Abstract: Objective To explore the prevalence of lower grade students' sub-health and correlative factors in medical colleges with the purpose to formulate reference data for popularizing healthy lifestyle and scientific intervention of sub-health among college students. Methods A total of 4 952 students were surveyed by Questionnaire, SPSS 21.0 software was used for statistical analysis. Results The sub-health score was 14(7,27), with a detection rate of 38.1%, and the healthy status of students from towns was better than those from rural areas(Z=-3.599, P<0.001). The common symptoms of sub-health among college students were fatigue (36.6%), low concentration (29.6%) and poor memory (28.1%), which was even worse especially among those with higher sub-health score. The scores of diet(Z=-11.264, P<0.001), physical activities(Z=-7.172, P<0.001) and adverse events(Z=-14.207, P<0.001) of healthy people were better than those of sub-health. The results of multiple Logistic regression analysis showed that 4 factors influenced the sub-health status among the students, including academic achievement (OR=1.647, 95% CI:1.149-2.363, P=0.007), body mass index(OR=1.117, 95% CI:1.006-1.240, P=0.038), adverse events (OR=1.470, 95% CI:1.154-1.874, P=0.002) and physical activities (OR=0.882, 95% CI:0.790-0.985, P=0.026). Conclusion Universities should pay more attention to the health state of college students as well as actively conduct targeted intervention activities.