子机制。 Abstract:Objective To investigate the effects of Helicobacter pylori (H.pylori) infection on serum gastrin and exosome derived gastrin secretion. Methods A total of 79 patients with non-atrophic gastritis (NAG) and atrophic gastritis (AG) were collected randomly. The infection of H.pylori was detected by a 13C-urea breath test. The concentration of serum gastrin and serum exosome derived gastrin were detected by enzyme-linked immunosorbent assay(ELISA). Results There was no significant difference in serum gastrin and exosome derived gastrin between NAG and AG group(t=-0.197, P=0.844; t=0.221, P=0.826). In NAG group, serum gastrin and exosome derived gastrin in H.pylori positive subgroup were significantly lower than those in H.pylori negative subgroups (t=2.488, P=0.017; t=2.655, P=0.012). In AG group, there was no significant difference in serum gastrin concentration between H. pylori positive and negative subgroups (t=1.888, P=0.082). However, exosome derived gastrin in H.pylori positive subgroup was significantly higher than that in H.pylori negative subgroup (t=2.394, P=0.029). Conclusions H.pylori infection may lead to the decrease of serum gastrin before gastric mucosal atrophy in the early stage. Serum gastrin and exosome derived gastrin in H. pylori positive AG group are significantly increased, which is an important carcinogenic mechanisms of H. pylori infection. -
Key words:
- Helicobacter pylori /
- Gastrin /
- Exosomes /
- Atrophic gastritis /
- Gastric carcinogenesis
-
表 1 NAG与AG组血清胃泌素浓度比较(x±s)
Table 1. Comparison of serum gastrin concentrations between NAG and AG groups (x±s)
组别 例数(例) 血清胃泌素(pg/mL) t值 P值 NAG 49 37.38±21.06 -0.197 0.844 AG 26 38.38±20.45 
下载: 导出CSV
表 2 NAG与AG组外泌体源性胃泌素浓度比较(x±s)
Table 2. Comparison of exosome derived gastrin concentrations between NAG and AG group (x±s)
组别 例数(例) 血清胃泌素(pg/mL) t值 P值 NAG 51 84.38±59.81 0.221 0.826 AG 27 81.66±35.84
下载: 导出CSV
表 3 H.pylori感染对慢性胃炎患者血清胃泌素的影响(x±s)
Table 3. Effect of H.pylori infection on serum gastrin in patients with chronic gastritis (x±s)
组别 例数(例) 血清胃泌素(pg/mL) t值 P值 NAG 2.488 0.017 H.pylori阴性 25 44.09±24.09 H.pylori阳性 24 30.37±13.94 AG 1.888 0.082 H.pylori阴性 13 30.76±8.38 H.pylori阳性 13 46.19±27.14
下载: 导出CSV
表 4 H.pylori感染对慢性胃炎患者血清外泌体源性胃泌素的影响(x±s)
Table 4. Effect of H.pylori infection on serum exosome derived gastrin in patients with chronic gastritis (x±s)
组别 例数(例) 血清胃泌素(pg/mL) t值 P值 NAG 2.655 0.012 H.pylori阴性 26 102.59±31.24 H.pylori阳性 25 63.16±30.71 AG 2.394 0.029 H.pylori阴性 14 65.76±18.38 H.pylori阳性 13 97.23±43.69
下载: 导出CSV
-
[1] Waldum H, Fossmark R. Gastritis, gastric polyps and gastric cancer[J]. Int J Mol Sci, 2021, 22(12): 6548. DOI: 10.3390/ijms22126548. [2] Waldum HL, Rehfeld JF. Gastric cancer and gastrin: on the interaction of Helicobacter pylori gastritis and acid inhibitory induced hypergastrinemia[J]. Scand J Gastroenterol, 2019, 54(9): 1118-1123. DOI: 10.1080/00365521.2019.1663446. [3] Shao Y, Sun K, Xu W, et al. Helicobacter pylori infection, gastrin and cyclooxygenase-2 in gastric carcinogenesis[J]. World J Gastroenterol, 2014, 20(36): 12860-12873. DOI: 10.3748/wjg.v20.i36.12860. [4] Khajeh S, Tohidkia MR, Aghanejad A, et al. Phage display selection of fully human antibody fragments to inhibit growth-promoting effects of glycine-extended gastrin 17 on human colorectal cancer cells[J]. Artif Cells Nanomed Biotechnol, 2018, 46(sup2): 1082-1090. DOI: 10.1080/21691401.2018.1478846. [5] Osborne N, Sundseth R, Burks J, et al. Gastrin vaccine improves response to immune checkpoint antibody in murine pancreatic cancer by altering the tumor microenvironment[J]. Cancer Immunol Immunother, 2019, 68(10): 1635-1648. DOI: 10.1007/s00262-019-02398-6. [6] Meng LL, Wang JL, Xu SP, et al. Low serum gastrin associated with ER(+) breast cancer development via inactivation of CCKBR/ERK/P65 