Abstract: Objective To explore infection situation of human immunodeficiency virus among 400 commercially sexual clients from Liuzhou and factors targeting the use of condoms, and to provide references for further work. Methods A total of 400 clients from Liuzhou were randomly enrolled for anonymous questionnaires. Peripheral blood samples were collected for detection of antibodies of human immunodeficiency virus (HIV). Treponema pallidum and hepatitis C virus. Results All clients were recruited from low entertainment places The rate of whole AIDS knowledge awareness was 93.3%. The rate of not using condom during commercial sex in the past time and year were 5.2% and 13.5%, respectively. The main reason was "non-comfortable". Multivariate Logistic regression analysis showed that age(OR=0.434, 95% CI:0.261-0.721,P=0.001) and the accepted detection of HIV (OR=2.103, 95% CI:1.064-4.158,P=0.032) were protective factors of persistent condom use in commercial sex in the past year; the positive rate of antibodies for human immunodeficiency virus (HIV). Treponema pallidum and hepatitis C virus were 1.3%,3.8% and 0.5%, respectively. Conclusions Most of clients from Liuzhou city are elderly adults. Both the low use of condom with female sex worker and the high rate of HIV infection play potential risk roles in the transmission from high risk population to general population. Therefore, targeted measures need to be taken in order to prevent the spread of HIV.