Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City

FENG Mei; LIU You-zhao; HE Ya-qing; LIU Zhi-hua; CHEN Qing; HU Jing

doi:10.16462/j.cnki.zhjbkz.2016.08.003

Volume 20 Issue 8

Aug. 2016

Turn off MathJax

Article Contents

Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2016 > 20(8): 764-767

FENG Mei, LIU You-zhao, HE Ya-qing, LIU Zhi-hua, CHEN Qing, HU Jing. Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(8): 764-767. doi: 10.16462/j.cnki.zhjbkz.2016.08.003

Citation:

FENG Mei, LIU You-zhao, HE Ya-qing, LIU Zhi-hua, CHEN Qing, HU Jing. Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(8): 764-767. doi: 10.16462/j.cnki.zhjbkz.2016.08.003

Citation:

FENG Mei, LIU You-zhao, HE Ya-qing, LIU Zhi-hua, CHEN Qing, HU Jing. Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(8): 764-767. doi: 10.16462/j.cnki.zhjbkz.2016.08.003

PDF( 920 KB)

Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City

doi: 10.16462/j.cnki.zhjbkz.2016.08.003

1. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangdong 510515, China;
2. Microbiology Inspection Division, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China;
3. Department of Infectious Diseases, Nanfang Hospital, Guangdong 510515, China

Received Date: 2016-04-07
Rev Recd Date: 2016-06-28

Abstract

Abstract

Objective To investigate the contamination status of Laribacter hongkongensis in freshwater products in Nanshan district of Shenzhen city, and to analyze the antibiotic resistant characteristic. Methods 347 freshwater product samples from five different aquatic product markets were collected during April to September 2015 in Shenzhen city. All the samples were inoculated by using cefoperazone macconkey agar (CMA). Suspicious isolates were identified by gram staining, biochemical tests, amplification and sequencing of 16S rRNA. Antimicrobial resistance of L. hongkongensis to 19 antimicrobial agents was tested using disc-diffusion (K-B) method to determine the antibiotic susceptibility patterns. Results A total of 108 (31.12%)strains were isolated among 347 samples of aquatic product. The positive rates of grass carps and tiger frogs were 20.44% and 50.82%, respectively. Comparison of drug susceptibilities between fish-derived strains and frog-derived strains showed that the higher proportion of fish strains than frog strains were the first-generation and second-generation cephalosporins, while the higher discrepancies for frog strains were observed with tetracycline, quinolones and sulfonamides. Among 38 multiple drug-resistant strains of L. hongkongensis, nine fish strains and 29 frog strains were presented 6 and 20 different resistant phenotype, respectively. Conclusions There are different levels of the contamination of L. hongkongensis in aquatic products in Shenzhen. In addition, strains derived from frog have more complex drug-resistant pattern.
- Fresh water,
- Drug resistance, microbial,
- Polymerase chain reaction

FullText(HTML)

References(16)

References

Marcos LA, DuPont HL. Advances in defining etiology and new therapeutic approaches in acute diarrhea [J]. J Infect, 2007,55(5):385-393.

Yuen KY, Woo PC, Teng JL, et al. Laribacter hongkongensis gen. nov., sp. nov., a novel gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema [J]. J Clin Microbiol, 2001,39(12):4227-4232.

Woo PC, Lau SK, Teng JL, et al. Association of Laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish: a multicentre case-control study [J]. Lancet, 2004,363(9425):1941-1947.

Lau SK, Lee LC, Fan RY, et al. Isolation of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, from Chinese tiger frog [J]. Int Journal Food Microbiol, 2009,129 (1):78-82.

Feng JL, Yan H, Chowdhury N, et al. Identification and characterization of integron-associated antibiotic resistant Laribacter hongkongensis isolated from aquatic products in China [J]. Int Journal Food Microbiol, 2011,144 (3):337-341.

张艳彪,程锦泉,郝元涛,等. 深圳市流动人口与户籍居民乙肝感染情况比较分析 [J]. 中华疾病控制杂志, 2012,16(5):424-427.

Woo PC, Lau SK, Tse H, et al. The Complete Genome and Proteome of Laribacter hongkongensis Reveal Potential Mechanisms for Adaptations to Different Temperatures and Habitats [J]. PLoS Genet, 2009,5(3):e1000416.

胡静,朱江峰,郑南才,等. 广东省江门市淡水产品中香港海鸥菌的检测 [J]. 疾病监测, 2013,(7):532-535.

Ni XP, Ren SH, Sun JR, et al. Laribacter hongkongensis Isolated from a Patient with Community-Acquired Gastroenteritis in Hangzhou City [J]. J Clin Microbiol, 2007,45(1):255-256.

朱江峰,郑南才,廖如燕,等. 广东省香港海鸥菌感染性腹泻1例分析 [J]. 中国公共卫生, 2011,27(4):403-405.

邱亚群,扈庆华,石晓路,等. 香港海鸥形菌分型方法建立与病原学分布调查 [J]. 实用预防医学, 2011,18(10):1830-1833.

Lau SK, Woo PC, Fan RY, et al. Seasonal and tissue distribution of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, in retail freshwater fish in Hong Kong [J]. Int J Food Microbiol, 2007,113(1):62-66.

Feng JL, Hu J, Lin JY, et al. The prevalence, antimicrobial resistance and PFGE profiles of Laribacter hongkongensis in retail freshwater fish and edible frogs of southern China [J]. Food Microbiol, 2012,32(1):118-123.

Teng JL, Woo PC, Ma SS, et al. Ecoepidemiology of Laribacter hongkongensis, a Novel Bacterium Associated with Gastroenteritis [J]. J Clin Microbiol, 2005,43(2):919-922.

Zhang Q, Ying G, Pan C, et al. Comprehensive Evaluation of Antibiotics Emission and Fate in the River Basins of China: Source Analysis, Multimedia Modeling, and Linkage to Bacterial Resistance [J]. Environ Sci Technol, 2015,49(11):6772-6782.

刘谢,柯碧霞,何冬梅,等. 2007-2013年广东省肠炎沙门菌耐药情况分析 [J]. 中华疾病控制杂志, 2015,19(3):269-272.

Relative Articles

Supplements(0)

Cited By

Proportional views