图们江流域中朝俄边境地区长角血蜱中SFTS病毒监测分析

李基旭; SHIN-HyeongCho; 朴文; 金光俊; 宋振海; 全圣焕; 朴光明

doi:10.16462/j.cnki.zhjbkz.2019.02.016

图们江流域中朝俄边境地区长角血蜱中SFTS病毒监测分析

doi: 10.16462/j.cnki.zhjbkz.2019.02.016

1.
133001 延吉, 吉林省延边州疾病预防控制中心病媒科
2.
363951 韩国清州, 韩国疾病预防控制中心国立保健研究院疟疾与寄生虫科
3.
133300 珲春, 珲春市疾病预防控制中心副主任办公室
4.
133300 珲春, 珲春市疾病预防控制中心卫生监测科
5.
133100 图们, 图们市疾病预防控制中心病媒科
6.
133500 和龙, 和龙市疾病预防控制中心传染病科
7.
133400 龙井, 龙井市疾病预防控制中心病媒科

基金项目: 韩国疾病预防控制中心国立保健研究院国际感染性疾病检测体系援助项目

详细信息

通讯作者:
SHIN-Hyeong Cho, E-mail: cho4u@korea.kr

中图分类号: R512.99
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出版历程
- 收稿日期: 2018-04-15
- 修回日期: 2018-09-18
- 刊出日期: 2019-02-10

Surveillance and analysis of Haemaphysalis longicornis with SFTS virus in Tumen river basin located in the frontiers of Russia, Korea and Northeast China

1.
Division of Vector Control, Yanbian Center for Disease Control and Prevention Yanji, Yanbian 133001, China
2.
Division of Malaria and Parasites, National Research Institute of Health, South of Korea Center for Disease Control and Prevention, Cheongju 363951, Korea
3.
Deputy Director's Office, Hunchun Center for Disease Control and Prevention, Hunchun 133300, China
4.
Division of Health investigation, Hunchun Center for Disease Control and Prevention, Hunchun 133300, China
5.
Division of Vector Control, Tumen Center for Disease Control and Prevention, Tumen 133100, China
6.
Division of Infectious Disease Control, Helong Center for Disease Control and Prevention, Helong 133500, China
7.
Division of Vector Control, Longjing Center for Disease Control and Prevention, Longjing 133400, China

Funds: International Laboratory Installation and Collaborative Research on Neglected Tropical and Vector-borne Diseases, KCDC

More Information

Corresponding author: SHIN-Hyeong Cho, E-mail: cho4u@korea.kr

摘要

摘要: 目的掌握图们江流域中朝俄边境地区长角血蜱分布特征，了解该地区长角血蜱携带发热伴血小板减少综合征（severe fever with thrombocytopenia syndrome，SFTS）病毒情况，分离病毒，分析其基因特征。方法 2017年4月~9月，在吉林省图们江流域中朝俄边境地区珲春市、图们市、和龙市、龙井市采集蜱虫进行形态学分类，选择新鲜长角血蜱对其进行分组，采用实时荧光定量多聚核苷酸链式反应方法检测SFTS病毒。对阳性样品，利用绿猴肾细胞进行病毒分离，并扩增S、M、L基因片段，比对同源性，建立系统进化树，分析其基因特征。结果长角血蜱主要分布在图们江下游珲春市和图们市，为该两县优势种，分别达到71.85%和87.62%。在珲春市长角血蜱中分离到一株病毒，命名为YBHC-TICK2-2017/CHINA。该病毒S片段（1 746 bp）、M片段（3 336 bp）、L片段（6 376 bp）基因序列与美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）DNA序列数据库中记录的中国和韩国SFTS病毒分离株基因序列同源性为98.00%~99.00%。系统进化树分析，该病毒株S片段基因序列与中国吉林株（KT890282）分为一簇，M片段和L片段基因序列与中国江苏株（KR230781）分为一簇。结论长角血蜱在图们江下游中朝俄边境地区具有广泛的分布。该研究首次在该地区长角血蜱中分离到SFTS病毒，提示该地区为SFTS防控重点区域。
- SFTS /
- 病毒分离 /
- 传播媒介 /
- 长角血蜱
Abstract: Objective To mastered the distribution characteristics of Haemaphysalis longicornis in the Tumen River basin along the border between Russia, Korea and Northeast China, and understand the status of Haemaphysalis longicornis carrying the virus of fever with thrombocytopenia syndrome (SFTS), then, isolate the virus and analyze its genetic characteristics. Method Ticks were collected from Hunchun, Tumen, Helong and Longjing cities in the Tumen River basin of Jilin Province from April to September, 2017. Haemaphysalis longicornis was selected and grouped. SFTS virus was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Virus isolation was carried out in Vero cells, besides, S, M, L gene segments were amplified. The homology of S, M and L gene segments was compared, phylogenetic tree was established, and their gene characteristics were analyzed. Result Haemaphysalis longicornis mainly distributed in Hunchun and Tumen City in the lower reaches of Tumen River. It was the dominant species in the two counties, reaching 71.85% and 87.62% respectively. A virus named YBHC-TICK2-2017/CHINA was isolated from Haemaphysalis longicornis collected in Hunchun. The sequences of S segment (1 746 bp), M segment (3 336 bp) and L segment (6 376 bp) of the virus were 98.00%-99.00%, homologous to those of SFTS virus isolates from China and Korea recorded in National Center for Biotechnology Information (NCBI) Gen Bank. Phylogenetic tree analysis showed that the S segment gene sequence of the virus strain was divided into a cluster with Jilin strain (KT890282) in China, and M segment and L segment gene sequence with Jiangsu strain (KR230781) in China. Conclusions Haemaphysalis longicornis are widely distributed in the lower reaches of the Tumen river. It was the first time that SFTS virus was isolated from Haemaphysalis longicornis in this area, suggesting that this area is important for SFTS prevention.
- SFTS /
- Virus isolation /
- Vectors /
- Haemaphysalis longicornis