signaling[J]. BMC Cancer, 2018, 18(1): 824. DOI: 10.1186/s12885-018-4717-7. [7] Li Y, Zhao Y, Li Y, et al. Gastrin-17 induces gastric cancer cell epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway[J]. J Physiol Biochem, 2021, 77(1): 93-104. DOI: 10.1007/s13105-020-00780-y. [8] Waldum HL, Rehfeld JF. Gastric cancer and gastrin: on the interaction of Helicobacter pylori gastritis and acid inhibitory induced hypergastrinemia[J]. Scand J Gastroenterol, 2019, 54(9): 1118-1123. DOI: 10.1080/00365521.2019.1663446. [9] Liu W, Sun Y, Yuan Y. Analysis of serum gastrin-17 and Helicobacter pylori antibody in healthy Chinese population[J]. J Clin Lab Anal, 2020, 34(12): e23518. DOI: 10.1002/jcla.23518. [10] Takaishi S, Cui G, Frederick DM, et al. Synergistic inhibitory effects of gastrin and histamine receptor antagonists on Helicobacter-induced gastric cancer[J]. Gastroenterology, 2005, 128(7): 1965-1983. DOI: 10.1053/j.gastro.2005.03.027. [11] Waldum H. Gastrin drives gastric cancer due to oxyntic atrophy also after Helicobacter pylori eradication[J]. Therap Adv Gastroenterol, 2020, 13: 1756284820931717. DOI: 10.1177/1756284820931717. [12] Zagari RM, Rabitti S, Greenwood DC, et al. Systematic review with meta-analysis: diagnostic performance of the combination of pepsinogen, gastrin-17 and anti-Helicobacter pylori antibodies serum assays for the diagnosis of atrophic gastritis[J]. Aliment Pharmacol Ther, 2017, 46(7): 657-667. DOI: 10.1111/apt.14248. [13] Graham DY. Letter: questions regarding the diagnostic performance of serum assays for atrophic gastritis[J]. Aliment Pharmacol Ther, 2017, 46(11-12): 1117-1118. DOI: 10.1111/apt.14339. [14] Wang R, Chen XZ. Prevalence of atrophic gastritis in southwest China and predictive strength of serum gastrin-17: A cross-sectional study (SIGES)[J]. Sci Rep, 2020, 10(1): 4523. DOI: 10.1038/s41598-020-61472-7. [15] Han ML, Liou JM, Ser KH, et al. Changes of serum pepsinogen level and ABC classification after bariatric surgery[J]. J Formos Med Assoc, 2021, 120(6): 1377-1385. DOI: 10.1016/j.jfma.2020.10.029. [16] Wang Y, Liu X, Wang L, et al. A comparative study on changes in intestinal flora, pepsinogen and gastrin in patients with gastric cancer and atrophic gastritis[J]. J BUON, 2020, 5(2): 995-1000. [17] Chen XZ, Zhang WH, Chen HN, et al. Associations between serum CA724 and HER2 overexpression among stage Ⅱ-Ⅲ resectable gastric cancer patients: an observational study[J]. Oncotarget, 2016, 7: 23647-23657. DOI: 10.18632/oncotarget.8145. [18] Fu M, Gu J, Jiang P, et al. Exosomes in gastric cancer: roles, mechanisms, and applications[J]. Mol Cancer, 2019, 18(1): 41. DOI: 10.1186/s12943-019-1001-7. [19] Xia X, Zhang L, Chi J, et al. Helicobacter pylori infection impairs endothelial function through an exosome-mediated mechanism[J]. J Am Heart Assoc, 2020, 9(6): e014120. DOI: 10.1161/JAHA.119.014120. [20] Wang X, Ling L, Li S, et al. The diagnostic value of gastrin-17 detection in atrophic gastritis: a meta-analysis[J]. Medicine, 2016, 95(18): 1-9. DOI: 10.1097/MD.0000000000003599. [21] Liu W, Sun Y, Yuan Y. Analysis of serum gastrin-17 and Helicobacter pylori antibody in healthy Chinese population[J]. J Clin Lab Anal. 2020, 34(12): e23518. DOI: 10.1002/jcla.23518. [22] Parhusip DH, Siregar GA, Dairi LB. The difference of serum gastrin-17 level based on gastritis severity and Helicobacter pylori infection[J]. Maced J Med Sci, 2019, 7(8): 1266-1269. DOI: 10.3889/oamjms.2019.325. -
- 中国精品科技期刊
- 《中文核心期刊要目总览》收录期刊
- RCCSE 中国核心期刊(5/114,A+)
- Scopus收录期刊
- 美国《化学文摘》(CA)收录期刊
- WHO 西太平洋地区医学索引(WPRIM)收录期刊
- 《中国科学引文数据库(CSCD)》核心库期刊 (C)
- 中国科技核心期刊
- 中国科技论文统计源期刊
- 《日本科学技术振兴机构数据库(中国)》(JSTChina)收录期刊
- 美国《乌利希期刊指南》(UIrichsweb)收录期刊
- 中华预防医学会系列杂志优秀期刊(2019年)
留言板
尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!
| 姓名 | |
|---|---|
| 邮箱 | |
| 手机号码 | |
| 标题 | |
| |
