老年人群肠道菌群α多样性影响因素研究

殷召雪; 白雪梅; 张晶睿; 姜远静; 闫斐; 木克热木·于努斯; 高欣; 吴静

doi:10.16462/j.cnki.zhjbkz.2022.12.012

老年人群肠道菌群α多样性影响因素研究

doi: 10.16462/j.cnki.zhjbkz.2022.12.012

1.
102206 北京，中国疾病预防控制中心慢病和老龄健康管理处
2.
100069 北京，首都医科大学
3.
118001 丹东, 丹东市元宝区疾病预防控制中心
4.
841000 库尔勒，新疆库尔勒市疾病预防控制中心

基金项目:

公共卫生应急反应机制的运行-慢病防治项目 131031001000210001

科技部重点研发计划项目 2020YFC2003000

详细信息

通讯作者:
殷召雪，E-mail: yinzx@chinacdc.cn

吴静, E-mail: wujing@chinacdc.cn

中图分类号: R117
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- 文章访问数: 340
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- 被引次数: 0
出版历程
- 收稿日期: 2021-11-15
- 修回日期: 2022-06-28
- 网络出版日期: 2022-12-30
- 刊出日期: 2022-12-10

Study on factors influencing gut microbiota α diversity in the older people

1.
Division of non-communicable diseases and elderly health management, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2.
Capital Medical University, Beijing 100069, China
3.
Yuanbao Center for Disease Control and Prevention, Dandong 118001, China
4.
Korla Center for Disease Control and Prevention, Korla 841004, China

Funds:

Operation of the Public Health Emergency Response Mechanism-NCD Prevention and Control Program 131031001000210001

National Key R & D Program of China 2020YFC2003000

More Information

Corresponding author: YIN Zhao-xue, E-mail: yinzx@chinacdc.cn; WU Jing, E-mail: wujing@chinacdc.cn

摘要

摘要: 目的研究老年人肠道菌群α多样性的影响因素。方法对社区老年人肠道菌群16Sr-DNA测序并分析菌群α多样性。按照四分位数将α多样性分为“高”和“低”两组，利用logistic回归分析模型分析肠道菌群α多样性的影响因素。结果多因素logistic回归分析模型分析结果显示，与最低三分位数组相比，精制谷物和水果摄入量最高三分位数组肠道菌群“高”Shannon指数OR(95% CI)值分别为0.57(0.36~0.89)和1.86(1.15~3.02)；较高频次酸菜/泡菜摄入者肠道菌群“高”Shannon指数OR(95% CI)值为0.47(0.27~0.81)。与Shannon指数类似，精制谷物、酸菜/泡菜和水果也是Simpson指数的影响因素，此外，性别也是Simpson指数的影响因素(P=0.028)。结论增加水果摄入有助于增加老年人肠道菌群α多样性，而精制谷物、酸菜/泡菜的摄入则可能不利于α多样性的增加。
- /
- 肠道菌群α多样性 /
- 影响因素 /
- 老年人
Abstract: Objective To explore factors influencing α diversity of gut micro-biota in the older people. Methods Stool samples were collected to extract gut microbiota DNA, 16Sr-DNA sequencing was conducted, and gut microbiota α diversity was analyzed. α diversity was categorized as 'High' or 'Low' according to the quantile of every indicator. Logistic regression models were run to analyze the potential factors that influencing gut microbiota diversity. Results Multivariate logistic regressions showed, compared with the lowest tertile group, OR(95% CI) of high Shannon index in the higher tertile group of refined grain and fruit intake was 0.57(0.37-0.88) and 2.03(1.27-3.24), respectively. Compared with the low group, the possibility of high Shannon index in the high group of pickle intake frequency declined, with OR of 0.45(0.26-0.78). Similarly with Shannon index, refined grain, pickles intake frequency, and fruit intake were all factors influencing the Simpson's index, as well as sex (P=0.028). Conclusions Increasing fruit intake may be beneficial for α diversity of gut microbiota, while intake of refined grain and pickles may be harmful in the older people.
- Gut microbiota /
- α diversity /
- Influencing factors /
- Older people

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表 1 研究对象基本特征与肠道菌群α多样性(x±s)

Table 1. Characterics and gut microbiota α diversity of participants(x±s)

