SARS-CoV、SARSL-CoV与其他冠状病毒的种系发生分析

朱静媛; 李庆波; 丁记者; 何剑锋; 徐德忠; 段广才

doi:10.16462/j.cnki.zhjbkz.2016.02.001

SARS-CoV、SARSL-CoV与其他冠状病毒的种系发生分析

doi: 10.16462/j.cnki.zhjbkz.2016.02.001

1. 郑州大学公共卫生学院环境卫生学教研室, 河南郑州 450001;
2. 广东省疾病预防控制中心传染病预防控制所, 广东广州 511430;
3. 第四军医大学预防系流行病学教研室, 陕西西安 710032;
4. 新乡医学院河南省分子诊断与医学检验技术协同创新中心, 河南新乡 453003

基金项目:

国家自然科学基金（81373059）

详细信息

作者简介:
朱静媛(1976-),女,河南商丘人,副教授,博士。主要研究方向:分子流行病学。

中图分类号: R394.3;R181
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- 被引次数: 0
出版历程
- 收稿日期: 2015-10-30
- 修回日期: 2015-12-04

Phylogenetic analysis of SARS-CoV, SARSL-CoV, and other coronavirus

1. Department of Environmental Hygiene, School of Public Health, Zhengzhou University, Zhengzhou 450001, China;
2. Prevention and Control of Infections Disease, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China;
3. Department of Epidemiology, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032, China;
4. Henan Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, China

摘要

摘要: 目的分析不同流行时段重症急性呼吸综合征冠状病毒（severe acute respiratory syndromes coronavirus, SARS-CoV）病毒株与重症急性呼吸综合征样冠状病毒（severe acute respiratory syndromes like coronavirus,SARSL-CoV）及其他冠状病毒的分子进化关系。方法从美国国立生物技术信息中心获取不同分离时段的SARS-CoV（9条）、所有SARSL-CoV（24条）、其他冠状病毒（26条）的全基因组序列，用ClustalX 2.1软件进行多重序列比对，用邻接法（neighbor-joining, NJ）MEGA 6.0软件构建系统进化树。结果在包含SARS-CoV、SARSL-CoV和其他冠状病毒基因组序列的不同数据集中，流行早、中期SARS-CoV与流行末期毒株的基因组序列均分别属于不同的分支；以流行早、中期SARS-CoV为根的有根树显示，流行末期SARS-CoV遗传距离最远，而与SARSL-CoV和其他冠状病毒遗传距离更接近。结论流行末期SARS-CoV与流行早、中期SARS-CoV遗传差异大，且可能存在逆向/返祖进化。
- SARS病毒 /
- 冠状病毒科 /
- 系统分析
Abstract: Objective To analyze the molecular evolution of severe acute respiratory syndromes coronavirus (SARS-CoV) genomic sequences in different epidemic phase and to determine their possible relationship with SARS-like CoV or other coronavirus. Methods Nine SARS-CoV genomic sequences derived from different epidemic phases, 24 SARS-like CoV and 26 other coronavirus genomic sequences were collected. Multiple sequence alignments were conducted by ClustalX 2.0 and the phylogenetic trees were constructed using MEGA 6.0 software through Neighbor-Joining (NJ) method. Results SARS-CoV genomic sequences derived from early and middle phases were attributed to one branch and those from late phase to another separated branch with far genomic distance in every phylogenetic trees. With root of SARS-CoV genomic sequences derived from early and middle phases, those derived from late phases were the furthest branch and closer to SARSL-CoV or other coronavirus genomic sequences. Conclusions SARS-CoV genomic sequences derived from late phase have great genetic differences compared with those derived from early and middle phases. It's suggested that reverse or atavistic evolution occurred during the late phase of SARS epidemic.
- SARS virus /
- Coronaviridae /
- Systems analysis

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参考文献(12)

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