Abstract: Objective To investigate the hand hygiene (HH) behavior of kindergarten teachers, and evaluate the intervention effects based on compliance. Methods Stratified cluster random sampling technique was performed in this study. 18 kindergartens were selected from two districts in Shenzhen city, and were then randomly assigned to either intervention group (12 kindergartens) or control group (6 kindergartens). Hand hygiene products, kick off activity, health education materials and health education session were main interventions. Teacher's HH compliance was observed before and one, three, and six months after the intervention, and self-reported questionnaires of HH compliance were completed before and after intervention. Then the intervention effectiveness was evaluated by comparing the HH compliance of intervention group and control group. Results Before the implementation of intervention, observed HH compliance of teachers was 10.4% (23/222), and the self-reported compliance was 9.4±1.5 (scale 0-10). Self-reported HH compliance of intervention group increased from 9.21 to 9.65 after the intervention (Z=-1.951, P=0.051),but the difference of control group had no statistical significance before and after the intervention (Z=-1.531, P=0.126). There was no signifiant difference in observed HH compliance between intervention group and control group before intervention (OR=0.43, 95% CI:0.18-1.02), and observed HH compliance in the intervention group was significantly higher after intervention than that in the control group (OR=11.53, 95% CI:5.71-23.30). Conclusions HH compliance of teachers increased due to the multiple interventions, so dissemination of intervention can be performed in kindergartens in Shenzhen, China.