Characteristics and function analysis of vaginal microbiota in women with cervical cytology abnormalities under different HPV16 infection status

WANG Jia-hao; ZHANG Ming-xuan; ZHANG Le; SONG Li; DING Ling; PEI Rui-xin; YAN Jia-xin; WU Cai-hong; LYU Yuan-jing; CUI Meng; WANG Zhi-lian; WANG Jin-tao

doi:10.16462/j.cnki.zhjbkz.2023.03.004

Volume 27 Issue 3

Mar. 2023

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Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2023 > 27(3): 268-273

WANG Jia-hao, ZHANG Ming-xuan, ZHANG Le, SONG Li, DING Ling, PEI Rui-xin, YAN Jia-xin, WU Cai-hong, LYU Yuan-jing, CUI Meng, WANG Zhi-lian, WANG Jin-tao. Characteristics and function analysis of vaginal microbiota in women with cervical cytology abnormalities under different HPV16 infection status[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(3): 268-273. doi: 10.16462/j.cnki.zhjbkz.2023.03.004

Citation:

WANG Jia-hao, ZHANG Ming-xuan, ZHANG Le, SONG Li, DING Ling, PEI Rui-xin, YAN Jia-xin, WU Cai-hong, LYU Yuan-jing, CUI Meng, WANG Zhi-lian, WANG Jin-tao. Characteristics and function analysis of vaginal microbiota in women with cervical cytology abnormalities under different HPV16 infection status[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(3): 268-273. doi: 10.16462/j.cnki.zhjbkz.2023.03.004

Citation:

WANG Jia-hao, ZHANG Ming-xuan, ZHANG Le, SONG Li, DING Ling, PEI Rui-xin, YAN Jia-xin, WU Cai-hong, LYU Yuan-jing, CUI Meng, WANG Zhi-lian, WANG Jin-tao. Characteristics and function analysis of vaginal microbiota in women with cervical cytology abnormalities under different HPV16 infection status[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2023, 27(3): 268-273. doi: 10.16462/j.cnki.zhjbkz.2023.03.004

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Characteristics and function analysis of vaginal microbiota in women with cervical cytology abnormalities under different HPV16 infection status

doi: 10.16462/j.cnki.zhjbkz.2023.03.004

1.
Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China
2.
Department of Obstetrics and Gynecology, Second Hospital of Shanxi Medical University, Taiyuan 030001, China

Funds:

National Natural Science Foundation of China 81872705

More Information

Corresponding author: WANG Jin-tao, E-mail: wangjt59@163.com
Received Date: 2022-05-24
Rev Recd Date: 2022-07-30

Available Online: 2023-04-04

Publish Date: 2023-03-10

Abstract

Abstract

Objective To explore the characteristics of vaginal microbiota in women with abnormal cervical cytology under different HPV16 infection status, and to predict the function of the vaginal microbiota. Methods A total of 132 women with abnormal cervical cytology by liquid-based thinprep cytologic test (TCT), who came from the gynecological clinic of Second Hospital of Shanxi Medical University during January to June 2018, were enrolled in this study. Flow-through Hybridization technology was used to determine HPV infection typing. The vaginal microbiota was detected by 16S rDNA sequencing. The characteristic of vaginal microbiota were described by bio informatics, and Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to analyze the function of the vaginal microbiota. Results The Simpson index in the HPV16 positive group (n=59) was lower than that in the negative(n=73) group (P=0.047), while the Shannon index was higher in the positive group (P=0.036). Non-metric multidimensional scaling (NMDS) based on Bray-Curtis dissimilarities showed that there was no significance in Beta diversity between the two groups (P=0.199). Lactobacillus was the dominant bacteria in women with abnormal cervical cytology and different HPV16 infection status, but the proportion of Lactobacillus decreased (57.50%vs.60.69%) and anaerobic bacteria such as Gardnerella (14.29%vs.13.66%), and Peptoniphilus (4.28%vs.2.99%) increased in the HPV16 positive group. The abundance of Lactobacillus, Enterococcus, Staphylococcus and so on in the negative group was higher than that in the positive group, while the abundance of anaerobic bacteria such as Gardnerella, Ureaplasma, Peptoniphilus, Prevotella and so on in the HPV16 positive group was higher than that in the negative group. Ureaplasma and Peptoniphilus were the characteristic bacteria in HPV16 positive women based on Linear discriminant analysis Effect Size (LEfSe) analysis. The functional analysis showed that the function of vaginal microbiota was mainly concentrated on metabolism. The vaginal microbiota function in the HPV16 positive group was mainly to destroy the vaginal microenvironment and promote cell carcinogenesis, while the negative group was mainly to metabolism and inhibit cancer. Conclusions Increased diversity and the composition variation of vaginal microbiota are closely related to HPV16 infection. Ureaplasma and Peptoniphilus might be considered as potential biomarkers for HPV16 infection. Vaginal microbiota under HPV16 infection might induce vaginal microenvironment disorder and promote cancer.
- Cervical cytology abnormalities,
- Human papilloma virus 16,
- Vaginal microbiota,
- 16S rDNA sequencing,
- Functional prediction

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References(19)

