摘要:
目的 通过检测阿片类毒品（opiates）对HIV感染者中干扰素α（interferon-α，IFN-α）、干扰素诱导基因56（interferon-stimulated gene 56，ISG56）、粘病毒抵抗蛋白A（myxovirus resistance protein A，MxA）表达水平的影响，初步探讨阿片类毒品对人类免疫缺陷病毒（human immunodeficiency virus，HIV）复制影响的机制。方法 研究对象选取美沙酮门诊、艾滋病自愿咨询检测门诊人群200名，分为4组：Opiate+HIV+组、Opiate+HIV-组、Opiate-HIV+组和对照组。面对面问卷调查并采集研究对象外周静脉血，分离外周血单核细胞，采用实时荧光定量PCR法、免疫印迹法、ELISA法等检测4组人群中IFN-α、ISG56、MxA的mRNA，蛋白表达水平，比较相关因子相对表达量的差异。结果 4组人群在年龄、性别、民族、户籍所在地、婚姻状况、文化程度及职业等人口学特征方面差异均无统计学意义（均有P>0.05）。无论是在基因还是蛋白水平上，HIV感染人群中毒品滥用者的IFN-α、ISG56和MxA的表达水平均低于非毒品滥用者，差异均有统计学意义（均有P<0.05）。结论 阿片类毒品可能抑制抗病毒效应因子IFN-α、ISG56和MxA的表达，从而促进HIV-1的复制。
Abstract:
Objective To detect the effect of opiate abuse on HIV replication and the possible mechanism by analyzing the expression ofinterferon-α(IFN-α), interferon stimulated gene 56 (ISG56) and myxovirus resistance protein A (MxA) in peripheral blood mononuclear cells(PBMCs) among human immunodeficiency virus (HIV) infected patients with or without opiates abuse. Methods A total of 200 subjects were randomly recruited from methadone treatment and voluntary HIV counselling and testing centers, and were divided into four groups:Opiate+HIV+, Opiate-HIV+, Opiate+HIV-, and Control. A questionnaire was used to collect data on demographic characteristics. Plasma and PBMCs were isolated from blood samples of the subjects. The expression of IFN-α, ISG56 and MxA level in PBMCs were analyzed by RT-PCR,western blot and ELISA. Results There were no significant differences in gender, age, nationality, domicile, marital status, education level and occupation, and other demographic characteristics among four groups (all P>0.05). Among HIV-1-infected subjects, RNA and protein level of IFN-α, ISG56 and MxA in Opiate+HIV+group were lower than those in Opiate-HIV+ group (all P<0.05). Conclusions Opiate abuse may suppress the antiviral responses of IFN-α, ISG56 and MxA to promote HIV-1 replication.
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