四川凉山彝族成人FTO基因多态性与肥胖的关联研究

刘倩倩; 潘利; 万绍平; 依火伍力; 杨芳; 李铮; 王也; 雍正平; 何慧婧; 单广良

doi:10.16462/j.cnki.zhjbkz.2020.08.008

四川凉山彝族成人FTO基因多态性与肥胖的关联研究

doi: 10.16462/j.cnki.zhjbkz.2020.08.008

刘倩倩¹,
潘利¹,
万绍平²,
依火伍力³,
杨芳⁴,
李铮⁴,
王也¹,
雍正平⁵,
何慧婧^1, ,,
单广良^1, ,

1.
100005 北京, 中国医学科学院基础医学研究所, 北京协和医学院基础学院流行病与卫生统计学系
2.
610041 成都, 四川省肿瘤医院, 四川省癌症防治中心, 电子科技大学医学院附属肿瘤医院
3.
615300 凉山, 普格县疾病预防控制中心
4.
615000 凉山, 西昌市疾病预防控制中心
5.
610072 成都, 四川省医学科学院, 四川省人民医院

基金项目:

国家自然科学基金 81273158

中国医学科学院医学与健康科技创新工程 2016-12M-2-004

详细信息

通讯作者:
单广良, E-mail:guangliang_shan@163.com

何慧婧, E-mail:ivygood1985@163.com

中图分类号: R589.2
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出版历程
- 收稿日期: 2020-01-20
- 修回日期: 2020-06-18
- 刊出日期: 2020-08-10

Association of FTO gene with obesity in Yi adults of Sichuan Province, China

1.
Department of Epidemiology and Statistics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing 100005, China
2.
Sichuan Cancer Hospital& Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China
3.
Puge Center for Disease Control and Prevention, Puge 615300, China
4.
Xichang Center for Disease Control and Prevention, Xichang 615000, China
5.
Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital, Chengdu 610072, China

Funds:

National Natural Science Foundation of China 81273158

Medical and Health Science and Technology Innovation Project of Chinese Academy of Medical Sciences 2016-12M-2-004

More Information

Corresponding author: HE Hui-jing,E-mail:yangli8290@hotmail.com; SHAN Guang-liang, E-mail:guangliang_shan@163.com

摘要

摘要: 目的了解四川凉山彝族成人脂肪和肥胖相关基因（fat mass and obesity associated gene，FTO）多态性与肥胖的关联。方法采用病例对照研究方法，病例组为彝族移民流行病学调查中收集的肥胖者[体重指数（body mass index，BMI）≥28 kg/m²]，共322例；对照组为非肥胖非超重者（BMI < 24 kg/m²），按年龄和性别与病例组进行1：2频数匹配，共644例。采用TaqMan实时荧光定量聚合酶链式反应（polymerase chain reaction，PCR）法对FTO基因（rs1121980和rs17817449）进行基因分型。采用非条件Logistic回归分析模型分析两位点多态性与肥胖的关联。结果彝族农民中，加性模型和显性模型下，rs1121980位点与肥胖均存在统计学关联（加性模型：OR=1.45，95% CI：1.01~2.07，P=0.042；显性模型：OR=1.52，95% CI：1.01~2.31，P=0.048）。彝族农民中未见rs17817449与肥胖存在统计学关联（均有P>0.05）。彝族移民中，两位点（rs1121980和rs17817449）与肥胖均未见统计学关联（均有P>0.05）。结论彝族农民FTO rs1121980多态性与成人肥胖存在统计学关联，A等位基因是肥胖的危险因素。彝族移民FTO两位点与成人肥胖均未见关联。
- 彝族 /
- FTO基因 /
- 肥胖 /
- 移民流行病学 /
Abstract: Objective To investigate the association between fat mass and obesity associated (FTO) gene and obesity in Yi adults in Sichuan Province. Methods A case-control study was performed by recruiting 322 obesity cases [body mass index (BMI)>28 kg/m²] from the Yi migrant study conducted in Sichuan Province. 644 non-overweight and non-obese controls were selected as control group (BMI < 24 kg/m²), they were frequency-matched to the cases by age and gender. FTO gene (rs1121980 and rs17817449) were genotyped using the TaqMan method.Unconditional Logistic regression analysis was used to test the association between genetic polymorphisms and obesity. Results The rs1121980 polymorphism was associated with obesity in Yi farmers (OR=1.45, 95% CI: 1.01-2.07, P=0.042, under additive model; OR=1.52, 95% CI: 1.01-2.31, P=0.048, under dominant model). No significant association between rs17817449 polymorphisms and obesity was observed in Yi farmers(all P>0.05). In Yi migrants, we found no significant association between the FTO single nucleotide polymorphism(SNP) and obesity(all P>0.05). Conclusion In Yi farmers, FTO rs1121980 polymorphism was independently associated with adult obesity. In Yi migrants, we found no significant association between the FTO SNPs and adult obesity.
- Yi people /
- FTO gene /
- Obesity /
- BMI /
- Migrant epidemiology

