2015年深圳地区疱疹性咽峡炎的病原学与流行病学特征分析

王玉洁; 杨洪; 姚相杰; 张海龙; 陈龙; 何雅青

doi:10.16462/j.cnki.zhjbkz.2016.08.002

2015年深圳地区疱疹性咽峡炎的病原学与流行病学特征分析

doi: 10.16462/j.cnki.zhjbkz.2016.08.002

王玉洁¹,
杨洪²,
姚相杰²,
张海龙²,
陈龙²,
何雅青^1,2, ,

1. 南方医科大学公共卫生与热带医学学院流行病学系, 广东广州 510515;
2. 深圳市疾病预防控制中心微生物检验科, 广东深圳 518055

基金项目:

国家自然科学基金（81371815）

详细信息

作者简介:
王玉洁(1989-),女,河南新乡人,在读硕士研究生。主要研究方向:肠道病毒。

中图分类号: R512.51;R512.92;R181
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出版历程
- 收稿日期: 2016-04-22
- 修回日期: 2016-06-07

The etiological and epidemiological characteristics of herpangina cases in Shenzhen City, 2015

1. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China;
2. Department of Microbiology, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China

摘要

摘要: 目的分析2015年监测的深圳地区疱疹性咽峡炎的病原学情况和流行病学特征，为疱疹性咽峡炎的预防和控制提供数据基础。方法 2015年共收集深圳地区70例疱疹性咽峡炎病例粪标本,采用荧光定量RT-PCR（real time PCR，RT-PCR）和RT-snPCR（real time transcription-seminested PCR，RT-snPCR）法对样本进行肠道病毒（enterovirus，EV）检测，并对主要的流行株进行基因亚型的鉴别和系统进化分析。结果在70例疱疹性咽峡炎患者中，EV阳性率为88.57%；阳性结果中共检测出6种病毒，其中，柯萨奇病毒A6（coxsackie virus A6，CA6）和柯萨奇病毒A2（coxsackie virus A2，CA2）的阳性标本最多，分别为22例和16例，且分别占总发病病例数的31.43%和22.86%；基于核酸序列的VP1区构建的系统进化树显示，CA6毒株的基因亚型均为B型，核苷酸和氨基酸同源性分别为96.2%～100%和97.3%～100%。CA2与香港地区CA2流行株的序列高度同源，其核苷酸同源性为91.1%～99.6%，氨基酸同源性为92.4%～100%。结论 2015年深圳地区流行的病原均属于A组肠道病毒，其中B型CA6 是引起疱疹性咽峡炎的主要流行病毒株。
- 疱疹性咽峡炎 /
- 肠道病毒A型,人 /
- 柯萨奇病毒感染
Abstract: Objective To analyze the etiological and epidemic characteristics of herpangina (HA) monitored in Shenzhen City in 2015, and to provide laboratory data for the epidemic control. Methods Seventy herpangina samples were collected in Shenzhen city in 2015. RT-PCR and RT-snPCR assays were performed to detect HEVs-postive specimens. Phylogenetic tree was contructed to identify the genotype of the predominant epidemic isolates. And the nucleotide sequence homology was analyzed among Shenzhen viral sequences and others viral sequences published in GenBank. Results According to the laboratory test results, 62 cases were enterovirus positive and the positive rate was 88.57%. Among these positive cases, six serotypes of EV including CA6, CA2, CA10, EV71, CA16 and CA5 were detected. CA6 and CA2 were the predominant serotype. A total of 22 and 16 cases of herpangina were infected by CA6 and CA2 which accouted for 31.43% and 22.86% of the totcucases respectively. Phylogenetic analysis showed that all CA6 strains determined in this study were belonged to B subgenotype. The nucleotide sequences identities of the VP1 gene among CA6 strains were 96.2%-100%. The homology of amino acid of the VP1 gene among CA6 strains was 97.3%-100%. The VP1 sequences of Shenzhen CA2 isolates shared high similarity with those of Hong Kong isolates. And their homologies of nucleotide and amino acid sequences were 91.1%-99.6% and 92.4%-100%, respectively. Conclusions A total of six different serotypes were detected in this study in which all six serotypes belonged to HEV-A species. And subgenotype B virus of CA6 may play an important role in the development of herpangina and it was the predominant etiologic agent of herpangina cases in this study.
- Herpangina /
- Enterovirus A, human /
- Coxsackievirus infections

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参考文献(21)

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