HTML全文

图 1 被YBHC-TICK2-2017/CHINA SFTS病毒株感染的细胞

注：(A)细胞颜色变深，变圆，脱落(×100)；(B)细胞肿胀，胞浆呈颗粒样变化，胞膜边缘不整齐，变圆碎裂(×200)

Figure 1. The cells infected with YBHC-TICK2-2017/CHINA SFTSV

下载: 全尺寸图片幻灯片

图 2 YBHC-TICK2-2017/CHINA SFTSV S基因片段扩增结果

Figure 2. Detection of the YBHC-TICK2-2017/CHINA S Segment gene by RT-PCR

下载: 全尺寸图片幻灯片

图 3 YBHC-TICK2-2017/CHINA SFTSV M基因片段扩增结果

Figure 3. Detection of the YBHC-TICK2-2017/ CHINA M Segment gene by RT-PCR

下载: 全尺寸图片幻灯片

图 4 YBHC-TICK2-2017/CHINA SFTSV L基因片段扩增结果

Figure 4. Detection of the YBHC-TICK2-2017/CHINA L Segment gene by RT-PCR

下载: 全尺寸图片幻灯片

图 5 YBHC-TICK2-2017/CHINA SFTSV L基因片段扩增结果

Figure 5. Detection of the YBHC-TICK2-2017/ CHINA L Segment gene by RT-PCR

下载: 全尺寸图片幻灯片

图 6 YBHC-TICK2-2017/CHINA S片段系统进化分析

Figure 6. Phylogenetic analysis of YBHC-TICK2-2017/CHINA M segment

下载: 全尺寸图片幻灯片

图 7 YBHC-TICK2-2017/CHINA M片段系统进化分析

Figure 7. Phylogenetic analysis of YBHC-TICK2-2017/CHINA M segment

下载: 全尺寸图片幻灯片

图 8 YBHC-TICK2-2017/CHINA L片段系统进化分析

Figure 8. Phylogenetic analysis of YBHC-TICK2-2017/CHINA L segment

下载: 全尺寸图片幻灯片

表 1 不同县蜱构成[n(%)]

Table 1. The tick composition in different counties[n(%)]

地点	森林革蜱	嗜群血蜱	日本血蜱	长角血蜱	全沟硬蜱	其他
龙井市	251(16.98)	899(60.83)	317 (21.45)	0 (0.00)	10 (0.68)	1 (0.07)
和龙市	3(0.45)	5(0.75)	249 (37.39)	0 (0.00)	409 (61.41)	0 (0.00)
珲春市	30(2.76)	15(1.38)	26 (2.39)	781 (71.85)	12 (1.10)	223 (20.52)
图们市	2(0.40)	12(2.40)	41 (8.18)	439 (87.62)	1 (0.20)	6 (1.20)
合计	286(7.66)	931(24.95)	633 (16.96)	1 220 (32.69)	432 (11.58)	230 (6.16)

下载: 导出CSV

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