2017-2018年青岛地区儿童急性上呼吸道感染病毒谱与流行特征

张凤; 史晓燕; 赵丹; 杨雯; 孙睿; 苏志磊; 汪照国; 高汝钦

doi:10.16462/j.cnki.zhjbkz.2019.06.015

2017-2018年青岛地区儿童急性上呼吸道感染病毒谱与流行特征

doi: 10.16462/j.cnki.zhjbkz.2019.06.015

266033 青岛, 青岛市疾病预防控制中心(青岛市预防医学研究院)微生物检验科

基金项目:

青岛市民生科技计划项目 17-3-3-2-nsh

详细信息

通讯作者:
高汝钦, E-mail: gaoruqin@sohu.com

中图分类号: R511;R373.1;R441.3
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出版历程
- 收稿日期: 2018-11-30
- 修回日期: 2019-04-11
- 刊出日期: 2019-06-10

Etiology spectrum of virus in acute upper respiratory infection in children from 2017 to 2018 in Qingdao

Department of Pathogenic Microorganism Laboratory, Qingdao Municipal Center for Disease Control and Prevention(Qingdao Institute of Preventive Medicine), Qingdao 266033, China

Funds:

Qingdao People's Livelihood Science and Technology Plan 17-3-3-2-nsh

More Information

Corresponding author: GAO Ru-qin, E-mail: gaoruqin@sohu.com

摘要

摘要: 目的了解2017年3月-2018年3月青岛地区儿童急性上呼吸道感染病毒谱与流行特征。方法随机抽选青岛地区符合国家流感监测方案诊断标准的流感监测病例中的儿童流感样病例（influenza-like illness，ILI）作为研究对象，共检测359例。采集患儿咽拭子，采用多重实时荧光逆转录-聚合酶链反应进行核酸检测的方法，对9种呼吸道病毒进行筛查。结果 359例标本中200例至少有1种病毒检测结果为阳性，阳性率为55.71%（200/359）。其中，阳性最多的是流感病毒B型（Influenza Virus B，IVB）Yamagata亚型29.50%（59/200），其次是肠道病毒（Human Enterovirus，HEV）15.00%（30/200），呼吸道腺病毒（Adenovirus，AdV）13.50%（27/200），呼吸道合胞病毒A型（Respiratory Syncytial Virus A，RSVA）12.50%（25/200），流感病毒A型（Influenza Virus A，IVA）H1N1亚型10.00%（20/200）等。2例3种病毒混合感染，1例4种病毒混合感染。结论 2017年3月-2018年3月9种呼吸道病毒在青岛地区均有流行，IVB Yamagata亚型、HEV、AdV、RSVA亚型、IVA H1N1亚型为主要流行病毒。其中流感病毒、HEV、RSV、偏肺病毒（Human metapneumovirus，HMPV）具有明显的季节性分布。9种病毒在各病毒流行月份存在混合感染。
- 儿童 /
- 急性上呼吸道感染 /
- 呼吸道病毒 /
- 流行特征 /
Abstract: Objective To understand the prevalence and epidemiological characteristics of respiratory viruses in influenza-like illness in children during March 2017 to March 2018 in Qingdao. Methods A random selection of influenza surveillance cases (influenza-like illness, ILI) among children in Qingdao area was selected as the research object, and 359 cases were detected. Nasopharyngeal swabs were collected for multiple-fluorescence real-time reverse transcription-polymerase chain reaction nucleic acid detection to screen 9 kinds of respiratory viruses. Results Among the 359 Cases, 200 cases were positive for at least 1 kinds of viruses, and the positive rate was 55.71%(200/359). Among these 200 cases, the most positive numbers were influenza B Yamagata (IVB Yamagata) 29.50%(59/200), followed by enterovirus 15.00%(30/200), respiratory adenovirus (AdV) 13.50%(27/200), respiratory syncytial virus A (RSVA) 12.5%(25/200), influenza A H1N1(IVA H1N1) 10.00%(20/200), etc. 2 cases were 3 kinds of mixed viruses infected and 1 case was 4 kinds of mixed viruses infected. Conclusions Nine kinds of respiratory viruses are prevalent in Qingdao during March 2017 -March 2018. The main prevalence viruses contain influenza B Yamagata, enterovirus, respiratory adenovirus, respiratory syncytial virus A, influenza A H1N1. There is obvious seasonal distribution of influenza, respiratory syncytial virus, enterovirus, metapneumovirus. A mixed infection exists between 9 kinds of respiratory viruses, and mixed infection occurs in the month of the virus epidemic.
- Children /
- Acute respiratory infection /
- Respiratory virus /
- Pathogenic spectrum /
- Epidemic characteristics

HTML全文

图 1 2017年3月-2018年3月青岛地区ILI病例呼吸道病毒分布情况月度分析

注：图A中不同的颜色块代表对应的病毒在该月所占阳性标本数的百分比，图B中折线代表各月检测标本数目和该月病毒总阳性数.

Figure 1. Monthly distribution of respiratory viruses for ILI cases from March 2017 to March 2018 in Qingdao

下载: 全尺寸图片幻灯片

图 2 2017年3月-2018年3月青岛地区ILI病例呼吸道病毒混合阳性分布情况月度分析

注：图A中不同的颜色块代表对应的混合病毒在该月所占混合阳性病例标本数的百分比，图B中折线代表各月检测混合阳性病例标本数

Figure 2. Monthly distribution of mixing positive respiratory viruses for ILI cases from March 2017 to March 2018 in Qingdao

下载: 全尺寸图片幻灯片

表 1 不同种类阳性呼吸道病毒患儿的年龄分布(岁)

Table 1. Age distribution of varied respiratory viruses positive cases in children(year)

病毒种类	≤2	3~5	6-15	χ²值	P值
IVB
Yamagata	13.605	15.328	23.377	1.262	0.532
Victoria	0.000	0.000	1.299	3.100	0.212
IVA
H1N1	3.401	8.029	5.195	2.924	0.232
H3N2	2.041	3.650	1.299	1.339	0.512
HEV	8.844	13.139	9.091	1.603	0.449
RSV
RSVA	7.483	8.029	5.195	0.622	0.733
RSVB	3.401	2.190	0.000	4.272	0.118
AdV	6.122	8.029	9.091	0.740	0.691
PIV
PIV-1	0.680	1.460	0.000	1.868	0.393
PIV-2	0.000	0.730	0.000	1.937	0.380
PIV-3	2.721	2.920	1.299	0.674	0.714
PIV-4	1.361	0.730	0.000	1.722	0.423
HRV	4.082	2.920	2.597	0.454	0.797
HMPV	1.361	2.920	1.299	1.077	0.584
HBoV	1.361	1.460	0.000	1.936	0.380
HCoV
OC43	3.401	0.000	0.000	8.885	0.012
OC229E/NL63	0.680	0.730	0.000	0.965	0.617
总阳性率(%)	53.430	59.560	53.250	1.315	0.518

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