Rosiglitazone pretreatment alleviates renal oxidative stress in lipopolysaccharide-induced acute kidney injury

WEI Xiao; FU Lin; BO Qing-li; XU De-xiang; WU Yong-gui

doi:10.16462/j.cnki.zhjbkz.2020.05.019

Volume 24 Issue 5

Jul. 2020

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2020 > 24(5): 597-601

WEI Xiao, FU Lin, BO Qing-li, XU De-xiang, WU Yong-gui. Rosiglitazone pretreatment alleviates renal oxidative stress in lipopolysaccharide-induced acute kidney injury[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(5): 597-601. doi: 10.16462/j.cnki.zhjbkz.2020.05.019

Citation:

WEI Xiao, FU Lin, BO Qing-li, XU De-xiang, WU Yong-gui. Rosiglitazone pretreatment alleviates renal oxidative stress in lipopolysaccharide-induced acute kidney injury[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(5): 597-601. doi: 10.16462/j.cnki.zhjbkz.2020.05.019

Citation:

WEI Xiao, FU Lin, BO Qing-li, XU De-xiang, WU Yong-gui. Rosiglitazone pretreatment alleviates renal oxidative stress in lipopolysaccharide-induced acute kidney injury[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(5): 597-601. doi: 10.16462/j.cnki.zhjbkz.2020.05.019

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Rosiglitazone pretreatment alleviates renal oxidative stress in lipopolysaccharide-induced acute kidney injury

doi: 10.16462/j.cnki.zhjbkz.2020.05.019

1.
Center of Blood Purification, Department of Nephrology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, China
2.
Department of Toxicology, School of Public Health, Anhui Medical University, Hefei 230032, China
3.
Department of Nephrology, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, China

Funds: National Natural Science Foundation of China(81803268); Natural Science Foundation of Anhui Province(1808085MH257); Grants for BSKY of Anhui Medical University(XJ201820)

More Information

Corresponding author: WU Yong-gui, E-mail:wuyonggui@medmail.com.cn
Received Date: 2019-10-30
Rev Recd Date: 2020-01-20
Publish Date: 2020-05-10

Abstract

Abstract

Objective To investigate the effects of pretreatment with rosiglitazone(RSG) on oxidative stress in lipopolysaccharide(LPS)-induced acute kidney injury(AKI). Methods Forty-eight male ICR mice were divided into six groups: Control group, RSG group, LPS 6 h group, LPS 24 h group, RSG+LPS 6 h group, and RSG+LPS 24 h group. In the LPS and LPS+RSG groups, mice were intraperitoneal injected with LPS(2 mg/kg). In the RSG and RSG+LPS groups, mice were administered with RSG(10 mg/kg) by gavage four consecutive days before LPS injection. All mice were sacrificed at 6 h and 24 h after LPS injection. Some kidneys were collected for HE staining. Renal GSH and MDA were detected using biochemical method. Renal oxidant stress related genes were analyzed through real time RT-PCR. Renal NADPH oxidase-2 and NOX-4 were measured by Western blot. Serum was acquired for renal function. Results LPS-induced AKI, as determined renal pathological damage, renal function injury and inflammatory cell infiltration, were attenuated in RSG-pretreated mice. Although it did not affect renal GSH level, RSG pretreatment evidently inhibited the elevation of renal MDA content. Mechanistically, RSG pretreatment distinctly repressed LPS-induced upregulation of P47phox mRNA and nicotinamide adenine dinucleotide phosphate(NADPH) oxidase(NOX)-2 and NOX-4 in the kidney. Conclusion RSG pretreatment attenuates renal oxidative stress in LPS-induced acute kidney injury partially through inhibiting upregulation of NADPH oxidase.
- Rosiglitazone,
- Lipopolysaccharide,
- Acute kidney injury,
- Oxidative stress,
- NADPH oxidase

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References(15)

