Relationship between the expression of DcR3 and high-risk human papilloma virus infection in cervical carcinoma

HAN Jing; MIAO Hui; FENG Xiang-xian

doi:10.16462/j.cnki.zhjbkz.2019.01.018

Volume 23 Issue 1

Jan. 2019

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HAN Jing, MIAO Hui, FENG Xiang-xian. Relationship between the expression of DcR3 and high-risk human papilloma virus infection in cervical carcinoma[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2019, 23(1): 85-89. doi: 10.16462/j.cnki.zhjbkz.2019.01.018

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HAN Jing, MIAO Hui, FENG Xiang-xian. Relationship between the expression of DcR3 and high-risk human papilloma virus infection in cervical carcinoma[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2019, 23(1): 85-89. doi: 10.16462/j.cnki.zhjbkz.2019.01.018

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Relationship between the expression of DcR3 and high-risk human papilloma virus infection in cervical carcinoma

doi: 10.16462/j.cnki.zhjbkz.2019.01.018

1.
Department of Reproduction Heping Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China
2.
Department of Preventive Medicine, Changzhi Medical College, Changzhi 046000, China

Funds:

Major Scientific and Technological Subprojects of the 11th Five-Year Plan of the Ministry of Health 200902002-7

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Corresponding author: FENG Xiang-xian, E-mail: xfeng66@163.com
Received Date: 2018-07-10
Rev Recd Date: 2018-09-30
Publish Date: 2019-01-10

Abstract

Abstract

Objective To explore the relationship between the expression of decoy receptor 3 (DcR3) and high-risk human papilloma virus(HR-HPV) infection in cervical carcinoma. Methods Immunohistochemistry and hybird capture Ⅱ assay were used to detect the expression of DcR3 and HR-HPV in 35 cases of normal cervical tissues(NCE), 39 cases of cervical intraepithelial neoplasm(CIN) and 44 cases of cervical squamous epithelial carcinoma(CSES). Specific HR-HPV16 E7 siRNA and nonspecific HR-HPV16 E7 siRNA were synthesized and transfected to SiHa cells by Lipofectamine. The expression of DcR3 at mRNA and protein levels was examined by real-time polymerase chain reaction and western blot. The growth inhibition was examined by MTT assay. Results In NCE, CIN and CSES, the positive expression rates of DcR3 were 8.6%(3/35), 48.7%(19/39) and 77.3%(34/44), respectively, and the expression intensity was increasing(χ²=36.942, P<0.001). In NCE, CIN and CSES, the infection rates of HR-HPV were 5.7%(2/35), 56.4%(22/39) and 93.2%(41/44), respectively(χ²=60.322, P<0.001). The protein expression of DcR3 was positively correlated with the infection of HR-HPV in CSES(r=0.893, P=0.004). Conclusions DcR3 was highly expressed in cervical cancer. Its expression was positively correlated with HR-HPV infection, which may contribute to the occurrence and development of cervical cancer. HR-HPV silencing inhibited cellular growth and proliferation by down-regulating the expression of DcR3.
- Cervical carcinoma,
- DcR3,
- Human papilloma virus

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References(16)

