氯乙烯亚慢性染毒对大鼠肝细胞周期及mir-21和mir-192表达的影响

梁洁; 胡君阳; 高怡; 田凤洁; 吕懿; 仇玉兰

doi:10.16462/j.cnki.zhjbkz.2016.10.023

氯乙烯亚慢性染毒对大鼠肝细胞周期及mir-21和mir-192表达的影响

doi: 10.16462/j.cnki.zhjbkz.2016.10.023

山西医科大学公共卫生学院卫生毒理学教研室, 山西太原 030001

基金项目:

山西省基础研究计划项目（2013011059-1）

详细信息

作者简介:
梁洁(1989-),女,山西平遥人,在读硕士研究生。主要研究方向:职业毒理学。

中图分类号: R329.2;R33;R996;R181
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出版历程
- 收稿日期: 2016-04-01
- 修回日期: 2016-07-21

Effects of vinyl chloride monomer on cell cycle and expression of mir-21 and mir-192 in liver of rat

Department of Toxicology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China

摘要

摘要: 目的探讨氯乙烯（vinyl chloride monomer，VCM）亚慢性染毒对大鼠肝细胞周期以及microRNA21（mir-21）和microRNA192（mir-192）表达量的影响。方法将32只健康斯普拉-道来（sprague dawley，SD）大鼠随机分为三个实验组（5 mg/kg组，25 mg/kg组和125 mg/kg组）与一个对照组（25 mg/kg清洁空气组），每组8只。采用腹腔注射进行VCM染毒，每周3次（隔日染毒）。染毒12周，处死大鼠并摘取肝组织。制备肝单细胞悬液，使用流式细胞技术检测G0/G1期、S期和G2/M期肝细胞所占百分比；提取肝细胞中的小片段RNA（<200 nt），采用实时荧光定量PCR技术检测大鼠肝细胞中mir-21和mir-192的表达量。结果 VCM染毒大鼠G0/G1期、S期和G2/M期肝细胞所占百分比在各剂量组间差异均无统计学意义（均有P>0.05）；125 mg/kg组S期细胞与对照组相比明显增加，差异有统计学意义（t=-4.363，P=0.024）。各剂量组之间mir-21相对表达量差异有统计学意义（H=16.064，P=0.001）；与对照组和5 mg/kg组相比，25 mg /kg组及125 mg/kg组mir-21相对表达量均降低，差异均有统计学意义（均有P<0.05）。各剂量组之间mir-192相对表达量差异有统计学意义（H=15.939，P=0.001）；与对照组和5 mg/kg组相比，25 mg/kg组及125 mg/kg组mir-192相对表达量均降低，差异均有统计学意义（均有P<0.05）。结论 VCM亚慢性染毒导致大鼠肝细胞S期比例增加，mir-21和mir-192表达下调，具体调节机制有待进一步研究。
- 氯乙烯 /
- 肝细胞 /
- 细胞周期 /
- 微RNAs
Abstract: Objective To explore the sub-chronic toxicity effects of vinyl chloride monomer (VCM) on cell cycle and the expression of cell cycle related microRNA 21(mir-21)and microRNA 192 (mir-192) of rat liver. Methods Thirty-two healthy sprague dawley (SD) rats were randomly divided into three VCM exposure groups (5 mg/kg, 25 mg/kg and 125 mg/kg) and a control group (25 mg/kg clean air). The rats were exposed by intraperitoneal injection three times a week (every other day) for three months. The flow cytometry was used to measure the percent of each phase(G0/G1, S, and G2/M). The mir-21 and mir-192 was extracted and then quantified using real-time fluorescent quantitative PCR. Results The percentage of each phase of cell cycle was not significantly different among four groups (all P>0.05). The proportion of S-phase cells in 125 mg/kg group was higher than the control group(t=-4.363,P=0.024). Besides, the expressions of mir-21 varied significantly among four groups (H=16.064, P=0.001) and, furthermore, decreased significantly in both 25 mg/kg and 125 mg/kg group when they were compared with control and 5 mg/kg group (all P<0.05). Meanwhile, the expressions of mir-192 also varied significantly (H=15.939, P=0.001), and decreased significantly in both 25 mg/kg and 125 mg/kg group, compared with control and 5 mg/kg group (all P<0.05). Conclusions VCM sub-chronic exposure induced the increase of S-phase cells and decrease of the expression of mir-21 and mir-192.
- Vinyl chloride /
- Hepatocytes /
- Cell cycle /
- MicroRNAs

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