The interaction effect of renin gene MboI polymorphism and environmental factors in essential hypertension

Objective To explore interation effect of REN MboI polymorphism and environmental factors on essential hypertension. Methods A case-control study was performed in this study. Subjects (case:1 533, control:1 557) were from a field survey in the rural of Lanxi county of Heilongjiang province. The polymorphism was mainly genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In addition, in order to make sure the accuracy of PCR-RFLP, 10% PCR products were randomly selected to be sequenced. Results There were positive multiplication model of interaction between MboI polymorphism and sex, family history of hypertension, high density lipoprotein(HDL), the odds ratio of interaction (OR_i)were 1.36,1.12 and 1.03, respectively; There were negative multiplication model of interaction between MboI polymorphism and age, abdomen circumference(AC), body mass index(BMI), triglyceride (TG), with OR_i of 0.82,0.47,0.46 and 0.70 respectively. Conclusions When male or having family history of hypertension or high level of HDL is accompanied with mutation of MboI locus, the risk for hypertension will increase; when high level of age or AC or BMI or TG is accompanied with mutation of MboI locus, the risk for hypertension will decline.