References

[1]	Sung H, Ferlay J, Siegel RL, et al. GLOBAL cancer statistics 2020: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249. DOI: 10.3322/caac.21660.
[2]	Stratton P, Battiwalla M, Tian X, et al. Immune response following quadrivalent human papillomavirus vaccination in women after hematopoietic allogeneic stem cell transplant: a nonrandomized clinical trial[J]. JAMA Oncol, 2020, 6(5): 696-705. DOI: 10.1001/jamaoncol.2019.6722.
[3]	Wong AK, Chan RC, Nichols WS, et al. Human papillomavirus (HPV) in atypical squamous cervical cytology: the Invader HPV test as a new screening assay[J]. J Clin Microbiol, 2008, 46(3): 869-875. DOI: 10.1128/JCM.01424-07.
[4]	Castle PE, Solomon D, Schiffman M, et al. Human papillomavirus type 16 infections and 2-year absolute risk of cervical precancer in women with equivocal or mild cytologic abnormalities[J]. J Natl Cancer Inst, 2005, 97(14): 1066-1071. DOI: 10.1093/jnci/dji186.
[5]	Kaambo E, Africa C, Chambuso R, et al. Vaginal microbiomes associated with aerobic vaginitis and bacterial vaginosis[J]. Front Public Health, 2018, 6: 78. DOI: 10.3389/fpubh.2018.00078.
[6]	Norenhag J, Du J, Olovsson M, et al. The vaginal microbiota, human papillomavirus and cervical dysplasia: a systematic review and network meta-analysis[J]. BJOG, 2020, 127(2): 171-180. DOI: 10.1111/1471-0528.15854.
[7]	Cohen PA, Jhingran A, Oaknin A, et al. Cervical cancer[J]. Lancet, 2019, 393(10167): 169-182. DOI: 10.1016/S0140-6736(18)32470-X.
[8]	李俐, 丁玲, 吕元婧, 等. 阴道微环境改变与HPV16感染在宫颈上皮内瘤变中的作用及其交互效应[J]. 中华流行病学杂志, 2018, 39(11): 1486-1490. DOI: 10.3760/cma.j.issn.0254-6450.2018.11.013. Li L, Ding L, Lyu YJ, et al. Interaction between vaginal micro-environment alterations and HPV16 infection in cervical intraepithelial neoplasia[J]. Chin J Epidemiol, 2018, 39(11): 1486-1490. DOI: 10.3760/cma.j.issn.0254-6450.2018.11.013.
[9]	Di Paola M, Sani C, Clemente AM, et al. Characterization of cervico-vaginal microbiota in women developing persistent high-risk Human Papillomavirus infection[J]. Sci Rep, 2017, 7(1): 10200. DOI: 10.1038/s41598-017-09842-6.
[10]	Mitra A, MacIntyre DA, Ntritsos G, et al. The vaginal microbiota associates with the regression of untreated cervical intraepithelial neoplasia 2 lesions[J]. Nat Commun, 2020, 11(1): 1999. DOI: 10.1038/s41467-020-15856-y.
[11]	Wei ZT, Chen HL, Wang CF, et al. Depiction of vaginal microbiota in women with high-risk human papillomavirus infection[J]. Front Public Health, 2020, 8: 587298. DOI: 10.3389/fpubh.2020.587298.
[12]	Brotman RM, Shardell MD, Gajer P, et al. Interplay between the temporal dynamics of the vaginal microbiota and human papillomavirus detection[J]. J Infect Dis, 2014, 210(11): 1723-1733. DOI: 10.1093/infdis/jiu330.
[13]	Santiago GL, Deschaght P, El Aila N, et al. Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase[J]. Am J Obstet Gynecol, 2011, 204(5): 450. e1-e450, e4507. DOI: 10.1016/j.ajog.2010.12.061.
[14]	Chen Y, Qiu X, Wang W, et al. Human papillomavirus infection and cervical intraepithelial neoplasia progression are associated with increased vaginal microbiome diversity in a Chinese cohort[J]. BMC Infect Dis, 2020, 20(1): 629. DOI: 10.1186/s12879-020-05324-9.
[15]	Robinson JW, Dando SJ, Nitsos I, et al. Ureaplasma parvum serovar 3 multiple banded antigen size variation after chronic intra-amniotic infection/colonization[J]. PLoS One, 2013, 8(4): e62746. DOI: 10.1371/journal.pone.0062746.
[16]	Moodley J, Constant D, Mwaka AD, et al. Mapping awareness of breast and cervical cancer risk factors, symptoms and lay beliefs in Uganda and South Africa[J]. PLoS One, 2020, 15(10): e0240788. DOI: 10.1371/journal.pone.0240788.
[17]	Farr Zuend C, Noël-Romas L, Hoger S, et al. Influence of dapivirine vaginal ring use on cervicovaginal immunity and functional microbiome in adolescent girls[J]. AIDS, 2021, 35(3): 369-380. DOI: 10.1097/QAD.0000000000002751.
[18]	Gonzalez PS, O'Prey J, Cardaci S, et al. Mannose impairs tumour growth and enhances chemotherapy[J]. Nature, 2018, 563(7733): 719-723. DOI: 10.1038/s41586-018-0729-3.
[19]	Loftus RM, Assmann N, Kedia-Mehta N, et al. Amino acid-dependent cMyc expression is essential for NK cell metabolic and functional responses in mice[J]. Nat Commun, 2018, 9(1): 2341. DOI: 10.1038/s41467-018-04719-2.