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图 1 SNP分型示意图

Figure 1. Allelic discrimination plot

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表 1 不同移居状态病例组和对照组的基本特征比较[n(%)]

Table 1. The characteristics of cases and controls by migration status[n(%)]

变量	病例组(n=322)			对照组(n=643)			彝族人群		农民		移民
变量	彝族人群	农民(n =126)	移民(n=196)	彝族人群	农民(n=462)	移民(n=181)	χ²值	P值	χ²值	P值	χ²值	P值
性别							0.001	0.987	0.823	0.364	1.850	0.174
男性	107(33.23)	39(30.95)	68(34.69)	214(33.28)	163(35.28)	51(28.18)
女性	215(66.77)	87(69.05)	128(65.31)	429(66.72)	299(64.72)	130(71.82)
年龄(岁)							0.002	1.000	8.462	0.076	0.279	0.991
20~	20(6.21)	12(9.52)	8(4.08)	40(6.22)	31(6.71)	9(4.98)
30~	69(21.43)	32(25.40)	37(18.88)	137(21.31)	101(21.86)	36(19.89)
40~	109(33.85)	51(40.48)	58(29.59)	218(33.90)	166(35.93)	52(28.73)
50~	75(23.29)	25(19.84)	50(25.51)	150(23.33)	104(22.51)	46(25.41)
≥60	49(15.22)	6(4.76)	43(21.94)	98(15.24)	60(12.99)	38(20.99)
教育程度^a							38.485	< 0.001	3.863	0.049	4.575	0.032
小学及以下	214(66.67)	108(85.71)	106(54.36)	541(84.14)	423(91.56)	118(65.19)
初中及以上	107(33.33)	18(14.29)	89(45.64)	102(15.86)	39(8.44)	63(34.81)
吸烟情况							0.784	0.376	0.328	0.567	0.034	0.854
从不吸烟	221(68.63)	85(67.46)	136(69.39)	423(65.79)	299(64.72)	124(68.51)
吸烟	101(31.37)	41(32.54)	60(30.61)	220(34.21)	163(35.28)	57(31.49)
饮酒情况							2.916	0.088	0.006	0.941	4.291	0.038
从不饮酒	184(57.14)	79(62.70)	105(53.57)	404(62.83)	288(62.34)	116(64.09)
饮酒	138(42.86)	47(37.30)	91(46.43)	239(37.17)	174(37.66)	65(35.91)
体力活动							13.712	0.001	10.114	0.006	0.984	0.612
轻	62(19.25)	19(15.08)	43(21.94)	150(23.33)	105(22.73)	45(24.86)
中	234(72.67)	101(80.16)	133(67.86)	474(73.72)	352(76.19)	122(67.40)
重	26(8.08)	6(4.76)	20(10.20)	19(2.95)	5(1.08)	14(7.74)
注：^a教育程度缺失1人。

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表 2 病例组和对照组SNP位点基因型分布和等位基因频率及Hardy-Weinbeng平衡检验

Table 2. Genotype distributions and allele frequencies of FTO SNPs and the test for Hardy-Weinberg equilibrium

SNP	A1, A2	基因型(A2A2/A2A1/A1A1)			MAF (%)			HWE-P值
SNP	A1, A2	病例组	对照组	P值	病例组	对照组	P值	HWE-P值
彝族人群
rs1121980	G, A	13/107/202	21/186/436	0.286	20.65	17.73	0.121	0.978
rs17817449	T, G	12/95/215	18/168/457	0.357	18.48	15.86	0.147	0.865
彝族农民
rs1121980	G, A	6/44/76	11/130/321	0.095	22.22	16.45	0.033	0.879
rs17817449	T, G	6/38/82	10/117/335	0.129	19.84	14.83	0.054	0.998
彝族移民
rs1121980	G, A	7/63/126	10/56/115	0.655	19.64	21.00	0.645	0.663
rs17817449	T, G	6/57/133	8/51/122	0.780	17.60	18.51	0.746	0.675
注：A1：野生型等位基因；A2：突变型等位基因；MAF：低频等位基因频率。