特征	合计	男	女	t/χ²值	P值
例数	648	302	346
年龄[(x±s), 岁]	72.02±4.47	72.10±4.51	71.95±4.43	0.440	0.659
饮酒[n(%)]
≥1次/月	85(13.12)	72(23.84)	13(3.76)	57.072	< 0.001
< 1次/月	563(83.88)	230(76.16)	347(96.24)
体育锻炼[n(%)]
是	468(72.22)	223(73.84)	245(70.81)	0.739	0.390
否	180(27.78)	79(26.16)	101(29.19)
精制谷物摄入量[M(P₂₅, P₇₅), g]	200.00(110.71, 300.00)	200.00(114.29, 300.00)	200.00(107.14, 255.00)	3.322	0.068
新鲜蔬菜摄入量[M(P₂₅, P₇₅), g]	200.00(100.00, 205.00)	200.00(100.00, 300.00)	200.00(100.00, 200.00)	1.193	0.275
酸菜/泡菜摄入频率[n(%)]
≥1次/周	123(18.98)	53(17.55)	70(20.23)	0.754	0.385
< 1次/周	525(81.02)	249(82.45)	276(79.77)
水果摄入量[M(P₂₅, P₇₅), g]	42.86(14.29, 100.00)	42.86(14.28, 100.00)	42.86(14.29, 100.00)	0.285	0.593
红肉摄入量[M(P₂₅, P₇₅), g]	28.57(13.33, 50.00)	28.57(14.29, 57.14)	25.71(11.43, 42.86)	6.482	0.011
牛奶摄入量[M(P₂₅, P₇₅), g]	42.86(0.00, 180.00)	67.85(1.37, 200.00)	28.57(0.00, 114.29)	9.812	< 0.001
酸奶摄入量[M(P₂₅, P₇₅), g]	0.00(0.00, 28.57)	2.00((0.00, 28.57)	0.00(0.00, 28.57)	0.851	0.356
蛋类摄入量[M(P₂₅, P₇₅), g]	50.00(15.00, 55.00)	50.00(17.14, 55.00)	50.00(14.29, 55.00)	1.582	0.208
Shannon指数	5.77±0.76	5.78±0.76	576±0.74	0.370	0.708
Simpson指数	0.93±0.06	0.93±0.07	0.93±0.06	-0.260	0.791
Chao1指数	800.42±244.33	795.40±226.60	804.80±259.00	0.490	0.625
观察菌种数	678.21±197.41	672.00±175.30	683.70±215.00	-0.447	0.453
注：1.年龄和4项α多样性的指标均以(x±s)表示；2.表中食物摄入量呈偏态分布，故利用[M(P₂₅, P₇₅)]表示，男女两组差异检验用秩和检验(Kruskal-Wallis检验)。

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表 2 肠道菌群高α多样性单因素logistic回归分析

Table 2. Univariate logistic regression analysis for gut microbiota high α diversity

特征	高Shannon指数			高Simpson指数			高Chao1指数			高观察菌种数
特征	OR (95% CI)值	Wald值	P值	OR (95% CI)值	Wald值	P值	OR (95% CI)值	Wald值	P值	OR (95% CI)值	Wald值	P值
年龄(岁)
65~＜75	1.00			1.00			1.00			1.00
≥75	0.89(0.59~1.33)	0.311	0.577	1.33(0.91~1.95)	2.136	0.144	0.93(0.62~1.39)	0.126	0.723	1.01(0.68~1.50)	0.003	0.960
性别
男	1.00			1.00			1.00			1.00
女	0.76(0.53~1.08)	2.383	0.123	0.67(0.48~0.96)	4.791	0.029	1.08(0.76~1.55)	0.207	0.649	1.20(0.84~1.72)	0.999	0.318
饮酒
否	1.00			1.00			1.00			1.00
是	1.06(0.63~1.78)	0.041	0.840	1.21(0.73~2.01)	0.562	0.453	0.78(0.45~1.36)	0.759	0.383	0.85(0.49~1.46)	0.365	0.546
运动
否	1.00			1.00			1.00			1.00
是	1.23(0.82~1.86)	1.023	0.312	1.40(0.92~2.09)	2.505	0.114	1.04(0.70~1.55)	0.041	0.840	0.92(0.62~1.37)	0.164	0.686
精制谷物
T1	1.00			1.00			1.00			1.00
T2	0.57(0.32~0.87)	6.562	0.01	0.82(0.54~1.25)	0.862	0.353	0.53(0.34~0.83)	7.589	0.006	0.67(0.43~1.04)	3.165	0.075
T3	0.57(0.37~0.88)	6.421	0.011	0.62(0.40~0.96)	4.537	0.033	0.84(0.56~1.28)	0.631	0.427	1.04(0.68~1.58)	0.028	0.867
新鲜蔬菜
T1	1.00			1.00			1.00			1.00
T2	0.94(0.62~1.43)	0.083	0.774	1.01(0.67~1.53)	0.005	0.945	0.68(0.45~1.05)	3.093	0.079	0.84(0.55~1.30)	0.603	0.438
T3	1.00(0.63~1.58)	0.001	0.993	0.92(0.59~1.46)	0.119	0.729	1.07(0.69~1.68)	0.108	0.742	1.25(0.80~1.95)	0.946	0.331
酸菜泡菜
< 1次/周	1.00			1.00			1.00			1.00
≥1次/周	0.45(0.26~0.78)	8.373	0.004	0.49(0.29~0.82)	7.366	0.007	0.96(0.61~1.52)	0.029	0.863	0.81(0.51~1.30)	0.751	0.386
水果
T1	1.00			1.00			1.00			1.00
T2	1.69(1.06~2.69)	4.853	0.027	1.92(1.21~3.05)	7.674	0.006	1.08(0.70~1.68)	0.133	0.716	1.38(0.88~2.15)	1.973	0.160
T3	2.03(1.27~3.24)	8.844	0.003	2.08(1.30~3.31)	9.432	0.002	1.06(0.67~1.65)	0.055	0.814	1.35(0.86~2.13)	1.654	0.198
红肉
T1	1.00			1.00			1.00			1.00
T2	1.26(0.82~1.95)	1.129	0.288	0.88(0.57~1.35)	0.353	0.553	1.13(0.73~1.74)	0.278	0.598	1.02(0.66~1.58)	0.008	0.928
T3	1.00(0.64~1.57)	0.001	0.989	0.88(0.58~1.36)	0.316	0.574	1.05(0.68~1.64)	0.053	0.817	1.08(0.70~1.67)	0.116	0.738
牛奶
低	1.00			1.00			1.00			1.00
高	1.06(0.74~1.51)	0.101	0.751	1.30(0.91~1.85)	2.146	0.143	1.13(0.79~1.62)	0.463	0.496	1.29(0.90~1.85)	1.973	0.160
酸奶
低	1.00			1.00			1.00			1.00
高	1.36(0.92~2.02)	2.383	0.123	1.41(0.96~2.08)	3.089	0.078	1.21(0.81~1.79)	0.860	0.354	1.26(0.85~1.86)	1.284	0.257
蛋类
T1	1.00			1.00			1.00			1.00
T2	1.27(0.79~2.06)	0.923	0.337	1.22(0.76~1.97)	0.695	0.404	0.92(0.58~1.46)	0.121	0.728	1.15(0.72~1.85)	0.351	0.554
T3	1.60(1.03~2.49)	4.451	0.035	1.51(0.98~2.33)	3.566	0.059	0.88(0.58~1.35)	0.331	0.565	1.21(0.78~1.86)	0.736	0.391
注：T1、T2、T3分别为食物摄入量最低三分位数组、中间三分位数组和最高三分位数组。

