长沙市活禽市场人H9N2亚型禽流感感染来源的研究

张如胜; 黄政; 刘晓蕾; 姚栋; 陈静芳; 袁洁; 欧新华; 孙边成

doi:10.16462/j.cnki.zhjbkz.2019.05.015

长沙市活禽市场人H9N2亚型禽流感感染来源的研究

doi: 10.16462/j.cnki.zhjbkz.2019.05.015

410007 长沙, 长沙市疾病预防控制中心

基金项目:

湖南省自然科学基金 2018JJ6084

湖南省卫生计生委2017年度科研项目 B2017204

长沙市科技计划项目 kq1701022

详细信息

通讯作者:
孙边成, E-mail: sunbiancheng2013@163.com

中图分类号: R373.13
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出版历程
- 收稿日期: 2018-10-08
- 修回日期: 2019-03-01
- 刊出日期: 2019-05-10

Study on the source of human infection with H9N2 influenza virus from live poultry markets in Changsha

Center for Disease Control and Prevention, Changsha 410007, China

Funds:

The Hunan Provincial Natural Science Foundation 2018JJ6084

The Hunan Provincial Health Medicine Research Project B2017204

Science Foundation of Changsha City kq1701022

More Information

Corresponding author: Sun Bian-cheng, E-mail: sunbiancheng2013@163.com

摘要

摘要: 目的探讨人H9N2亚型禽流感病毒(avian influenza virus，AIV)的感染来源。方法对2014-2015年长沙市活禽市场环境开展AIV核酸监测，收集全球人感染H9N2 AIV病例数据，利用MEGA 6软件对全球人感染H9N2 AIV、活禽市场环境H9N2 AIV和部分禽H9N2 AIV血凝素(hemagglutinin，HA)、神经氨酸酶(neuraminidase，NA)及非结构蛋白(nonstructural protein，NS)基因进行进化分析。结果 2014-2015年长沙市活禽市场环境中H9 AIV的核酸阳性率最高(占44.76%)，污染较为严重。全球共报告27例人感染H9N2 AIV病例，绝大多数病例经治疗后康复，流行病学调查显示59.26%(16/27)的病例明确有禽类或活禽市场暴露史。HA、NA及NS进化分析显示2013-2016年分离自湖南、广东的人源H9N2 AIV分离株与湖南和广东辖区活禽市场环境中分离到的H9N2 AIV亲缘关系近，核苷酸相似性高达97%~99%。结论活禽市场是人H9N2 AIV的感染来源之一。
- 新发传染病 /
- H9N2 /
- 禽流感病毒 /
- 人感染 /
- 来源 /
- 活禽市场
Abstract: Objective To explore the source of human infection H9N2 avian influenza virus (AIV). Methods Environmental AIV nucleic acid monitoring was conducted for live poultry markets in Changsha city from 2014 to 2015, and data of human infection H9N2 subtype AIV cases worldwide were collected. Phylogenetic trees of hemagglutinin(HA), neuraminidase(NA)and non-structural protein(NS)genes from human infection H9N2 subtype AIV, the live poultry markets environmental H9N2 subtype AIV and partial avian H9N2 subtype AIV were constructed using the MEGA 6.06 software, respectively. Results In 2014-2015, H9 subtype AIV had the highest nucleic acid positive rate (44.76%) in the live poultry markets environment of Changsha city, and the pollution was serious. A total of 27 cases of human infection with H9N2 subtype AIV had been reported worldwide, and most of these patients recovered after treatments.Epidemiological survey showed that 59.26% (16/27) of cases had a clear history of exposure to poultry or live poultry markets. The phylogenetic trees of HA, NA and NS genes showed that the human infection H9N2 subtype AIV isolates isolated from Hunan and Guangdong were closely related to the H9N2 subtype AIV isolated from the live poultry markets environment in Hunan and Guangdong provinces from 2013 to 2016. The nucleotide similarity was as high as 97%-99%. Conclusion Live poultry market is one of the sources of human infection with H9N2 influenza virus.
- Emerging infectious diseases /
- H9N2 /
- Avian influenza virus /
- Human infection /
- Source /
- Live poultry market

HTML全文

图 1 H9N2 AIV HA基因进化分析

注：▲为人源H9N2病毒株序列。

Figure 1. Phylogenetic analysis of the HA genes of the influenza H9N2 viruses

下载: 全尺寸图片幻灯片

图 2 H9N2 AIV NA基因进化分析

注：▲为人源H9N2病毒株序列。

Figure 2. Phylogenetic analysis of the NA genes of the influenza H9N2 viruses

下载: 全尺寸图片幻灯片

图 3 H9N2 AIV NS基因进化分析

注：▲为人源H9N2病毒株序列。

Figure 3. Phylogenetic analysis of the NS genes of the influenza H9N2 viruses

下载: 全尺寸图片幻灯片

表 1 2014-2015年长沙市活禽市场环境AIV监测[n(%)]

Table 1. Surveillance of environmental AIV from live poultry markets in Changsha city, China from 2014 to 2015 [n(%)]

样本	数量(个)	A型及亚型阳性标本数及检出率[n(%)]
样本	数量(个)	A	H9	H5	H7	H5, H9	H5, H7	H7, H9	H5, H7, H9	其他
禽类饮水	386	314(81.35)	207(53.63)	111(28.76)	15(3.89)	51(13.21)	7(1.81)	7(1.81)	3(0.78)	22(8.29)
禽类污水	352	223(63.35)	128(25.34)	124(36.36)	9(2.56)	64(18.18)	2(0.57)	7(1.99)	1(0.28)	36(10.23)
笼具涂抹样	35	20(57.14)	11(31.43)	6(17.14)	2(5.71)	2(5.71)	1(2.86)	0(0)	0(0)	2(5.71)
χ²值						21.685
P值						0.001
合计	773	557(72.06)	346(44.76)	241(31.18)	26(3.36)	117(15.14)	10(1.29)	14(1.81)	4(0.52)	60(7.76)

下载: 导出CSV

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