References

[1]	Pelkofski E, Stine J, Wages NA, et al. Cervical cancer inwomen aged 35 years and younger[J]. Clin Ther, 2016, 38(3): 459-466. DOI: 10.1016/j.clinthera.2016.01.024.
[2]	Ryu HK, Baek JS, Kang WD, et al. The prognostic value of squamous cell carcinoma antigen for predicting tumor recurrence in cervical squamous cell carcinoma patients[J]. Obstet Gynecol Sci, 2015, 58(5): 368-376. DOI: 10.5468/ogs.2015.58.5.368.
[3]	周晖, 刘昀昀, 林仲秋. 宫颈癌二级预防: 2016年美国临床肿瘤协会资源分层临床实践指南解读[J]. 中国实用妇科与产科杂志, 2017, 33(2): 164-170. DOI: 10.19538/j.fk2017020110. Zhou H, Liu YY, Lin ZQ. Secondary prevention of cervical cancer: interpretation of the 2016 American society of clinical oncology resource stratification clinical practice guidelines[J]. Chin J Prac Gyne Obst, 2017, 33(2): 164-170. DOI: 10.19538/j.fk2017020110.
[4]	李军, 何宝明, 南星, 等. 陕西省汉中地区妇女人群HPV感染状况及不同年龄段亚型分布特征[J]. 中华疾病控制杂志, 2017, 21(7): 718-722. DOI: 10.16462/j.cnki.zhjbkz.2017.07.017. Li J, He BM, Nan X, et al. Investigation on HPV infection status and subtype distribution in women of different ages in Hanzhong area of Shanxi Province[J]. Chin J Dis Control Prev, 2017, 21(7): 718-722. DOI: 10.16462/j.cnki.zhjbkz.2017.07.017.
[5]	单玮, 张涛, 张铁军, 等. 我国女性人乳头瘤病毒(HPV)感染的流行病学现状[J]. 中华疾病控制杂志, 2017, 21(1): 89-93. DOI: 10.16462/j.cnki.zhjbkz.2017.01.021. Shan W, Zhang T, Zhang TJ, et al. The epidemiological situation of human papillomavirus infection among women in China[J]. Chin J Dis Control Prev, 2017, 21(1): 89-93. DOI: 10.16462/j.cnki.zhjbkz.2017.01.021.
[6]	Xiu Z, Shen H, Tian Y, et al. Serum and synovial fluid levels of tumor necrosis factor-like ligand 1A and decoy receptor 3 in rheumatoid arthritis[J]. Cytokine, 2015, 72(2): 185-189. DOI: 10.1016/j.cyto.2014.12.026.
[7]	Zhang X, Zhang Y, Xu J, et al. Antigen presentation of the Oct4 and Sox2 peptides by CD154-activated B lymphocytes enhances the killing effect of cytotoxic T lymphocytes on tumor stem-like cells derived from cisplatin-resistant lung cancer cells[J]. J Cancer, 2018, 9: 367-374. DOI: 10.7150/jca.20821.
[8]	Tong J, Ao R, Wang Y, et al. Prognostic and clinicopathological differences of DcR3 in gastrointestinal cancer: evidence from meta-analysis[J]. Int J Clin Exp Med, 2014, 7(9): 3096-3105.
[9]	Liang C, Xu Y, Li G, et al. Down regulation of DcR3 sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis[J]. Oncol Targets Ther, 2017, 10(2): 417-428. DOI: 10.2147/ott.s127202.
[10]	Ge H, Liang C, Ren S, et al. Prognostic value of DcR3 in solid tumors: A meta-analysis[J]. Clin Chim Acta, 2018, 481: 126-131. DOI: 10.1016/j.cca.2018.02.038.
[11]	Jiang M, Lin X, He R, et al. Decoyreceptor 3(DcR3) as a biomarker of tumor deterioration in femal reproductive cancers: a meta-analysis[J]. Med Sci Monit, 2016, 22: 1850-1857. DOI: 10.12659/msm.896226.
[12]	Weissinger D, Tagscherer KE, Macher-G ppinger S, et al. The soluble decoy receptor 3 is regulated by a PI3K-dependent mechanism and promotes migration and invasion in renal cell carcinoma[J]. Molecular Cancer, 2013, 12(1): 3365-3370. DOI: 10.1186/1476-4598-12-120.
[13]	杜玉晓, 王彩红, 王军, 等. DcR3蛋白在鼻咽癌组织中表达的临床意义及其与EB病毒关系的研究[J]. 中国实用医药, 2016, 11(20): 52-54. DOI: 10.14163/j.cnki.11-5547/r.2016.20.028. Du YX, Wang CH, Wang J, et al. Clinical significance of DcR3 protein expression in nasopharyngeal carcinoma and its relationship with EB virus[J]. China Prac Med, 2016, 11(20): 52-54. DOI: 10.14163/j.cnki.11-5547/r.2016.20.028.
[14]	Li L1, Xu C1, Long J, et al. E6 and E7 gene silencing results in decreased methylation of tumor suppressor genes and induces phenotype transformation of human cervical carcinoma cell lines[J]. Oncotarget, 2015, 6(27): 23930-23943. DOI: 10.18632/oncotarget.4525.
[15]	Zhou J, Peng C, Li B, et al. Transcriptional gene silencing of HPV16 E6/E7 induces growth inhibition via apoptosis in vitro and in vivo[J]. Gynecol Oncol, 2012, 124(2): 296-302. DOI: 10.1016/j.ygyno.2011.10.028.
[16]	王平, 刘珊, 程波, 等. siRNA介导的HPV16 E6/E7基因沉默对宫颈癌SiHa增殖及凋亡的影响[J]. 诊断病理学杂志, 2015, 22(12): 781-786. DOI: 10.3969/j.issn.1007-8096.2015.12.014. Wang P, Liu S, Cheng B, et al. Effects of siRNA-mediated HPV16 E6/E7 transcriptional gene stable silencing on proliferation and apoptosis of human cervical cancer SiHa cells[J]. Chin J Diagn Pathol, 2015, 22(12): 781-786. DOI: 10.3969/j.issn.1007-8096.2015.12.014.