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摘要:
目的 探讨中国柯萨奇病毒A组2型(coxsackievirus A2, CV-A2)毒株的遗传进化特征。 方法 收集中国CV-A2毒株的VP1区及全基因序列，采用MEGA 10.2.4软件构建系统进化树并开展氨基酸位点突变分析，采用SimPlot 3.5.1软件进行基因重组分析。 结果 共收集到118条VP1序列和26条全基因序列。CV-A2毒株主要来源于广东省，毒株数量自2011年开始迅速增多。基于VP1序列构建的进化树有7个分支，原型毒株为独立A组，多数CV-A2毒株聚集于G组。具有全基因组序列的CV-A2代表株的基因重组分析显示毒株间存在基因重组。另外，VP1氨基酸序列共有22个突变位点。 结论 中国流行的CV-A2毒株已发生较明显的基因重组和VP1区域氨基酸位点突变，监测CV-A2的演化对于手足口病的防控具有重要意义。 Abstract:Objective To analyze the phylogenetic character of coxsackievirus A2 (CV-A2) strains in China. Methods Sequences of the VP1 region and complete genomes of CV-A2 strains in China were collected. The phylogenetic tree and analysis of amino acid mutation were conducted by MEGA 10.2.4 software. The analysis of genetic recombination was carried out through SimPlot 3.5.1 software. Results A total of 118 sequences of CV-A2 VP1 were collected, and 26 strains had complete genome sequences. CV-A2 strains mainly originated from Guangdong Province. The number of CV-A2 strains had increased rapidly since 2011. Phylogenetic tree based on the VP1 region was clustered into seven distinct lineages, and the prototype strains were a separate lineage A while most of strains gathered in lineages G. The analysis of gene recombination of CV-A2 representative strains with complete genome sequences exhibited recombinant segments. The amino acid variations were found at 22 sites of VP1 protein. Conclusion CV-A2 strains circulating in China have undergone gene recombination and mutations in the amino acid sites of the VP1 region, suggesting that monitoring the evolution of CV-A2 is of great significance for the prevention and control of hand-foot-mouth disease. -
Key words:
- Coxsackievirus A2 /
- Systematic evolution /
- Gene recombination
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图 1 基于CV-A2病毒VP1区域核苷酸序列的系统进化树
Figure 1. Phylogenetic tree based on the VP1 region of CV-A2 strains
图 2 基于CV-A2全基因组核苷酸序列的系统进化树
Figure 2. Phylogenetic tree based on the whole genome of CV-A2 strains
图 3 代表株HQ728259.1(A)、JX867330.1(B)与其他HEV-A全基因组的相似性图和BootScan分析
Figure 3. Similarity plot and BootScan analyses based on the whole genome of representative strains HQ728259.1(A), JX867330.1(B) and other HEV-A strains
图 4 代表株KP289359.1(A)、KX156350.1(B)与其他EV-A全基因组的相似性图和BootScan分析
Figure 4. Similarity plot and BootScan analyses based on the whole genome of representative strains KP289359.1(A), KX156350.1(B) and other HEV-A strains
图 5 代表株MF281257.1(A)、MF422535.1(B)与其他HEV-A全基因组的相似性图和BootScan分析
Figure 5. Similarity plot and BootScan analyses based on the whole genome of representative strains MF281257.1(A), MF422535.1(B) and other HEV-A strains
表 1 参考毒株序列
Table 1. Sequences of reference strains
血清型 名称 GenBank序列号 CV-A2 Fleetwood AY421760 CV-A3 Olson AY421761 CV-A4 High Point AY421762 CV-A5 Swatrz AY421763 CV-A6 Gdula AY421764 CV-A7 Parker AY421765 CV-A8 Donovan AY421766 CV-A10 Kowalik AY421767 CV-A12 Texas-12 AY421768 CV-A14 G-14 AY421769 CV-A16 G-10 U05876 EV-A71 BrCr U22521 EV-A76 FRA91-10369 AY697458 EV-A89 BAN00-10359 AY697459 EV-A90 BAN99-10399 AY697460 EV-A91 BAN00-10406 AY697461 EV-A92 USA/GA99-RJg-7 EF667344 
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