References

[1]	Mehta RL, Kellum JA, Shah SV, et al. Acute kidney injury network: report of an initiative to improve outcomes in acute kidney injury[J]. Crit Care, 2007, 11(2): R31.DOI: 10.1186/cc5713.
[2]	Sawhney S, Fraser SD. Epidemiology of AKI: utilizing large databases to determine the burden of AKI[J]. Adv Chronic Kidney Dis, 2017, 24(4): 194-204.DOI: 10.1053/j.ackd.2017.05.001.
[3]	Zager RA, Johnson AC, Lund S, et al. Acute renal failure: determinants and characteristics of the injury-induced hyperinflammatory response[J]. Am J Physiol Renal Physiol, 2006, 291(3): 546-556.DOI: 10.1152/ajprenal.00072.2006.
[4]	Heidari R, Ahmadi A, Mohammadi H, et al. Mitochondrial dysfunction and oxidative stress are involved in the mechanism of methotrexate-induced renal injury and electrolytes imbalance[J]. Biomed Pharmacother, 2018, 107: 834-840.DOI: 10.1016/j.biopha.2018.08.050.
[5]	Aladaileh SH, Hussein OE, Abukhalil MH, et al. Formononetin upregulates Nrf2/HO-1 signaling and prevents oxidative stress, inflammation, and kidney injury in methotrexate-induced rats[J]. Antioxidants(Basel), 2019, 8(10): 430.DOI: 10.3390/antiox8100430.
[6]	王春华, 俞百元, 余又新, 等.吴茱萸次碱对大鼠急性肾损伤氧化应激的影响及机制研究[J].安徽医科大学学报, 2018, 53(8): 1194-1197.DOI: 10.19405/j.cnki.issn1000-1492.2018.08.008. Wang CH, Yu BY, Yu YX, et al. Effects and mechanism of erigeronine on oxidative stress in rats with acute renal injury[J]. Acta Universitatis Medicinalis Anhui, 2018, 53(8): 1194-1197. DOI: 10.19405/j.cnki.issn1000-1492.2018.08.008.
[7]	Bo QL, Chen YH, Yu Z, et al. Rosiglitazone pretreatment protects against lipopolysaccharide-induced fetal demise through inhibiting placental inflammation[J]. Mol Cell Endocrinol, 2016, 423: 51-59.DOI: 10.1016/j.mce.2016.01.004.
[8]	Fu L, Chen YH, Bo QL, et al. Lipopolysaccharide downregulates 11β-hydroxysteroid dehydrogenase 2 expression through inhibiting peroxisome proliferator-activated receptor-γ in placental trophoblasts[J]. J Immunol, 2019, 203(5): 1198-1207.DOI: 10.4049/jimmunol.1900132.
[9]	Wang JX, Zhang C, Fu L, et al. Protective effect of rosiglitazone against acetaminophen-induced acute liver injury is associated with down-regulation of hepatic NADPH oxidases[J]. Toxicol Lett, 2017, 265: 38-46.DOI: 10.1016/j.toxlet.2016.11.012.
[10]	Xu S, Chen YH, Tan ZX, et al. Vitamin D3 pretreatment regulates renal inflammatory responses during lipopolysaccharide-induced acute kidney injury[J]. Sci Rep, 2015, 5: 186-187.DOI: 10.1038/srep18687.
[11]	Zhu JB, Xu S, Li J, et al. Farnesoid X receptor agonist obeticholic acid inhibits renal inflammation and oxidative stress during lipopolysaccharide-induced acute kidney injury[J]. Eur J Pharmacol, 2018, 838: 60-68.DOI: 10.1016/j.ejphar.2018.09.009.
[12]	El-Sayed SM, El-Naggar ME, Hussein J, et al. Effect of ficus carica L leaves extract loaded gold nanoparticles against cisplatin-induced acute kidney injury[J]. Colloids Surf B Biointerfaces, 2019, 184: 110465.DOI: 10.1016/j.colsurfb.2019.110465.
[13]	Muǹoz M, López-Oliva ME, Rodríguez C, et al. Differential contribution of Nox1, Nox2 and Nox4 to kidney vascular oxidative stress and endothelial dysfunction in obesity[J]. Redox Biol, 2019, 28: 101330.DOI: 10.1016/j.redox.2019.101330.
[14]	Simone S, Rascio F, Castellano G, et al. Complement-dependent NADPH oxidase enzyme activation in renal ischemia/reperfusion injury[J]. Free Radic Biol Med, 2014, 74: 263-273.DOI: 10.1016/j.freeradbiomed.2014.07.003.
[15]	Marcoux J, Man P, Petit-Haertlein I, et al. p47phox molecular activation for assembly of the neutrophil NADPH oxidase complex[J]. J Biol Chem, 2010, 285(37): 28980-28990.DOI: 10.1074/jbc.M110.139824.