乙草胺对大鼠肝脏氧化性应激状态的影响

李龙雪; 郑晶; 徐艺华; 庚天琦; 张旸

doi:10.16462/j.cnki.zhjbkz.2016.01.014

乙草胺对大鼠肝脏氧化性应激状态的影响

doi: 10.16462/j.cnki.zhjbkz.2016.01.014

1. 哈尔滨医科大学公共卫生学院毒理教研室, 黑龙江哈尔滨 150081;
2. 黑龙江省疾病预防控制中心公共卫生监测所, 黑龙江哈尔滨 150030

基金项目:

国家自然科学基金（81072333）

详细信息

作者简介:
李龙雪(1988-),女,黑龙江哈尔滨人,在读硕士研究生。主要研究方向:除草剂对健康损害作用。

中图分类号: R349.4;R181.1
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- 被引次数: 0
出版历程
- 收稿日期: 2015-10-27
- 修回日期: 2015-11-20

Study on oxidative stress of liver in rats exposed to acetochlor

1. Department of Toxicology, School of Public Health, Harbin Medical University, Harbin 150081, China;
2. Department of Public Health Monitoring, Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China

摘要

摘要: 目的探讨乙草胺对大鼠肝脏氧化性应激状态的影响。方法 Wistar大鼠按体重随机分为乙草胺高剂量组（107.40 mg/kg）、低剂量组（17.18 mg/kg）和阴性对照组，采用喂饲法连续染毒。染毒12周和24周时，分别处死一批动物，采用酶标法和荧光法检测大鼠肝脏总抗氧化能力（total antioxidant capacity，T-AOC）及活性氧（reactive oxygen species，ROS）、氧化型谷胱甘肽（glutathione disulfide，GSSG）和还原型谷胱甘肽（glutathione，GSH）含量。结果染毒12周和24周，乙草胺高剂量组大鼠肝脏脏体比与对照组相比差异具有统计学意义（均有P<0.05）；染毒24周，高、低剂量组大鼠肝脏出现嗜酸性变，肝脏GSSG和ROS含量均高于对照组（均有P<0.05），高剂量组肝组织T-AOC和GSH含量均低于对照组（均有P<0.05）。染毒24周高、低剂量组大鼠肝脏谷胱甘肽氧化还原电位升高（均有P<0.05）。结论乙草胺能改变大鼠肝脏的氧化还原状态，进而引起肝脏氧化性应激，导致肝脏损伤。
- 肝 /
- 氧化性应激 /
- 氧化还原
Abstract: Objective To explore the effect of acetochlor on oxidative stress of livers in rats. Methods Wistar rats were randomly divided into low-dose group (17.18 mg/kg), high-dose group (107.40 mg/kg) and control group by weight. Rats were administered with acetochlor through continuous feeding and were sacrificed at the 12 w and 24 w after treatment, respectively. Spectrophotometric method and enzyme-linked immunosorbent assay were used to measure the content of total antioxidant capacity (T-AOC), reactive oxygen species (ROS), glutathione disulfide (GSSG) and glutathione (GSH) of livers. Results The ratios of liver weight and body weight in the high-dose group were all significantly different from those in the control group at the 12 w and 24 w(all P<0.05). At the 24 w, in high-dose and low-dose groups, the eosinophilic change of livers in rats was observed, and GSSG content and ROS level of livers were all higher than that in the control group (all P<0.05). Also the T-AOC level and GSH content of livers in high-dose group were all lower than those in the control group (all P<0.05). GSH redox potential of livers in rats increased significantly in high-dose group and low-dose group (all P<0.05), compared to the control. Conclusions Acetochlor could change the redox state of livers in rats and thus cause the oxidative stress, resulting in livers injury.
- Liver /
- Oxidative stress /
- Oxidation reduction

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