社区健康人生理性功能衰老和端粒长度分析

邓鹏飞; 周璇; 龙耀; 曾妮; 林菲; 仲瑷玲; 黄河浪

doi:10.16462/j.cnki.zhjbkz.2016.07.006

社区健康人生理性功能衰老和端粒长度分析

doi: 10.16462/j.cnki.zhjbkz.2016.07.006

邓鹏飞¹,
周璇¹,
龙耀¹,
曾妮¹,
林菲¹,
仲瑷玲²,
黄河浪^1, ,

1. 南昌大学研究生院流行病与卫生统计学教研室, 江西南昌 330006;
2. 复旦大学附属肿瘤医院检验科, 上海 200433

基金项目:

国家自然科学基金（81360446）

详细信息

作者简介:
邓鹏飞(1989-),男,江西赣州人,在读硕士研究生。主要研究方向:老年医学。

中图分类号: R339.38;R181.2
计量
- 文章访问数: 277
- HTML全文浏览量: 70
- PDF下载量: 33
- 被引次数: 0
出版历程
- 收稿日期: 2015-10-11
- 修回日期: 2016-02-29

Study on the evaluation of the physiological aging and detection of telomere length in healthy community people

1. Department of Epidemiology and Health Statistics, Graduate College, Nanchang University, Nanchang 330006, China;
2. Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai 200433, China

摘要

摘要: 目的探索社区健康人生理性功能衰老随年龄的变化趋势及其与人外周血白细胞端粒长度的关系，为科学评价衰老、延缓衰老提供依据或参考。方法采取分层随机抽样方法对南昌市20岁以上的健康人进行问卷调查，通过实时荧光定量PCR技术对部分样本外周血细胞端粒长度进行测量。结果现场实证研究共发放问卷1 000份，有效回收986份（98.60%），男女比例为0.97：1，分布均匀（t=1.319, P=0.995），年龄与衰老生理分值成高度正相关（r=0.892,P<0.001）。被测对象平均相对端粒长度为（1.057±0.031），老、中、青三个年龄段差异具有统计学意义（F=586.930，P<0.001），端粒长度与年龄（r=-0.814，P<0.001），生理性衰老分值（r=-0.682，P<0.001）均呈负相关。结论通过我国老年医学专家论证建立的生理性衰老指标体系，反映了生理衰老总分及方面得分随年龄增大而升高，并与端粒长度呈负相关的趋势。
- 衰老 /
- 端粒 /
- 老年医学
Abstract: Objective To explore the change trends of telomere length in peripheral blood leukocyte with age and physiological aging in healthy community people and provide basic evidence and reference for aging evaluation. Methods Stratified random sampling was used to investigate healthy residents beyond 20 years old who lived in Nanchang. Quantitative real-time PCR technology was used to measure telomere length. Results A total of 1 000 questionnaires were distributed and 986 of them were valid after serious confirmation(98.60%). The ratio of male to female was 0.97:1. There was no significant difference in gender distribution (t=1.319, P=0.995). There was a positive correlation between aging scores and age(r=0.892, P<0.001).The result of telomere length showed that the average relative telomere length was(1.057±0.031).Telomere length of old, middle and young people were significantly different (F=586.930, P<0.001). Telomere length was negatively correlated with age(r=-0.814,P<0.001) and physiological aging scores(r=-0.682,P<0.001). Conclusions The established physiological aging index system which based on literature review and expert consultation showed that the score of physical aging and its three aspects increased with age and telomere length is negatively correlated with aging scores.
- Aging /
- Telomere /
- Geriatrics

HTML全文

参考文献(22)

葛亮,王桦,曾尔亢,等. 衰老相关基因研究进展 [J].中国老年学杂志, 2014,34(22):6529-6532.

蒋长好,刘正之,陈婷婷. 锻炼对老化和大脑衰退的影响 [J]. 现代预防医学, 2014,41(5):868-871.

Finkel T. Signal transduction by reactive oxygen species [J]. J Cell Biol, 2011,194(1):7-15.

唐欣然,黄耀华,王杨,等. 单组目标值试验样本量计算方法的比较研究 [J]. 中华疾病控制杂志, 2013,17(11):993-996.

樊瑾,于普林,李小鹰.中国健康老年人标准(2013)解读2——健康评估方法 [J]. 中华老年医学杂志, 2014,33(1):1-3.

高凌云,李国栋,童坦君. DNA印迹法和实时定量PCR法在端粒长度测定中的应用比较 [J]. 北京大学学报(医学版), 2013,45(2):297-302.

Paúl C, Ribeiro O, Teixeira L. Active ageing: an empirical approach to the WHO model [J]. Curr Gerontol and Geriatr Res, 2012:382972.

Lapham K, Kvale MN, Lin J, et al. Automated assay of telomere length measurement and informatics for 100,000 subjects in the genetic epidemiology research on adult health and aging (GERA) cohort [J]. Genetics, 2015,200(4):1061-1072.

López-Otín C, Blasco MA, Partridge L, et al. The hallmarks of aging [J]. Cell, 2013,153(6):1194-1217.

Kimura M, Mizuta C, Yamada Y, et al. Constructing an index of physical fitness age for Japanese elderly based on 7-year longitudinal data: sex differences in estimated physical fitness age [J]. Age(Dordr), 2012,34(1):203-214.

Zhao X, Zhu S, Jia X, et al. Constructing a waist circumference density index to predict biological age and evaluating the clinical significance of waist circumference density age [J]. Exp Gerontol, 2013,48(4):422-426.

Boland BS, Dong MH, Bettencourt R, et al. Association of serum bilirubin with aging and mortality [J]. J Clin Exp Hepatol, 2014,4(1):1-7.

Rasmussen LJ, Sander M, Wewer UM, et al. Aging, longevity and health [J]. Mech Ageing Dev, 2011,132(10): 522-532.

Mary A, Bourguignon M, Wens V, et al. Aging reduces experience-induced sensorimotor plasticity. A magnetoencephalographic study [J]. Neuroimage, 2015,104(1):59-68.

Cheng CH, Hsu WY, Lin YY. Effects of physiological aging on mismatch negativity: a meta-analysis [J]. Int J Psychophysiol, 2013,90(2):165-171.

Sanderson WC, Scherbov S. Measuring the speed of aging across population subgroups [J]. PLoS One, 2014,9(5):e96289.

Honig LS, Kang MS, Schupf N, et al. Association of shorter leukocyte telomere repeat length with dementia and mortality [J]. Arch Neurol, 2012,69(10):1332-1339.

Ye S, Shaffer JA, Kang MS, et al. Relation between leukocyte telomere length and incident coronary heart disease events (from the 1995 Canadian Nova Scotia Health Survey) [J]. Am J Cardiol, 2013,111(7):962-967.

Unryn BM, Cook LS, Raibowol KT. Paternal age is positively linked to telomere length of children [J]. Aging Cell, 2005,4(2):97-101.

Der G, Batty GD, Benzeval M, et al. Is telomere length a biomarker for aging: cross-sectional evidence from the west of Scotland? [J]. PloS One, 2012,7(9):e45166.

Finkel T. Signal transduction by reactive oxygen species [J]. J Cell biol, 2011,194(1):7-15.

黄俊杰,王彩冰,何显教,等. maFGF对衰老大鼠脑和肝组织ATP酶活力及抗氧化作用的研究 [J]. 现代预防医学, 2013,40(1):121-123.

施引文献

资源附件(0)

访问统计