广州市2010-2019年4型登革热病例流行特征及其病毒E基因

蒋力云; 刘远; 苏文哲; 罗雷; 曹毅敏; 狄飚; 吴新伟; 张周斌

doi:10.16462/j.cnki.zhjbkz.2021.11.022

广州市2010-2019年4型登革热病例流行特征及其病毒E基因

doi: 10.16462/j.cnki.zhjbkz.2021.11.022

510440 广州，广州市疾病预防控制中心病毒免疫部

基金项目:

广州市科技计划 202002030117

广州市卫生健康科技项目 20191A011059

广州市重点实验室基础研究计划 2021BRP004

广州市医学重点学科建设项目 2021-2023-11

详细信息

通讯作者:
蒋力云，E-mail: year800325@163.com

中图分类号: R512.8;R181.3
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出版历程
- 收稿日期: 2021-01-07
- 修回日期: 2021-03-01
- 网络出版日期: 2021-12-04
- 刊出日期: 2021-11-10

Epidemiology of dengue virus serotype 4 cases and E gene analysis of dengue virus in Guangzhou Province from 2010 to 2019

Department of Virology and Immunology, Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China

Funds:

Guangzhou Science and Technology Department Program 202002030117

Bureau of Health of Guangzhou Municipality 20191A011059

Basic Research Project of Key Laboratory of Guangzhou 2021BRP004

Key Project of Medicine Discipline of Guangzhou 2021-2023-11

More Information

Corresponding author: JIANG Li-yun, E-mail: year800325@163.com

摘要

摘要: 目的了解2010-2019年广州市4型登革热病例的流行病学特征和4型登革病毒的分子生物学特征。方法 2010-2019年，收集广州市登革热病例相关资料及血清标本，使用RT-PCR法检测血清标本并分型，对4型登革热病例标本进行登革病毒包膜(envelope, E)蛋白基因序列测定，并进行进化树构建及基因重组分析。结果 2010-2019年，广州市共报告43 174例登革热病例，其中23例为4型登革热病例。4型登革热病例仅分布在5个区，曾在2010年出现本地流行。广州市的4型登革热输入病例主要来自东南亚国家，病毒E基因序列与东南亚国家的序列有较高相似性，病毒基因型归属于印度尼西亚基因型和东南亚基因型，未检测到基因重组。结论应加强对广州市4型登革热病例流行状况和输入病例的监测和关注，并进一步加强对东南亚回国人员登革热常识和个人防护的健康宣传。某些区需要预防出现4型登革病毒的扩散和蔓延。登革病毒基因型转换并未引起登革热在广州市流行强度的变化，有待进一步的观察和研究其是否会发生重组。
- 登革热 /
- 流行特征 /
- E基因 /
- 基因进化树
Abstract: Objective Investigate epidemiology of dengue virus serotype 4 cases, and molecular biological characteristics of dengue virus serotype 4 circulating in Guangzhou from 2010 to 2019. Methods Information and serum samples of dengue patients were collected. Serotypes were confirmed by real-time PCR. Envelope (E) gene of dengue virus serotype 4 was sequenced. Phylogenetic tree was constructed, and recombination was analyzed. Results A total of 43 174 dengue cases were reported in Guangzhou between 2010 and 2019, of which 23 cases were confirmed as serotype 4. Serotype 4 cases can only be detected in 5 districts. An endemic of dengue virus serotype 4 broke out in 2010. The imported cases were mainly from Southeast Asia. Sequences of E gene had a high similarity with sequences from Southeast Asia. Sequences acquired in Guangzhou clustered into Indonesia genotype and Southeast Asia genotype. No recombination has been detected. Conclusions Supervision on dengue virus serotype 4 cases and imported cases should be enforced. Conduct propaganda of knowledge about dengue fever and personal protection should be further done among people coming back from Southeast Asia. Dengue virus serotype 4 must be prevented from spreading, especially in some districts. The genotype shift didn't cause any change of epidemic. Although there was no sign of recombination, further research is needed.
- Dengue fever /
- Epidemiological characteristics /
- Envelope gene /
- Phylogenetic tree

HTML全文

图 1 2010-2019年广州市4型登革病毒E基因进化树

Figure 1. Phylogenetic trees of dengue virus serotype 4 based on E gene sequences from 2010 to 2019 in Guangzhou City

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表 1 2010-2019年广州市4型登革热病例统计与分布

Table 1. The summary and distribution of dengue virus serotype 4 cases from 2010 to 2019 in Guangzhou City

年份	分布区	总病例(例)	本地病例(例)	输入病例(例)	输入病例来源国家/地区
2010	白云区、越秀区、天河区	14	14	0
2012	越秀区	1	1	0
2016	增城区	1	0	1	中国(东莞市)
2017	增城区	1	0	1	缅甸
2018	白云区、天河区	5	2	3	柬埔寨、菲律宾
2019	番禺区	1	0	1	缅甸
注: ▲标注为广州市分离毒株。

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