广东省人群多重耐药金葡菌的携带及耐药性分析

余海峰; 王晓霖; 樊燕平; 姚振江; 陈思东; 叶小华

doi:10.16462/j.cnki.zhjbkz.2016.10.019

广东省人群多重耐药金葡菌的携带及耐药性分析

doi: 10.16462/j.cnki.zhjbkz.2016.10.019

1. 佛山市顺德区勒流医院职业病科, 广东佛山 528322;
2. 广东药科大学公共卫生学院流统系, 广东广州 510310

基金项目:

广东省教育厅青年创新人才类项目（广东药学院创新强校工程资助，2014KQNCX138）；佛山市顺德区医学科研基金（2015A005）

详细信息

作者简介:
余海峰(1968-),男,广东顺德人,主管医师,学士。主要研究方向:职业卫生与传染病控制。

中图分类号: R12;R184
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出版历程
- 收稿日期: 2016-05-25
- 修回日期: 2016-08-25

Carriage rate and antibiotic resistance of multidrug-resistant S. aureus isolates among residents in Guangdong Province

1. Department of Occupation, Leliu Hospital of Shunde, Foshan 528322, China;
2. Department of Epidemiology and Health Statistics, School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China

摘要

摘要: 目的调查广东省部分人群（动物从业人员和一般人群）的多重耐药金葡菌（multidrug-resistant S. aureus，MDRSA）携带情况，并分析菌株的耐药性。方法对研究对象进行问卷调查和鼻拭子采样，对金葡菌进行药敏试验，分析方法采用χ²检验。结果动物从业人员中金葡菌、MDRSA、甲氧西林耐药金葡菌（methicillin-resistant S. aureus，MRSA）的携带率均高于对照人群（分别为13.3% vs 9.3%；9.5% vs 3.4%；7.0% vs 1.4%；均有P<0.01）。金葡菌对青霉素、克林霉素、四环素和红霉素的耐药率较高，MDRSA主要耐药模式是同时对克林霉素、红霉素、四环素耐药。除了青霉素外，动物从业人员金葡菌对各类抗生素的耐药率均高于对照人群；MRSA菌株对各类抗生素的耐药率均高于甲氧西林敏感金葡菌。结论本研究提示职业性动物接触可能导致从业人员感染耐药细菌。
- 葡萄球菌,金黄色 /
- 职业暴露 /
- 流行病学方法
Abstract: Objective To investigate the carriage rate and antibiotic resistance of multidrug-resistant S. aureus among animal-related workers and general population in Guangdong Province. Methods We surveyed the study population by face-to-face questionnaire and nasal swab sampling. All S. aureus isolates were tested for antibiotic susceptibility test, and the data were analyzed by chi-squared test. Results The carriage rates of S. aureus, multidrug-resistant S. aureus and methicillin-resistant S. aureus (MRSA) were higher in animal-related workers than in controls (13.3% vs 9.3%; 9.5% vs 3.4%; 7.0% vs 1.4%; all P<0.01). The rates of S. aureus isolates showed high levels of resistance to penicillin, clindamycin, tetracycline and erythromycin, and the dominant multidrug resistance pattern of multidrug-resistant S. aureus isolates were mainly resistant to clindamycin, erythromycin and tetracycline. S. aureus strains isolated from animal-related workers showed higher levels of resistances to antibiotics, except for penicillin, than those from general population. The antibiotic-resistant rate was higher in MRSA isolates than in methicillin-sensitive S. aureus isolates. Conclusions This study suggests that occupational animal contact may lead to the infection of drug resistant bacteria.
- Staphylococcus aureus /
- Occupational exposure /
- Epidemiologic methods

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参考文献(21)

李莎莎,刘艳艳,李家斌. 2009-2011年阜阳地区常见病原菌分布和耐药性分析[J]. 中华疾病控制杂志, 2014,18(5):394-397.

刘伟东,叶小华,姚振江,等. 动物密切接触者耐甲氧西林金黄色葡萄球菌携带风险研究[J]. 中华疾病控制杂志, 2015,19(5):484-487.

