武汉市耐多药结核分枝杆菌的MIRU-VNTR分子特征

段琼红; 陈聪; 王坚杰; 周美兰; 任易; 陈军

doi:10.16462/j.cnki.zhjbkz.2017.01.006

武汉市耐多药结核分枝杆菌的MIRU-VNTR分子特征

doi: 10.16462/j.cnki.zhjbkz.2017.01.006

段琼红¹,
陈聪²,
王坚杰²,
周美兰^2, ,,
任易³,
陈军³

1. 武汉市结核病防治所结核病防控科, 湖北武汉 430030;
2. 武汉市结核病防治所硚口结核病防控科, 湖北武汉 430030;
3. 武汉市结核病防治所检验科, 湖北武汉 430030

基金项目:

湖北省自然科学基金（2015CFB310）；湖北省卫计委项目（WJ2015MB163）；武汉市卫计委项目（WG14B10）；武汉市中青年医学骨干人才项目（[2014]77）；武汉市卫计委项目（WG15A04）

详细信息

作者简介:
段琼红(1971-),女,湖北房县人,副主任医师,博士。主要研究方向:结核病预防控制。

中图分类号: R378.911;R181.2
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出版历程
- 收稿日期: 2016-07-04
- 修回日期: 2016-10-23

Analysis on genetic diversity of multi-drug-resistant tuberculosis in Wuhan with MIRU-VNTR method

1. Department of Tuberculosis Control, Wuhan Institute for Tuberculosis Control, Wuhan 430030, China;
2. Department of Tuberculosis Control for Qiaokou District, Wuhan Institute for Tuberculosis Control, Wuhan 430030, China;
3. Department of Clinical Laboratory, Wuhan Institute for Tuberculosis Control, Wuhan 430030, China

摘要

摘要: 目的描述武汉市耐多药结核分枝杆菌基因型分布及成簇特征。方法选取15个结核分枝杆菌重复序列（mycobacterial interspersed repetitive unit，MIRU），对98株耐多药结核分枝杆菌进行多位点数目可变串联重复序列分析（mycobacterial interspersed repetitive unit-variable number tandem repeat typing，MIRU-VNTR）分型，计算差异遗传值、Hunter-Gaston指数（Hunter-Gaston discriminatory index，HGDI）、成簇率以及近期感染率最小估计值。结果 MIRU位点组合的多态性差异遗传值为0.960 4、HGDI为0.970 8，多态性和分辨指数最高的位点为Mtub 21及MIRU 26。共产生7个基因簇和65个独立基因型，成簇率为33.67%，近期感染率最小估计为26.53%。结论 15位点MIRU-VNTR分型法分辨指数较高，适合于武汉地区耐多药结核分枝杆菌的基因分型。尽管武汉市耐多药肺结核病的流行多半归因于内源性复燃，但仍有较高比例的近期传播。
- 分枝杆菌,结核 /
- 广泛耐药结核 /
- 基因型
Abstract: Objective To determine the genotype and cluster characteristics of multi-drug-resistant (MDR) mycobacterium tuberculosis in Wuhan City. Mehtods 98 mycobacterium tuberculosis isolates from MDR tuberculosis (MDR-TB) patients were genotyped by mycobacterial interspersed repetitive unit-variable number tandem repeat typing (MIRU-VNTR). Allelic diversity, Hunter-Gaston discriminatory index (HGDI), cumulative clustering rate and minimum infection rate estimation were calculated. Results The cumulative allelic diversity was 0.960 4. The discriminatory power was 0.970 8. Gene locus with the highest h and HGDI were Mtub21 and MIRU26 respectively. Seven clusters and 65 unique genotype were identified from 98 isolates, and the cluster rate was 33.67% and the least estimated proportion of recent transmission was 26.53%. Conclusions The 15 locus MIRU-VNTR method demonstrate relatively high discriminatory power and it is suitable for MDR mycobacterium tuberculosis isolates genotyping. Although the epidemic of MDR-TB in Wuhan is mainly due to reactivation, there is still a partial contribution from recent transmission.
- Mycobacterium tuberculosis /
- Extensively drug-resistant tuberculosis /
- Genotype