2014-2015年广州地区腹泻患儿和健康儿童致泻性大肠埃希菌流行特征及耐药分析

石挺丽; 黄建华; 李秀芬; 刘优招; 熊益权; 钟艳云; 邓淑云; 陈少威; 张婷; 钟雪珊; 冯梅; 陈清; 俞守义

doi:10.16462/j.cnki.zhjbkz.2016.04.002

文章导航 > 中华疾病控制杂志 > 2016 > 20(4): 329-332

石挺丽, 黄建华, 李秀芬, 刘优招, 熊益权, 钟艳云, 邓淑云, 陈少威, 张婷, 钟雪珊, 冯梅, 陈清, 俞守义. 2014-2015年广州地区腹泻患儿和健康儿童致泻性大肠埃希菌流行特征及耐药分析[J]. 中华疾病控制杂志, 2016, 20(4): 329-332. doi: 10.16462/j.cnki.zhjbkz.2016.04.002

引用本文:

SHI Ting-li, HUANG Jian-hua, LI Xiu-fen, LIU You-zhao, XIONG Yi-quan, ZHONG Yan-yun, DENG Shu-yun, CHEN Shao-wei, ZHANG Ting, ZHONG Xue-shan, FENG Mei, CHEN Qing, YU Shou-yi. Epidemiological characterization and antimicrobial resistance of diarrhea Escherichia coli from acute diarrheal and healthy children in Guangzhou City, 2014-2015[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(4): 329-332. doi: 10.16462/j.cnki.zhjbkz.2016.04.002

Citation:

SHI Ting-li, HUANG Jian-hua, LI Xiu-fen, LIU You-zhao, XIONG Yi-quan, ZHONG Yan-yun, DENG Shu-yun, CHEN Shao-wei, ZHANG Ting, ZHONG Xue-shan, FENG Mei, CHEN Qing, YU Shou-yi. Epidemiological characterization and antimicrobial resistance of diarrhea Escherichia coli from acute diarrheal and healthy children in Guangzhou City, 2014-2015[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(4): 329-332. doi: 10.16462/j.cnki.zhjbkz.2016.04.002

引用本文:

Citation:

SHI Ting-li, HUANG Jian-hua, LI Xiu-fen, LIU You-zhao, XIONG Yi-quan, ZHONG Yan-yun, DENG Shu-yun, CHEN Shao-wei, ZHANG Ting, ZHONG Xue-shan, FENG Mei, CHEN Qing, YU Shou-yi. Epidemiological characterization and antimicrobial resistance of diarrhea Escherichia coli from acute diarrheal and healthy children in Guangzhou City, 2014-2015[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2016, 20(4): 329-332. doi: 10.16462/j.cnki.zhjbkz.2016.04.002

2014-2015年广州地区腹泻患儿和健康儿童致泻性大肠埃希菌流行特征及耐药分析

doi: 10.16462/j.cnki.zhjbkz.2016.04.002

南方医科大学公共卫生与热带医学学院流行病学教研室, 广东广州 510515

详细信息

作者简介:
石挺丽(1992-),女,海南三亚人,在读硕士研究生。主要研究方向:分子流行病学。

中图分类号: R722.132;R181
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出版历程
- 收稿日期: 2015-11-14
- 修回日期: 2016-01-23

Epidemiological characterization and antimicrobial resistance of diarrhea Escherichia coli from acute diarrheal and healthy children in Guangzhou City, 2014-2015

Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China

摘要

摘要: 目的了解广州地区致泻性大肠埃希菌感染情况、流行病学特征及耐药情况。方法 2014年7月～2015年7月分别收集广州地区某综合医院腹泻患儿及健康儿童粪便标本143份、83份，进行细菌分离培养，采用聚合酶链反应检测肠致病性大肠埃希菌、聚集性大肠埃希菌、产毒性大肠埃希菌和肠侵袭性大肠埃希菌，Kirby Bauer法（K-B纸片扩散法）进行药敏试验。结果四种致泻性大肠埃希菌在腹泻患儿与健康儿童中的检出率分别为1.4%、3.6%；2.8%、2.4%；1.4%、1.2%；0.7%、1.2%；腹泻患儿3～7岁年龄段检出率最高，占26.7%；然而，健康儿童中5～7岁年龄段检出率最高，占8.4%。药敏试验结果显示，大肠埃希菌对四环素、氨苄西林耐药率为52.6%～63.2%；头孢类耐药率为5.3%～21.1%；多重耐药率50.5%。结论致泻性大肠埃希菌在腹泻患儿及健康儿童均有检出，并存在多重感染，提示致泻性大肠埃希菌是机会感染菌；除此之外，耐药及多重耐药高，应加强致泻性大肠埃希菌监测及管理抗生素使用。
- 腹泻 /
- 肠致病性大肠杆菌 /
- 流行病学方法
Abstract: Objective To investigate the epidemiological characteristics and antimicrobial resistance of diarrhea escherichia coli.Methods From June 2014 to June 2015, 143 and 83 stool samples were collected from children infected with acute diarrhea and healthy children respectively. Diarrhea escherichia coli was identified by biochemistry identification and PCR.Kirby Bauer methods(K-B disk diffusion methods) was adopted to evaluate antimicrobial resistance.Results The detection rates of enteropathogenic escherichia coli, enteroaggregative escherichia coli, enterotoxigenic escherichia coli and enteroinvasive escherichia coli in acute diarrhea children and health children specimens were 1.4% and 3.6%,2.8% and 2.4%,1.4% and 1.2%,0.7% and 1.2%,respectively. The age of 3-7 children with acute diarrhea had the highest detection rate of 26.7%, however,the age of 5-7 healthy children had the highest rate of 8.4%. The antimicrobial susceptibility tests showed that the resistance rates for tetracycline, ampicillin and cephalosporin were 52.6%-63.2%,and 5.3%-21.1%. In addition, the multi-drug resistance rate was 50.5%.Conclusions Diarrhea escherichia coli was detected in both acute diarrhea children and health children, and there were multiple infections,which hint diarrhea escherichia coli is an opportunistic infections bacteria. The result of antimicrobial resistance as well as multiple antimicrobial resistance are not optimistic. It is necessary to strengthen the surveillance of diarrheagenic escherichia coli and antimicrobial resistance, and providing a guide for clinical using antibiotic is also imperative.
- Diarrhea /
- Enteropathogenic escherichia coli /
- Epidemiologic methods

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