深圳市某区淡水产品中香港海鸥菌的检测及耐药特征分析

冯梅; 刘优招; 何雅青; 刘志华; 陈清; 胡静

doi:10.16462/j.cnki.zhjbkz.2016.08.003

深圳市某区淡水产品中香港海鸥菌的检测及耐药特征分析

doi: 10.16462/j.cnki.zhjbkz.2016.08.003

冯梅¹,
刘优招¹,
何雅青²,
刘志华³,
陈清¹,
胡静^1, ,

1. 南方医科大学公共卫生与热带医学学院流行病学系, 广东广州 510515;
2. 深圳市疾病预防控制中心微生物检验科, 广东深圳 518055;
3. 南方医科大学南方医院感染内科, 广东广州 510515

基金项目:

国家自然科学基金（81373052）

详细信息

作者简介:
冯梅(1990-),女,四川邻水人,在读硕士研究生。主要研究方向:感染流行病学。

中图分类号: R181.1
计量
- 文章访问数: 255
- HTML全文浏览量: 52
- PDF下载量: 35
- 被引次数: 0
出版历程
- 收稿日期: 2016-04-07
- 修回日期: 2016-06-28

Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City

1. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangdong 510515, China;
2. Microbiology Inspection Division, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China;
3. Department of Infectious Diseases, Nanfang Hospital, Guangdong 510515, China

摘要

摘要: 目的了解深圳市南山区淡水产品中香港海鸥菌的污染情况，并分析其耐药特征。方法于2015年4~9月采购该区1家水产批发市场、1家超市和3家农贸市场淡水产品样本共计347份，检样接种改良头孢哌酮麦康凯琼脂（cefoperazone macconkey agar, CMA）培养分离可疑菌株，经革兰染色、生化试验及16S rRNA扩增测序确认；阳性菌株用纸片扩散法试验19种抗生素耐药性。结果在347份淡水产品中检出香港海鸥菌108份，阳性率31.12%，其中草鱼阳性率20.44%，虎纹蛙阳性率50.82%。蛙株和鱼株药敏结果比较显示：鱼来源株对一、三类头孢菌素类抗生素耐药率高，蛙来源株对四环素类、喹诺酮类和磺胺类耐药率高。在38株多重耐药香港海鸥菌中，9株鱼分离株、29株蛙分离株分别表现为6种和20种耐药表型。结论深圳市该区淡水产品中存在不同程度的香港海鸥菌的污染且蛙来源的香港海鸥菌耐药模式更加复杂。
- 淡水 /
- 抗药性,微生物 /
- 聚合酶链反应
Abstract: Objective To investigate the contamination status of Laribacter hongkongensis in freshwater products in Nanshan district of Shenzhen city, and to analyze the antibiotic resistant characteristic. Methods 347 freshwater product samples from five different aquatic product markets were collected during April to September 2015 in Shenzhen city. All the samples were inoculated by using cefoperazone macconkey agar (CMA). Suspicious isolates were identified by gram staining, biochemical tests, amplification and sequencing of 16S rRNA. Antimicrobial resistance of L. hongkongensis to 19 antimicrobial agents was tested using disc-diffusion (K-B) method to determine the antibiotic susceptibility patterns. Results A total of 108 (31.12%)strains were isolated among 347 samples of aquatic product. The positive rates of grass carps and tiger frogs were 20.44% and 50.82%, respectively. Comparison of drug susceptibilities between fish-derived strains and frog-derived strains showed that the higher proportion of fish strains than frog strains were the first-generation and second-generation cephalosporins, while the higher discrepancies for frog strains were observed with tetracycline, quinolones and sulfonamides. Among 38 multiple drug-resistant strains of L. hongkongensis, nine fish strains and 29 frog strains were presented 6 and 20 different resistant phenotype, respectively. Conclusions There are different levels of the contamination of L. hongkongensis in aquatic products in Shenzhen. In addition, strains derived from frog have more complex drug-resistant pattern.
- Fresh water /
- Drug resistance, microbial /
- Polymerase chain reaction

HTML全文

参考文献(16)

Marcos LA, DuPont HL. Advances in defining etiology and new therapeutic approaches in acute diarrhea [J]. J Infect, 2007,55(5):385-393.

Yuen KY, Woo PC, Teng JL, et al. Laribacter hongkongensis gen. nov., sp. nov., a novel gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema [J]. J Clin Microbiol, 2001,39(12):4227-4232.

Woo PC, Lau SK, Teng JL, et al. Association of Laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish: a multicentre case-control study [J]. Lancet, 2004,363(9425):1941-1947.

Lau SK, Lee LC, Fan RY, et al. Isolation of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, from Chinese tiger frog [J]. Int Journal Food Microbiol, 2009,129 (1):78-82.

Feng JL, Yan H, Chowdhury N, et al. Identification and characterization of integron-associated antibiotic resistant Laribacter hongkongensis isolated from aquatic products in China [J]. Int Journal Food Microbiol, 2011,144 (3):337-341.

张艳彪,程锦泉,郝元涛,等. 深圳市流动人口与户籍居民乙肝感染情况比较分析 [J]. 中华疾病控制杂志, 2012,16(5):424-427.

Woo PC, Lau SK, Tse H, et al. The Complete Genome and Proteome of Laribacter hongkongensis Reveal Potential Mechanisms for Adaptations to Different Temperatures and Habitats [J]. PLoS Genet, 2009,5(3):e1000416.

胡静,朱江峰,郑南才,等. 广东省江门市淡水产品中香港海鸥菌的检测 [J]. 疾病监测, 2013,(7):532-535.

Ni XP, Ren SH, Sun JR, et al. Laribacter hongkongensis Isolated from a Patient with Community-Acquired Gastroenteritis in Hangzhou City [J]. J Clin Microbiol, 2007,45(1):255-256.

朱江峰,郑南才,廖如燕,等. 广东省香港海鸥菌感染性腹泻1例分析 [J]. 中国公共卫生, 2011,27(4):403-405.

邱亚群,扈庆华,石晓路,等. 香港海鸥形菌分型方法建立与病原学分布调查 [J]. 实用预防医学, 2011,18(10):1830-1833.

Lau SK, Woo PC, Fan RY, et al. Seasonal and tissue distribution of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, in retail freshwater fish in Hong Kong [J]. Int J Food Microbiol, 2007,113(1):62-66.

Feng JL, Hu J, Lin JY, et al. The prevalence, antimicrobial resistance and PFGE profiles of Laribacter hongkongensis in retail freshwater fish and edible frogs of southern China [J]. Food Microbiol, 2012,32(1):118-123.

Teng JL, Woo PC, Ma SS, et al. Ecoepidemiology of Laribacter hongkongensis, a Novel Bacterium Associated with Gastroenteritis [J]. J Clin Microbiol, 2005,43(2):919-922.

Zhang Q, Ying G, Pan C, et al. Comprehensive Evaluation of Antibiotics Emission and Fate in the River Basins of China: Source Analysis, Multimedia Modeling, and Linkage to Bacterial Resistance [J]. Environ Sci Technol, 2015,49(11):6772-6782.

刘谢,柯碧霞,何冬梅,等. 2007-2013年广东省肠炎沙门菌耐药情况分析 [J]. 中华疾病控制杂志, 2015,19(3):269-272.

施引文献

资源附件(0)

访问统计