2013-2018年度滨州市B型流感病毒血凝素和神经氨酸酶基因进化特征分析

张丽芳; 张静; 孙辉; 黄莹; 尹秀升; 韩锋产

doi:10.16462/j.cnki.zhjbkz.2020.02.006

2013-2018年度滨州市B型流感病毒血凝素和神经氨酸酶基因进化特征分析

doi: 10.16462/j.cnki.zhjbkz.2020.02.006

张丽芳^{1, 2},
张静²,
孙辉²,
黄莹²,
尹秀升²,
韩锋产^1, ,

1.
264000 烟台, 滨州医学院生物化学与分子生物学教研室
2.
256600 滨州, 滨州市疾病预防控制中心微生物检验科

详细信息

通讯作者:
韩锋产, E-mail:hanfengchan@gmail.com

中图分类号: R373.1
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出版历程
- 收稿日期: 2019-07-16
- 修回日期: 2019-10-10
- 刊出日期: 2020-02-10

Phylogenetic analysis of influenza B hemagglutinin and neuraminidase gene in Binzhou City from 2013 to 2018

1.
Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264000, China
2.
Microbiological Laboratory, Binzhou Center for Disease Control and Prevention, Binzhou 256600, China

More Information

Corresponding author: HAN Feng-chan, E-mail:hanfengchan@gmail.com

摘要

摘要: 目的分析滨州市2013-2018监测年度B型流感病毒血凝素(hemagglutinin, HA)、神经氨酸酶(neuraminidase, NA)基因突变及其抗原变异特征。方法采集滨州市2013-2018年的流感监测哨点医院流感样病例(influenza-like illness, ILI)咽拭子标本进行病毒分离, 选取29株B型流感分离代表毒株进行HA、NA基因序列测定, 通过基因分析软件进行同源性比对、构建HA、NA基因进化树。结果 2013-2018各年度B型流感占比分别为47.0%、23.0%、31.0%、7.2%和65.1%, 差异有统计学意义(χ²=1 485.143, P < 0.001)。2013-2014年度分离株与当年世界卫生组织推荐的疫苗株匹配度差。2013-2014年度流行的B型流感病毒为Yamagata系HA与Victoria系NA基因的重配株。结论 2013-2018监测年度, 滨州市B型流感病毒的进化是通过系间基因重配、不同谱系共流行、连续不断的不同抗原谱系的更替来完成的。
- B型流感病毒 /
- 血凝素 /
- 神经氨酸酶 /
- 变异 /
- 序列比对
Abstract: Objective To investigate the mutation of hemagglutinin(HA), neuraminidase(NA) gene and amino acid variation of influenza B. Methods Throat swab specimens were taken from patient with influenza-like illness(ILI) and were investigated for the influenza virus identification from 2013 to 2018, and 29 strains of influenza B viruses were selected. HA, NA gene sequencing of the viruses were performed and were aligned using molecular evolutionary genetics analysis software. The phylogenetic tree was constructed. Results During the influenza seasons from 2013 to 2018, the detection rates of influenza B virus were 47.0%, 23.0%, 31.0%, 7.2% and 65.1%, respectively. Statistically significant differences between the proportion of influenza B viruses were observed(χ²=1 485.143, P < 0.001). The isolates from 2013 to 2014 did not correspond to vaccine strain, which was recommended by world health organization for the influenza season. Conclusions The evolution of influenza B virus was achieved through interlineal gene recombination, co-prevalence of different lineages, and continuous replacement of different antigenic lineages in Binzhou from 2013 to 2018.
- Influenza B virus /
- Hemagglutinin /
- Neuraminidase /
- Variation /
- Sequence alignment

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图 1 2013-2018年度滨州市B型流感血凝素基因进化树

注:■代表世界卫生组织推荐的疫苗株, ○代表NA基因发生系间重配的毒株

Figure 1. Phylogenetic tree of HA gene of influenza B virus isolated in Binzhou from 2013 to 2018

下载: 全尺寸图片幻灯片

图 2 2013-2018年度滨州市B型流感神经氨酸酶基因进化树

注:■代表世界卫生组织推荐的疫苗株, ○代表NA基因发生系间重配的毒株

Figure 2. Phylogenetic tree of NA gene of influenza B virus isolated in Binzhou from 2013 to 2018

下载: 全尺寸图片幻灯片

表 1 2013-2018年度滨州B型流感监测情况

Table 1. Influenza surveillance of B in Binzhou from 2013 to 2018

年度	监测标本数(例)	阳性数(例)	阳性率(%, 95% CI)	B型Victoria系	B型Yamagata系
年度	监测标本数(例)	阳性数(例)	阳性率(%, 95% CI)	n(%)	n(%)
2013-2014	903	115	12.7(10.5~14.9)	0(0.0)	54(47.0)
2014-2015	788	61	7.7(5.8~9.6)	0(0.0)	14(23.0)
2015-2016	773	126	16.3(13.7~18.9)	35(27.8)	4(3.2)
2016-2017	1 065	125	11.7(9.7~13.6)	9(7.2)	0(0.0)
2017-2018	1 239	338	27.3(24.8~29.8)	0(0.0)	220(65.1)
合计	4 768	765	16.0(15.0~16.9)	44(5.8)	292(38.2)

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