有偿献血HCV高危人群IL-6水平与HCV RNA及肝功能的相关性研究

杨静静; 黄鹏; 田亭; 凡豪志; 喻荣彬; 岳明; 张云; 王洁

doi:10.16462/j.cnki.zhjbkz.2018.02.015

有偿献血HCV高危人群IL-6水平与HCV RNA及肝功能的相关性研究

doi: 10.16462/j.cnki.zhjbkz.2018.02.015

杨静静¹,
黄鹏²,
田亭²,
凡豪志²,
喻荣彬²,
岳明³,
张云^2,4, ,,
王洁^5, ,

1. 河海大学医院预防保健科, 江苏南京 210098;
2. 南京医科大学公共卫生学院流行病与卫生统计学系, 江苏南京 211166;
3. 南京医科大学第一附属医院感染病科, 江苏南京 210029;
4. 南京军区军事医学研究所流行病学室, 江苏南京 210002;
5. 南京医科大学护理学院基础与社区护理学系, 江苏南京 211166

基金项目:

国家自然科学基金（81473028，81473029，81502853）；江苏省自然科学基金（BK20151026）；江苏省重点学科建设项目“护理学”（JX10617801）；江苏高校“青蓝工程”

详细信息

作者简介:
杨静静(1980-),女,江苏盐城人,主治医师,硕士。主要研究方向:公共卫生传染性疾病控制。

中图分类号: R373.21;R392.114
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出版历程
- 收稿日期: 2017-07-18
- 修回日期: 2017-11-19

Study on correlation of the plasma IL-6 concentration with HCV RNA and liver function in HCV high-risk paid blood donors

1. Department of Prevention and Health, Hohai University Hospital, Nanjing 210098, China;
2. Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing 211166, China;
3. Department of Infectious Diseases, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;
4. Department of Epidemiology, Nanjing Military Medical Research Institute, Nanjing 210002, China;
5. Department of fundamental and Community Nursing, School of Nursing, Nanjing Medical University, Nanjing 211166, China

摘要

摘要: 目的了解有偿献血丙型肝炎病毒（hepatitis C virus，HCV）高危人群血浆白细胞介素6（interleukin 6，IL-6）水平与HCV RNA病毒载量及肝功能指标的相关性，探讨细胞因子在HCV感染慢性化机制中的作用。方法采用酶联免疫吸附法（enzyme-linked immunosorbent，ELISA）检测HCV持续感染者（138例）和对照组未感染者（152例）的血浆IL-6水平，采用荧光定量聚合酶链反应（fluorescence quantitative polymerase chain reaction，FQ-PCR）法检测其HCV RNA水平，采用Spearman相关和偏相关分析以上指标间的相关性。结果 HCV慢性感染者的血浆IL-6水平高于对照组（t=-3.066，P=0.002）；IL-6与丙氨酸氨基转移酶（alanine aminotransferase，ALT）和天门冬氨酸氨基转移酶（aspartate aminotransferase，AST）水平均呈正相关（调整前：r_ALT=0.186，P_ALT=0.001，r_AST=0.177，P_AST=0.003；调整后：r_ALT=0.200，P_ALT=0.001，r_AST=0.127，P_AST=0.039）；IL-6与HCV RNA水平呈正相关（调整前r=0.199，P=0.019；调整后r=0.200，P=0.025）。结论血浆IL-6可能与HCV慢性感染密切相关，宿主免疫在HCV感染的慢性化机制中可能发挥着不可忽视的作用。
- 丙型肝炎病毒 /
- 慢性病 /
- 白细胞介素6 /
- 病毒载量 /
- 丙氨酸氨基转移酶
Abstract: Objective To explore the relationship of plasma interleukin 6(IL-6) concentration with HCV RNA, liver function in hepatitis C patients and investigate the role of cytokines in chronic HCV infection. Methods 138 patients with persistent HCV infection and 152 uninfected controls were selected in this study. The plasma IL-6 level and HCV viral load were detected by enzyme linked immunosorbent assay (ELISA) and fluorescence quantitative polymerase chain reaction (FQ-PCR) respectively. The spearman correlation and partial correlation analyses were used to explore their correlations with liver function indexes. Results The chronic HCV infected patients had higher plasma IL-6 level than that of healthy controls (t=-3.066, P=0.002). The IL-6 concentration had a positive correlation with alanine aminotransferase (ALT), aspartate aminotransferase (AST) (before the adjustment:r_ALT=0.186, P_ALT=0.001, r_AST=0.177. P_AST=0.003; after the adjustment:r_ALT=0.200, P_ALT=0.001, r_AST=0.127, P_AST=0.039). The HCV viral load was positively correlated with the IL-6 level in chronic hepatitis C patients (before the adjustment:r=0.199, P=0.019; after the adjustment:r=0.200, P=0.025). Conclusions The plasma IL-6 may be associated with HCV infection and chronic level. The findings provided more insights into the immune mechanism for the development of HCV infection.
- Hepatitis C virus /
- Chronic disease /
- Interleukin-6 /
- Viral load /
- Alanine aminotransferase