Preliminary study on the molecular epidemiology of mycobacterium tuberculosis strains from Guizhou in Western China
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摘要: 目的 分析结核分枝杆菌临床分离株的基因型特征，估计结核分枝杆菌近期在人群中传播的情况。方法 在肺结核报告发病率高的4个县级结核病门诊收集临床分离结核菌株的相关信息，应用RD105缺失基因和MIRU-VNTR基因分型技术分析结核分枝杆菌DNA多态性。结果 273株结核菌株中49.1%的菌株属于北京基因型，15 MIRU-VNTR位点组合进行分析，273株结核菌株共被分为262种不同的基因型，其中独特型251株，占91.9%，其余22株（8.1%）菌株属于11个不同的基因型，菌株成簇率为8.1%。结论 15位点组合用于本次分子流行病学研究，能准确反映贵州地区结核分枝杆菌分子流行病学特征，贵州省4个县结核分枝杆菌菌株呈现较高的多态性，其中8.1%的患者是由于近期本地区传播造成。Abstract: Objective To analyze the molecular characteristics and estimate the transmission of mycobacterium tuberculosis in the crowd. Methods M. tuberculosis strains were isolated from pulmonary tuberculosis patients in four countries, where the pulmonary tuberculosis report incidence rates were higher than the rest of the province. The strains were analyzed with the deletion of region of difference 105 and mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) methods. Results Of 273 strains, 49.1% were identified to be Beijing family isolates, and all strains were typed into 262 MIRU-VNTR genotypes, of which 251 strains (91.9%) were unique genotypes and the remaining 22 strains (8.1%) were typed into 11 genotypes. The clustering rate was 8.1%. Conclusions It is the first time to apply 15 MIRU-VNTR loci to genotype the M. tuberculosis strains of Guizhou Province. The results suggest that the strains are highly polymorphic and 8.1% of patients are due to the recent transmission.
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