淮安市男男性行为人群感染HIV-1的分子特征研究

燕清丽; 杨鹏飞; 范炜; 邢亚东; 刘纯成; 郭宏雄; 何南江; 姚海波

doi:10.16462/j.cnki.zhjbkz.2016.12.011

淮安市男男性行为人群感染HIV-1的分子特征研究

doi: 10.16462/j.cnki.zhjbkz.2016.12.011

1. 淮安市疾病预防控制中心检验科, 江苏淮安 223001;
2. 江苏省疾病预防控制中心免疫规划所, 江苏南京 210009

基金项目:

淮安市科技计划项目（HAS2015019-5）；淮安市预防医学科研基金（hayf201504，hayf201505）

详细信息

作者简介:
燕清丽(1982-),女,山西介休人,助理研究员,硕士。主要研究方向:分子病毒学;杨鹏飞(1983-),男,山西阳泉人,助理研究员,硕士。主要研究方向:分子病毒学;范炜(1981-),男,江苏淮安人,工程师,硕士。主要研究方向:艾滋病检测研究。

中图分类号: R373.51;R394.113;R181.13
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出版历程
- 收稿日期: 2016-08-01
- 修回日期: 2016-10-21

Molecular characterization of HIV-1 infection among men who have sex with men in Huai'an

1. Department of Clinical Laboratory, Huai'an Center for Disease Control and Prevention, Huai'an 223001, China;
2. Department of Immunization Program, Jiangsu Center for Disease Prevention and Control, Nanjing 210009, China

摘要

摘要: 目的了解江苏省淮安市男男性行为（men who have sex with men，MSM）人群感染HIV-1的基因亚型分布和分子特征。方法以现住址为淮安的HIV-1确证感染者为研究对象，利用巢式逆转录聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）方法扩增env基因片段，测序并进行分子进化分析。结果淮安市MSM人群感染的HIV-1基因亚型有5种，分别是CRF01-AE（68.21%，118/173）、CRF07-BC（16.76%，29/173）、CRF08-BC（0.58%，1/173）、CRF55-01B（1.16%，2/173）及B亚型（13.29%，23/173）。系统进化分析显示淮安流行的毒株与云南、辽宁、广西、江苏及湖南分离的同源性高，在进化树上与对应亚型的参考株聚集成簇。流行的优势亚型CRF01-AE中共检测出58个单倍型，单多样性指数（0.999）和核苷酸的多样性指数（0.150）显示淮安市株具有较高的遗传多样性。结论淮安市MSM人群感染的HIV-1存在多样性，其中CRF55-01B为首次在淮安市MSM感染人群发现，CRF01-AE是淮安市MSM人群中感染的主要流行株。
- HIV-1 /
- 基因 /
- 分子结构
Abstract: Objective To gain more insights into genotype and evolutionary characteristics of HIV-1 infection among men who have sex with men (MSM) in Huai'an,Jiangsu. Methods Local confirmed cases in Huai'an were collected. HIV-1 env genes were amplified by nested reverse transcription polymerase chain reaction (RT-PCR)from (RNA) DNA, followed by sequencing and molecular evolutionary analysis. Results The distribution of HIV-1 infection genotypes among MSM were as follows:CRF01-AE(68.21%, 118/173),CRF07-BC(16.76%, 29/173),CRF08-BC(0.58%, 1/173),CRF55-01B(1.16%, 2/173) and B (13.29%, 23/173). Comparison of env gene sequences indicated that the HIV-1 strains circulating in Huai'an had high nucleotide identity with strains in Yunnan, Liaoning, Guangxi, Jiangsu and Hunan and clustering with their subtypes together on the phylogenetic tree. Overall, 58 haplotypes of CRF01-AE genotype were defined among individuals and CRF01-AE genotype of Huai'an isolates were equipped with a high haplotypic diversity index (0.999) and low nucleotide diversity index (0.150) also indicated the high genetic diversities. Conclusions HIV-1 genotypes are diverse in Huai'an. CRF55-01B was firstly detected among MSM in Huai'an. CRF01-AE is the dominant epidemic sub-type in MSM.
- HIV-1 /
- Gene /
- Molecular structure