2013-2016年山东省食品中单核细胞增生李斯特菌的血清型、耐药性及分子分型分析

侯配斌; 陈玉贞; 李心朋; 李文; 刘静; 董海燕; 王梅; 张华宁

doi:10.16462/j.cnki.zhjbkz.2020.02.008

2013-2016年山东省食品中单核细胞增生李斯特菌的血清型、耐药性及分子分型分析

doi: 10.16462/j.cnki.zhjbkz.2020.02.008

250014 济南, 山东省疾病预防控制中心, 山东省传染病预防控制重点实验室

基金项目: 山东省医药卫生科技发展计划项目(2016WS0383, 2018WS307, 2018WS304);山东省自然科学基金培养项目(ZR2015PH059); “十三五”科技重大专项(2018ZX10733402-014)

详细信息

通讯作者:
张华宁, E-mail:huaningzhang@126.com

中图分类号: R181.2
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出版历程
- 收稿日期: 2019-08-03
- 修回日期: 2019-10-25
- 刊出日期: 2020-02-10

Antibiotic resistance, serotype and molecular typing of Listeria monocytogenes from foods in Shandong Province from 2013 to 2016

Shandong Center for Disease Control and Prevention, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Jinan 250014, China

Funds: Shandong Medicine and Health Science Technology Development Project(2016WS0383, 2018WS307, 2018WS304); Shandong Natural Science Foundation for Talent Cultivation(ZR2015PH059); Major Science and Technology Projects(2018ZX10733402-014)

More Information

Corresponding author: ZHANG Hua-ning, E-mail:huaningzhang@126.com

摘要

摘要: 目的了解分离于2013-2016年山东省食品中单核细胞增生李斯特菌(Listeria monocytogenes, LM)的主要血清型、耐药谱和分子分型特点。方法采用微量肉汤稀释法测定LM抗生素敏感性, 使用玻片凝集法和PCR方法确定其血清型, 利用脉冲场凝胶电泳(pulsed field gel electrophoresis, PFGE)和多位点序列分型(multilocus sequence typing, MLST)进行分子分型。结果 191株LM对8种抗生素总体基本敏感, 四环素耐药率最高(15/191, 7.85%), 8种抗生素耐药性连续监测4年无差异(P=1.000);血清型1/2a占38.82%(66/170), 1/2b占18.82%(32/170), 1/2c占42.36%(72/170); SDSRZXDZ016、S2014L031和SDSRZX030带型共占33.78%, 呈明显聚集性, 为优势带型; 优势ST型为ST9、ST8和ST121, 占81.18%(69/85), 临床常见ST3, ST7和ST87占8.23%(7/85), 未发现新ST型。结论 2013-2016年分离于山东省的食源性LM菌株对所测定的大多数抗生素敏感, 但已经发现耐四环素和红霉素菌株; 优势血清型为1/2c和1/2a, 未发现易引起李斯特菌病暴发的4b血清型; SDSRZXDZ016、S2014L031和SDSRZX030为山东省2013-2016年PFGE优势带型; 主要ST型为ST8、ST9和ST121, 临床常见的ST3、ST7和ST87型均从食品中分离到, 应予以重视。
- 单核细胞增生李斯特菌 /
- 耐药性 /
- 血清型 /
- 脉冲场凝胶电泳 /
- 多位点序列分型
Abstract: Objective To understand the main serotypes, antibiotic resistance profiles and molecular typing characteristics of Listeria monocytogenes(LM) isolated from foods in Shandong Province from 2013 to 2016. Methods The antibiotic sensitivity of LM was tested by broth microdilution method. The serotypes were determined by slide agglutination and PCR, and the molecular typing was carried out by pulsed field gel electrophoresis(PFGE) and multilocus sequence typing(MLST). Results Most of 191 LM strains were sensitive to the eight antibiotics tested. Tetracycline resistance was most prevalent(15/191, 7.85%). There was no significant difference in the 8 antibiotic resistance monitored for 4 years(P=1.000). The serotype 1/2 a, 1/2 b and 1/2 c accounted for 38.82%(66/170), 18.82%(32/170), and 42.36%(72/170), respectively. The patterns of SDSRZXDZ016, S2014 L031 and SDSRZX030, totally accounted for 33.78%, were the dominant types. The main ST types were ST9, ST8 and ST121, which accounted for 81.18%(69/85). The clinical common types, ST3, ST7 and ST87 accounted for 8.23%(7/85), mainwhile new ST type was not found. Conclusion The LM strains isolated in Shandong Province from 2013 to 2016 were sensitive to most antibiotics, but some strains were resistant to tetracycline and erythromycin. The dominant serotypes were 1/2 c and 1/2 a. Serotype 4 b, prone to outbreaks of listeriosis, was not found. The main PFGE types were SDSRZXDZ016, S2014 L031 and SDSRZX030, which were continuously found from 2013 to 2016. The main ST types were ST8, ST9 and ST121. The clinical types, ST3, ST7 and ST87 were isolated from food and should be paid seriously attention to.
- Listeria monocytogenes /
- Antibiotic resistance /
- Serotype /
- PFGE /
- MLST

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图 1 2013-2016年山东省食品中分离的LM血清型分布

Figure 1. Serotype distribution of LM strains from foods in Shandong Province from 2013 to 2016

下载: 全尺寸图片幻灯片

图 2 2013-2016年山东省食品中分离的LM菌株PFGE结果

Figure 2. PFGE results of LM strains from foods in Shandong Province from 2013 to 2016

下载: 全尺寸图片幻灯片

图 3 2013-2016年山东省食品中分离的LM菌株MLST聚类分析结果

注:图中圆圈内数字为ST型别。

Figure 3. MLST results of LM strains from foods in Shandong Province from 2013 to 2016

下载: 全尺寸图片幻灯片

表 1 2013-2016年山东省食品中分离的191株LM药敏试验结果

Table 1. Antibiotic resistance results of 191 LM strains from 2013 to 2016

抗生素	敏感度	2013	2014	2015	2016	耐药率(%)
氨苄西林	敏感(株)	5	27	86	73	0.00
	耐药(株)	0	0	0	0
青霉素	敏感(株)	5	27	86	73	0.00
	耐药(株)	0	0	0	0
四环素	敏感(株)	5	27	73	71	7.85
	耐药(株)	0	0	13	2
美罗培南	敏感(株)	5	27	86	73	0.00
	耐药(株)	0	0	0	0
复方新诺明	敏感(株)	5	27	85	73	0.52
	耐药(株)	0	0	1	0
红霉素	敏感(株)	5	27	83	72	2.09
	耐药(株)	0	0	3	1
万古霉素	敏感(株)	5	27	86	73	0.00
	耐药(株)	0	0	0	0
环丙沙星	敏感(株)	5	27	86	72	0.52
	耐药(株)	0	0	0	1

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