The mechanism of CRISPR-mediated drug resistance and the relationship between the characteristics of CRISPR and isolation site in Klebsiella pneumoniae

CUI Cong-cong; LIANG Wen-juan; YANG Hai-yan; CHEN Shuai-yin; LONG Jin-zhao; DUAN Guang-cai

doi:10.16462/j.cnki.zhjbkz.2020.08.015

Volume 24 Issue 8

Aug. 2020

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CUI Cong-cong, LIANG Wen-juan, YANG Hai-yan, CHEN Shuai-yin, LONG Jin-zhao, DUAN Guang-cai. The mechanism of CRISPR-mediated drug resistance and the relationship between the characteristics of CRISPR and isolation site in Klebsiella pneumoniae[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(8): 939-945. doi: 10.16462/j.cnki.zhjbkz.2020.08.015

Citation:

CUI Cong-cong, LIANG Wen-juan, YANG Hai-yan, CHEN Shuai-yin, LONG Jin-zhao, DUAN Guang-cai. The mechanism of CRISPR-mediated drug resistance and the relationship between the characteristics of CRISPR and isolation site in Klebsiella pneumoniae[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(8): 939-945. doi: 10.16462/j.cnki.zhjbkz.2020.08.015

Citation:

CUI Cong-cong, LIANG Wen-juan, YANG Hai-yan, CHEN Shuai-yin, LONG Jin-zhao, DUAN Guang-cai. The mechanism of CRISPR-mediated drug resistance and the relationship between the characteristics of CRISPR and isolation site in Klebsiella pneumoniae[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2020, 24(8): 939-945. doi: 10.16462/j.cnki.zhjbkz.2020.08.015

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The mechanism of CRISPR-mediated drug resistance and the relationship between the characteristics of CRISPR and isolation site in Klebsiella pneumoniae

doi: 10.16462/j.cnki.zhjbkz.2020.08.015

School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453000, China
School of Public Health, Xinxiang Medical University, Xinxiang 453000, China
College of Public Health, Zhengzhou University, Zhengzhou 450000 , China

Funds:

National Science and Technology Specific Projects 2018ZX10301407

More Information

Corresponding author: DUAN Guang-cai, E-mail:gcduan@zzu.edu.cn
Received Date: 2020-03-13
Rev Recd Date: 2020-06-30
Publish Date: 2020-08-10

Abstract

Abstract

Objective To understand the regulatory mechanism of clustered regularly interspaced short palindromic repeats (CRISPR) to drug resistance of Klebsiella pneumoniae in respiratory specimens, and to analyze the relationship between the characteristics of CRISPR and the location of isolation. Methods 120 strains of Klebsiella pneumoniae were collected and genomic DNA was extracted. CRISPR-positive strains were identified by amplifying CRISPR/CRISPR-associated(Cas) related genes CRISPR 1 and CRISPR 2. The resistance phenotype of CRISPR-positive strains was detected by the BD Phoenix-100 bacterial identification instrument. CRISPR Target was used to look for homologous bacteriophages or plasmids with spacer sequence, find the drug resistance information of homologous bacteriophages or plasmids in Center for Genomic Epidemiology and detect the drug resistance genes of the strain where the spacer sequence is located, and analyze the relationship between the drug resistance genes of the two. CRISPR Finder was used to analyze CRISPR and multi-sequence alignment was used to analyze the consistency of spacer sequences. Results The positive rates of CRISPR 1 and CRISPR 2 were 12.50% and 13.33%; The homologous plasmids of the spacer sequence and the strains in which they were both carried common drug resistance genes, and the resistance phenotype of the strain was highly consistent with the drug resistance genes they carry. At the same location, the CRISPR distribution of the strains was extremely similar. Conclusions Klebsiella pneumoniae regulates drug resistance by integrating foreign plasmid resistance gene fragments into the strain's genome. The distribution of spacer sequence in CRISPR is closely related to the location of the strain isolation, which provides a theoretical basis for clinical treatment and infection control work.
- Klebsiella pneumoniae,
- CRISPR,
- Isolation location,
- Drug resistance

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References(23)

References

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