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表 3 彝族成人FTO基因多态性与肥胖的关联

Table 3. Association of FTO SNPs with obesity in Yi adults

遗传模型	病例组	对照组	模型1		模型2
遗传模型	病例组	对照组	OR(95% CI)值	P值	OR(95% CI)值	P值
rs1121980
彝族人群
显性(GG/AA+AG)	202/120	436/207	1.25 (0.95~1.66)	0.116	1.20 (0.89~1.62)	0.227
隐性(AG+GG/AA)	309/13	622/21	1.25 (0.62~2.53)	0.540	1.05 (0.49~2.24)	0.901
加性(GG/AG/AA)	202/107/13	436/186/21	1.21 (0.95~1.54)	0.121	1.15 (0.89~1.49)	0.282
彝族农民
显性(GG/AA+AG)	76/50	321/141	1.51 (1.00~2.28)	0.050	1.52 (1.01~2.31)	0.048
隐性(AG+GG/AA)	120/6	451/11	1.99 (0.71~5.59)	0.192	1.72 (0.59~4.97)	0.320
加性(GG/AG/AA)	76/44/6	321/130/11	1.47 (1.03~2.09)	0.034	1.45 (1.01~2.07)	0.042
彝族移民
显性(GG/AA+AG)	126/70	115/66	0.97 (0.64~1.48)	0.888	0.94 (0.61~1.45)	0.781
隐性(AG+GG/AA)	189/7	171/10	0.62 (0.23~1.67)	0.339	0.60 (0.22~1.66)	0.327
加性(GG/AG/AA)	126/63/7	115/56/10	0.92 (0.65~1.31)	0.643	0.90 (0.63~1.29)	0.556
rs17817449
彝族人群
显性(TT/GG+GT)	215/107	457/186	1.22 (0.92~1.63)	0.170	1.17 (0.86~1.59)	0.333
隐性(GT+TT/GG)	310/12	625/18	1.35 (0.64~2.84)	0.435	1.16 (0.52~2.59)	0.724
加性(TT/GT/GG)	215/95/12	457/168/18	1.20 (0.94~1.54)	0.151	1.14 (0.87~1.48)	0.343
彝族农民
显性(TT/GG+GT)	82/44	335/127	1.43 (0.94~2.19)	0.097	1.43 (0.93~2.20)	0.101
隐性(GT+TT/GG)	120/6	452/10	2.23 (0.78~6.38)	0.136	1.92 (0.65~5.67)	0.240
加性(TT/GT/GG)	82/38/6	335/117/10	1.43 (0.99~2.05)	0.055	1.40 (0.97~2.02)	0.073
彝族移民
显性(TT/GG+GT)	133/63	122/59	0.99 (0.64~1.53)	0.963	0.96 (0.62~1.50)	0.858
隐性(GT+TT/GG)	190/6	173/8	0.68 (0.23~2.02)	0.486	0.64 (0.21~1.95)	0.436
加性(TT/GT/GG)	133/57/6	122/51/8	0.95 (0.66~1.37)	0.780	0.92 (0.63~1.35)	0.674
注：模型1：校正年龄，性别；模型2：校正年龄、性别、吸烟情况、饮酒情况、体力活动和教育程度。

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表 4 彝族农民和移民FTO rs1121980多态性与BMI的关联(加性模型)

Table 4. Association of FTO rs1121980with BMI in Yi farmers and migrants considering the additive model

人群	[n(x±s)]			模型1		模型2
人群	GG	AG	AA	β(95% CI)值	P值	β(95% CI)值	P值
彝族农民	397 (22.33 ± 4.25)	174 (23.02 ± 4.72)	17 (24.10 ± 6.21)	0.75(0.08~1.41)	0.028	0.71(0.06~1.37)	0.033
彝族移民	241 (26.10 ± 5.10)	119 (25.93 ± 4.91)	17 (25.55 ± 4.13)	-0.17(-1.05~0.72)	0.713	-0.18(-1.07~0.71)	0.690
注：模型1：校正年龄、性别；模型2：校正年龄、性别、吸烟情况、饮酒情况、体力活动和教育程度。

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