Datta R, Shah A, Huang SS, et al. High nasal burden of methicillin-resistant Staphylococcus aureus increases risk of invasive disease[J]. J Clin Microbiol, 2014,52(1):312-314.

Klevens RM, Morrison MA, Nadle J, et al. Invasive methicillin-resistant Staphylococcus aureus infections in the United States[J]. JAMA, 2007,298(15): 1763-1771.

Zhang K, Sparling J, Chow BL, et al. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci[J]. J Clin Microbiol, 2004,42(11):4947-4955.

Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance[J]. Clin Microbiol Infect, 2012,18(3):268-281.

Salgado CD, Farr BM, Calfee DP. Community-acquired methicillin-resistant Staphylococcus aureus: a meta-analysis of prevalence and risk factors[J]. Clin Infect Dis, 2003,36(2):131-139.

Yan X, Song Y, Yu X, et al. Factors associated with Staphylococcus aureus nasal carriage among healthy people in Northern China[J]. Clin Microbiol Infect, 2015, 21(2):157-162.

Ho PL, Chiu SS, Chan MY, et al. Molecular epidemiology and nasal carriage of Staphylococcus aureus and methicillin-resistant S. aureus among young children attending day care centers and kindergartens in Hong Kong[J]. J Infect, 2012,64(5):500-506.

Lee J, Sung JY, Kim YM, et al. Molecular characterization of methicillin-resistant Staphylococcus aureus obtained from the anterior nares of healthy Korean children attending daycare centers[J]. Int J Infect Dis, 2011, 15(8):e558-e563.

Fang HW, Chiang PH, Huang YC. Livestock-associated methicillin-resistant Staphylococcus aureus ST9 in pigs and related personnel in Taiwan[J]. PLoS One, 2014,9(2):e88826.

Boost M, Ho J, Guardabassi L, et al. Colonization of butchers with livestock-associated methicillin-resistant Staphylococcus aureus[J]. Zoonoses Public Health, 2013,60(8):572-576.

Gilbert MJ, Bos ME, Duim B, et al. Livestock-associated MRSA ST398 carriage in pig slaughterhouse workers related to quantitative environmental exposure[J]. Occup Environ Med, 2012,69(7):472-478.

Smith TC, Male MJ, Harper AL, et al. Methicillin-resistant Staphylococcus aureus (MRSA) strain ST398 is present in midwestern U.S. swine and swine workers[J]. PLoS One, 2009,4(1):e4258.

Leibler JH, Jordan JA, Brownstein K, et al. Staphylococcus aureus nasal carriage among beefpacking workers in a Midwestern United States Slaughterhouse[J]. PLoS One, 2016,11(2):e0148789.

Brennan GI, Abbott Y, Burns A, et al. The emergence and spread of multiple livestock associated clonal complex 398 methicillin-resistant and methicillin-susceptible staphylococcus aureus strains among animals and humans in the Republic of Ireland, 2010-2014[J]. PLoS One, 2016,11(2):e0149396.

Narvaez-Bravo C, Toufeer M, Weese SJ, et al. Prevalence of methicillin-resistant Staphylococcus aureus in Canadian commercial pork processing plants[J]. J Appl Microbiol, 2016,120(3):770-780.

Bisdorff B, Scholholter JL, Claussen K, et al. MRSA-ST398 in livestock farmers and neighbouring residents in a rural area in Germany[J]. Epidemiol Infect, 2012,140(10):1800-1808.

Vandendriessche S, Vanderhaeghen W, Soares FV, et al. Prevalence, risk factors and genetic diversity of methicillin-resistant Staphylococcus aureus carried by humans and animals across livestock production sectors[J]. J Antimicrob Chemother, 2013,68(7):1510-1516.

Mulders MN, Haenen AP, Geenen PL, et al. Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The Netherlands[J]. Epidemiol Infect, 2010,138(5):743-755.

Liu W, Liu Z, Yao Z, et al. The prevalence and influencing factors of methicillin-resistant Staphylococcus aureus carriage in people in contact with livestock: A systematic review[J]. AM J Infect Control, 2015,43(5):469-475.

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