Abstract: Objective To understand the human immunodeficiency virus (HIV) testing provision of community-based organizations (CBOs) in the 3rd round China-care program in Yunnan Province and provide suggestions for HIV testing promotion. Methods Data was collected by online questionnaires and analyzed with SPSS 19.0. Results 50 CBOs participated in the 3rd round China-care Program in Yunnan Province.100.0% (50/50) could provide referred HIV testing services. 64.0%(32/50) of them could provide HIV testing service, 81.3%(26/32) could provide blood HIV rapid testing services and 53.1%(17/32) could provide oral fluid HIV rapid testing services. All 32 CBOs received HIV rapid testing training. 40.0%(20/50) of CBOs could provide HIV rapid testing. The attitudes for HIV rapid testing services in different Health departments were significantly different (χ²=15.309, P=0.004). Conclusions It is important for CBOs to provide HIV rapid testing service and identify HIV infections. Thus, it is necessary to strength potentials of CBOs in providing HIV rapid testing service in the 3rd round China-care program in Yunnan Province. Providing HIV rapid testing service and behavioral intervention at the same time could extend coverage of HIV testing.