Expression and significance of aryl hydrocarbon receptor in peripheral blood CD4<sup>+</sup> CD25<sup>+</sup>T of rheumatoid arthritis

CHENG Lin; QIAN Long; TAN Yue; LI Xiang-pei; LI Xiao-mei; WANG Guo-sheng

doi:10.16462/j.cnki.zhjbkz.2016.04.014

Volume 20 Issue 4

Apr. 2016

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2016 > 20(4): 379-382

CHENG Lin, QIAN Long, TAN Yue, LI Xiang-pei, LI Xiao-mei, WANG Guo-sheng. Expression and significance of aryl hydrocarbon receptor in peripheral blood CD4+ CD25+T of rheumatoid arthritis[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(4): 379-382. doi: 10.16462/j.cnki.zhjbkz.2016.04.014

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CHENG Lin, QIAN Long, TAN Yue, LI Xiang-pei, LI Xiao-mei, WANG Guo-sheng. Expression and significance of aryl hydrocarbon receptor in peripheral blood CD4⁺ CD25⁺T of rheumatoid arthritis[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(4): 379-382. doi: 10.16462/j.cnki.zhjbkz.2016.04.014

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Expression and significance of aryl hydrocarbon receptor in peripheral blood CD4⁺ CD25⁺T of rheumatoid arthritis

doi: 10.16462/j.cnki.zhjbkz.2016.04.014

Department of Rheumatology and Immunology, Affiliated Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, China

Received Date: 2015-11-01
Rev Recd Date: 2016-01-20

Abstract

Abstract

Objective To explore the significance of aryl hydrocarbon receptor (AHR) in the pathogenesis of rheumatoid arthritis(RA) by detecting the percentage of AHR positive in CD4⁺ CD25⁺T cells. Methods Peripheral blood of 35 patients with RA and 14 healthy controls were collected. Flow cytometry (FCM) technology was applied to detect the proportion of CD4⁺ CD25⁺T cells and AHR positive cells in peripheral blood of each individual and the percentage of AHR positive cells in CCR6⁺CD4⁺T cells were also tested. Clinical data was recorded in detail and disease activity score in 28 joints (DAS28 ) was calculated. Then, the association between the percentage of AHR positive cells in CD4⁺ CD25⁺T cells and clinical data was analyzed. Results In peripheral blood of RA patients, the percentage of AHR positive cells in CD4⁺CD25⁺T cells was significantly lower than that in healthy controls ((17.90(6.10,80.10))% vs.(52.43(19.18,96.43))%,Z=-4.362,P<0.001); In peripheral blood of healthy controls, the percentage of AHR positive cells in CD4⁺CD25⁺T cells was significantly higher than that in CCR6⁺CD4⁺Tcells, and was also significantly higher than that in peripheral blood mononuclear cells(PBMCs) ((52.49±19.18)% vs. (23.18±5.62)% vs. (18.06±7.80)%, χ²=24.03, P<0.001); In peripheral blood of RA patients, the percentage of AHR positive cells in CD4⁺CD25⁺T cells was significantly lower than that in CCR6⁺CD4⁺T cells, and was also significantly lower than that in PBMCs((17.90(6.10,80.10))% vs. ((46.15(20.18,87.31))%vs. (34.43(8.72,56.37))%, χ²=38.29, P<0.001); Nevertheless, no statistically significant correlation was found between clinical data and the percentage of AHR positive cells in CD4⁺ CD25⁺T cells. Conclusions AHR may participate in the pathogenesis of RA by controlling the differentiation of Th17 and Treg cells .
- Arthritis,rheumatoid,
- Receptors,aryl hydrocarbon,
- T-lymphocytes

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References(19)

References

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