Abstract: Objective The application of spatial information technology and big data technology in the construction of dengue risk assessment system provides an information platform and scientific basis for the rapid assessment of disease risk. Methods The risk assessment system was developed by secondary development method, based on the hybrid programming language, and the software functions such as SaTScan, ENVI and ArcGIS. Results We successfully developed a set of risk assessment system of dengue fever based on spatial information technology and big data technology, and the overall design, function modules and key technologies of the system were introduced in detail. The main functions of the system included spatio-temporal aggregation detection, influencing factor extraction, risk assessment, big data analysis and visualization. Taking the outbreak of dengue fever in mainland China in 2014 as an application case, the risk assessment of dengue fever epidemic and the production of corresponding thematic products were realized from three levels of China, Guangdong and Guangzhou. Conclusions The establishment of the system provided a new information platform for the rapid assessment of dengue fever risk in typical areas of China, and improved the automation level of dengue fever monitoring and assessment.