Abstract: Objective To determinate the seroprevalence associated with hepatitis E virus(HEV)exposition among HIV-infected people in Xiaogan City, which could provide the basic data better to prevent and control the epidemiology of HIV and hepatitis E virus. Methods 203 serum samples of HIV-infected people from May 2015 to September 2016 were collected. Demographic data, other disease histories and laboratory data for these subjects were collected from Chinese disease control information system. Serum samples were performed for anti-HEV IgG, anti-HEV IgM and HEV-Ag screening, in which liver function were screened among positive anti-HEV IgM subjects. Results For the 203 HIV-infected people, anti-HEV IgM positive ratio was 2.46%, anti-HEV IgG positive ratio was 43.31%(94/203). There was no significant difference in anti-HEV IgG positive rate between different gender(χ²=0,P=0.996). The anti-HEV IgG positive rate of subjects was as high as 80% in divorced group, but showed significant difference compared with another marital status groups(all P<0.05). Anti-HEV IgG positive rate for the crowd with CD4 value ≤ 200 cell/μl was higher than that of > 200 cell/μl, but showed no significant difference(χ²=0.286,P=0.593), and CD4 indexs of two positive anti-HEV IgM patients were 189 and 199 in cell/μl, respectively, followed with abnormal liver function in one patient. In addition, anti-HEV IgG positive rate in seven counties showed similar without difference(χ²=5.906,P=0.551). Conclusions There were higher HEV infection rate among HIV-infected people in Xiaogan City, thus, it is essential to positively carry out double-way screening for these two diseases at the same time, and take several targeted measures to prevent and treat them.