Molecular characteristic of the two surface glycoproteins of highly pathogenic avian influenza A H5N6 viruses from human, 2014-2018, China

GU Hong-wei; QI Xian; CHEN Lei-yao; BAO Chang-jun

doi:10.16462/j.cnki.zhjbkz.2020.04.017

Volume 24 Issue 4

Jun. 2020

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GU Hong-wei, QI Xian, CHEN Lei-yao, BAO Chang-jun. Molecular characteristic of the two surface glycoproteins of highly pathogenic avian influenza A H5N6 viruses from human, 2014-2018, China[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(4): 456-461. doi: 10.16462/j.cnki.zhjbkz.2020.04.017

Citation:

GU Hong-wei, QI Xian, CHEN Lei-yao, BAO Chang-jun. Molecular characteristic of the two surface glycoproteins of highly pathogenic avian influenza A H5N6 viruses from human, 2014-2018, China[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(4): 456-461. doi: 10.16462/j.cnki.zhjbkz.2020.04.017

Citation:

GU Hong-wei, QI Xian, CHEN Lei-yao, BAO Chang-jun. Molecular characteristic of the two surface glycoproteins of highly pathogenic avian influenza A H5N6 viruses from human, 2014-2018, China[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(4): 456-461. doi: 10.16462/j.cnki.zhjbkz.2020.04.017

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Molecular characteristic of the two surface glycoproteins of highly pathogenic avian influenza A H5N6 viruses from human, 2014-2018, China

doi: 10.16462/j.cnki.zhjbkz.2020.04.017

GU Hong-wei¹,
QI Xian^{2
,
,},
CHEN Lei-yao^{1
,
,},
BAO Chang-jun²

1.
Nanjing Integrated Traditional Chinese and Western Medicine Hospital, Nanjing 210009, China
2.
Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China

Funds: Jiangsu Science & Technology Demonstration Project for Emerging Infectious Diseases Control and Prevention(BE2017749); Jiangsu Key Medical Discipline of Jiangsu Science & Technology Project(ZDXKA2016008)

More Information

Corresponding author: QI Xian, E-mail:qixiansyc@163.com; CHEN Lei-yao, E-mail:281252195@qq.com
Received Date: 2019-09-26
Rev Recd Date: 2019-12-23
Publish Date: 2020-04-10

Abstract

Abstract

Objective To analyze the molecular characteristic of hemagglutinin(HA) and neuraminidase(NA)proteins of highly pathogenic avian influenza H5 N6 viruses from 19 human cases. Methods Whole-genome sequences of original specimen and virus isolates were obtained by next-generation sequencing technology. Reference sequence information was collected from national center for biotechnology information(NCBI) and global initiative on sharing avian influenza data(GISAID) database. Pairwise sequence alignments and phylogenetic analysis were performed by Blasts, Clustal X 2.1 and Mega 6.1 softwares. Results A total of 23 cases of human infection with H5 N6 avian influenza virus occurred in China from 2014 to 2018. The HA and NA genes of H5 N6 virus from 19 cases were analyzed. HA evolutionary analysis showed that viruses belonged to Clade 2.3.4.4, of which 17 cases belonged to Group C; the first H5 N6 strain(A/Sichuan/26221/2014) belonged to Group D; and a Fujian case strain(A/Fujian-Sanyuan/21099/2017) belonged to Group B. The cleavage sites of HA protein of 29 strains of viruses in all 19 cases contained multiple basic amino acids. The 226-228 amino acids at the receptor binding sites of HA proteins of all viruses were QS(R) G(amino acids are sequenced according to the HA sequence of H3 subtype). In addition to one case in Guangzhou in 2014, the HA protein of 18 isolates mutated in T160 A, resulting in loss of glycosylation at 158 N site. In addition to A/Sichuan/26221/2014, 10 amino acids were deleted in 58-68 sites of NA proteins of strains from 18 cases. E627 K mutation occurred in 17 strains of virus PB2 protein from 9 cases. Conclusions From 2014 to 2018, the evolution of H5 N6 virus infection in China is active, and there is obvious genetic diversity. It is necessary to strengthen the monitoring of the evolution of H5 N6 viruses in China.
- Avian influenza virus,
- H5N6 subtype,
- Human cases,
- Molecular evolution

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References(22)

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