霉酚酸对肝细胞自噬相关因子表达的影响

蒋俊俊; 苏锦明; 李彧; 陈晖; 梁冰玉; 黄颉刚; 潘沛江; 刘洁; 赵芳凝; 梁浩; 霍文哲; 叶力

doi:10.16462/j.cnki.zhjbkz.2016.03.008

霉酚酸对肝细胞自噬相关因子表达的影响

doi: 10.16462/j.cnki.zhjbkz.2016.03.008

1. 广西医科大学公共卫生学院, 广西艾滋病防治研究重点实验室, 广西南宁 530021;
2. 广西医科大学第一附属医院老年消化内科, 广西南宁 530021;
3. 武汉大学动物实验中心/ABSL-3实验室, 湖北武汉 430000

基金项目:

国家自然科学基金（81271851）；广西自然科学基金（2013GXNSFCB019004）；广西人社厅基金（人社厅函[2013]277号）

详细信息

作者简介:
蒋俊俊(1983-),男,广西桂林人,助理研究员,硕士。主要研究方向:艾滋病、丙肝流行病学;苏锦明(1988-),女,广西梧州人,在读硕士研究生。主要研究方向:艾滋病、丙肝流行病学。

中图分类号: R512.63
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出版历程
- 收稿日期: 2015-11-04
- 修回日期: 2016-01-02

The effect of mycophenolic acid on expression of autophagy-related genes in hepatocytes

1. Guangxi Medical University, Guangxi Key Laboratory of AIDS Prevention and Research & School of Public Health, Nanning 530021, China;
2. Geriatrics Digestion Department of Internal Medicine, the First Affiliated Hospital of GuangXi Medical University, Nanning 530021, China;
3. Wuhan University Center for Animal Experiment/ABSL-3 Lab, Wuhan 430000, China

摘要

摘要: 目的比较日本爆发性丙型肝炎病毒1型（hepatitis C virus Japanese fulminant hepatitis-1, HCV JFH-1）感染的Huh7细胞中细胞自噬因子在霉酚酸（mycophenolic acid,MPA）处理组与对照组的表达差异, 探讨MPA对肝细胞自噬相关因子的调节影响。方法用HCV JFH-1感染Huh7细胞，用5 μg/ml的霉酚酸处理细胞24 h，用实时荧光定量聚合酶链式反应（quantitative real-time polymerase chain reaction, qPCR）和蛋白免疫印迹法测定细胞内HCV、微管相关蛋白1轻链3（microtubule-associated-protein 1 light chain, MAP1-LC3B, 简写LC3B）、自噬相关因子3（autophagy-related gene 3, ATG3）和自噬相关因子7（autophagy-related gene 7, ATG7）的信使核糖核酸（messenger ribonucleic acid,mRNA）和蛋白相对表达量，比较上述因子在处理组和对照组之间的差异。结果在核糖核酸（ribonucleic acid,RNA）和蛋白水平上，MPA可以降低HCV在Huh7细胞中的表达并且降低自噬标记物LC3B mRNA和LC3B-II的蛋白质表达（均有P<0.001）；MPA可以使Huh7细胞中的自噬相关因子ATG3和ATG7的mRNA和蛋白相对表达量下降（P<0.001；P=0.003； P=0.010；P=0.002）。结论 MPA可以抑制HCV JFH-1在Huh7细胞中的复制和细胞自噬，并且可以下调自噬因子ATG3和ATG7的表达，推测MPA可能通过调节自噬关键因子表达来抑制细胞自噬从而拮抗HCV的复制。
- 霉酚酸 /
- 自噬 /
- 肝炎,丙型
Abstract: Objective To investigate the effect of mycophenolic acid (MPA) on hepatitis C virus (HCV) replkication and the expression of autophagy-related genes treated by MPA in HCV JFH-1 infected Huh7 cells. Methods Huh7 cells were infected with hepatitis C virus Japanese fulminant hepatitis -1(HCV JFH-1), and then treated with 5μg/ml MPA for 24h. The treated cells were collected to detect the expression of HCV, microtubule-associated protein 1 light chain(LC3B), autophagy-related gene 3(ATG3) and autophagy-related gene 7(ATG7) at messenger ribonucleic acid (mRNA) and protein levels by real-time quantitative PCR(qPCR) and Western blot assay respectively. Results MPA treatment decreased the expression of HCV and LC3B mRNA and LC3B-II protein (P<0.001, compared with control group), and reduced the expression of ATG3 and ATG7 at mRNA and protein levels (P<0.001, P=0.003; P=0.010, P=0.002 compared with control group). Conclusions MPA treatment could inhibit HCV replication and block autopahgy by down-regulating the expression of ATG3 and ATG7. These results suggest that MPA has a potent antiviral ability against HCV replication by blocking cellular autophagy.
- Mycophenolic acid /
- Autophagy /
- Hepatitis C

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参考文献(20)

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霉酚酸对肝细胞自噬相关因子表达的影响

doi: 10.16462/j.cnki.zhjbkz.2016.03.008

作者简介:
蒋俊俊(1983-),男,广西桂林人,助理研究员,硕士。主要研究方向:艾滋病、丙肝流行病学;苏锦明(1988-),女,广西梧州人,在读硕士研究生。主要研究方向:艾滋病、丙肝流行病学。

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The effect of mycophenolic acid on expression of autophagy-related genes in hepatocytes

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留言板

霉酚酸对肝细胞自噬相关因子表达的影响

doi: 10.16462/j.cnki.zhjbkz.2016.03.008

作者简介: 蒋俊俊(1983-),男,广西桂林人,助理研究员,硕士。主要研究方向:艾滋病、丙肝流行病学;苏锦明(1988-),女,广西梧州人,在读硕士研究生。主要研究方向:艾滋病、丙肝流行病学。

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出版历程

The effect of mycophenolic acid on expression of autophagy-related genes in hepatocytes

计量

出版历程

目录

作者简介:
蒋俊俊(1983-),男,广西桂林人,助理研究员,硕士。主要研究方向:艾滋病、丙肝流行病学;苏锦明(1988-),女,广西梧州人,在读硕士研究生。主要研究方向:艾滋病、丙肝流行病学。