深圳市某区淡水产品中香港海鸥菌的检测及耐药特征分析

冯梅; 刘优招; 何雅青; 刘志华; 陈清; 胡静

doi:10.16462/j.cnki.zhjbkz.2016.08.003

深圳市某区淡水产品中香港海鸥菌的检测及耐药特征分析

doi: 10.16462/j.cnki.zhjbkz.2016.08.003

冯梅¹,
刘优招¹,
何雅青²,
刘志华³,
陈清¹,
胡静^1, ,

1. 南方医科大学公共卫生与热带医学学院流行病学系, 广东广州 510515;
2. 深圳市疾病预防控制中心微生物检验科, 广东深圳 518055;
3. 南方医科大学南方医院感染内科, 广东广州 510515

基金项目:

国家自然科学基金（81373052）

详细信息

作者简介:
冯梅(1990-),女,四川邻水人,在读硕士研究生。主要研究方向:感染流行病学。

中图分类号: R181.1
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- 被引次数: 0
出版历程
- 收稿日期: 2016-04-07
- 修回日期: 2016-06-28

Detection and drug resistance characteristic of Laribacter hongkongensis in freshwater products in a district of Shenzhen City

1. Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangdong 510515, China;
2. Microbiology Inspection Division, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China;
3. Department of Infectious Diseases, Nanfang Hospital, Guangdong 510515, China

摘要

摘要: 目的了解深圳市南山区淡水产品中香港海鸥菌的污染情况，并分析其耐药特征。方法于2015年4~9月采购该区1家水产批发市场、1家超市和3家农贸市场淡水产品样本共计347份，检样接种改良头孢哌酮麦康凯琼脂（cefoperazone macconkey agar, CMA）培养分离可疑菌株，经革兰染色、生化试验及16S rRNA扩增测序确认；阳性菌株用纸片扩散法试验19种抗生素耐药性。结果在347份淡水产品中检出香港海鸥菌108份，阳性率31.12%，其中草鱼阳性率20.44%，虎纹蛙阳性率50.82%。蛙株和鱼株药敏结果比较显示：鱼来源株对一、三类头孢菌素类抗生素耐药率高，蛙来源株对四环素类、喹诺酮类和磺胺类耐药率高。在38株多重耐药香港海鸥菌中，9株鱼分离株、29株蛙分离株分别表现为6种和20种耐药表型。结论深圳市该区淡水产品中存在不同程度的香港海鸥菌的污染且蛙来源的香港海鸥菌耐药模式更加复杂。
- 淡水 /
- 抗药性,微生物 /
- 聚合酶链反应
Abstract: Objective To investigate the contamination status of Laribacter hongkongensis in freshwater products in Nanshan district of Shenzhen city, and to analyze the antibiotic resistant characteristic. Methods 347 freshwater product samples from five different aquatic product markets were collected during April to September 2015 in Shenzhen city. All the samples were inoculated by using cefoperazone macconkey agar (CMA). Suspicious isolates were identified by gram staining, biochemical tests, amplification and sequencing of 16S rRNA. Antimicrobial resistance of L. hongkongensis to 19 antimicrobial agents was tested using disc-diffusion (K-B) method to determine the antibiotic susceptibility patterns. Results A total of 108 (31.12%)strains were isolated among 347 samples of aquatic product. The positive rates of grass carps and tiger frogs were 20.44% and 50.82%, respectively. Comparison of drug susceptibilities between fish-derived strains and frog-derived strains showed that the higher proportion of fish strains than frog strains were the first-generation and second-generation cephalosporins, while the higher discrepancies for frog strains were observed with tetracycline, quinolones and sulfonamides. Among 38 multiple drug-resistant strains of L. hongkongensis, nine fish strains and 29 frog strains were presented 6 and 20 different resistant phenotype, respectively. Conclusions There are different levels of the contamination of L. hongkongensis in aquatic products in Shenzhen. In addition, strains derived from frog have more complex drug-resistant pattern.
- Fresh water /
- Drug resistance, microbial /
- Polymerase chain reaction

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