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表 3 肠道菌群高α多样性多因素logistic回归分析

Table 3. Multiple logistic regression analysis for gut microbiota high α diversity

特征	高Shannon指数			高Simpson指数			高Chao1指数			高观察菌种数
特征	OR (95% CI)值	Wald值	P值	OR (95% CI)值	Wald值	P值	OR (95% CI)值	Wald值	P值	OR (95% CI)值	Wald值	P值
性别
男	1.00			1.00			1.00			1.00
女	0.71(0.48~1.05)	2.478	0.116	0.68(0.46~0.99)	4.833	0.028	1.06(0.72~1.55)	0.130	0.718	1.22(0.85~1.76)	1.182	0.276
精制谷物
T1	1.00			1.00			1.00			1.00
T2	0.56(0.36~0.88)	6.215	0.013	0.85(0.55~1.32)	0.522	0.470	0.53(0.34~0.84)	7.200	0.007	0.67(0.43~1.07)	2.840	0.092
T3	0.57(0.36~0.89)	6.058	0.014	0.62(0.40~0.98)	4.259	0.039	0.86(0.56~1.32)	0.471	0.492	1.07(0.70~1.65)	0.106	0.744
酸菜泡菜
< 1次/周	1.00			1.00			1.00			1.00
≥1次/周	0.47(0.27~0.81)	7.424	0.006	0.50(0.29~0.84)	6.836	0.009	0.98(0.62~1.55)	0.011	0.916	0.81(0.50~1.30)	0.786	0.375
水果
T1	1.00			1.00			1.00			1.00
T2	1.63(1.01~2.65)	3.869	0.048	1.98(1.23~3.22)	7.807	0.005	1.03(0.65~1.61)	0.011	0.917	1.32(0.83~2.10)	1.398	0.237
T3	1.86(1.15~3.02)	6.406	0.011	2.01(1.24~3.25)	8.113	0.004	1.03(0.65~1.63)	0.011	0.917	1.27(0.79~2.03)	1.003	0.316
蛋类
T1	1.00			1.00			1.00			1.00
T2	1.19(0.71~1.98)	0.440	0.507	1.12(0.68~1.84)	0.185	0.667	0.89(0.55~1.45)	0.210	0.647	1.06(0.65~1.74)	0.061	0.806
T3	1.55(0.98~2.46)	3.478	0.062	1.38(0.88~2.16)	1.961	0.161	0.96(0.62~1.49)	0.038	0.846	1.25(0.80~1.96)	0.964	0.326
注：T1、T2、T3分别为食物摄入量最低三分位数组、中间三分位数组和最高三分位数组。

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参考文献(10)

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特征	合计	男	女	t/χ²值	P值
例数	648	302	346
年龄[(x±s), 岁]	72.02±4.47	72.10±4.51	71.95±4.43	0.440	0.659
饮酒[n(%)]
≥1次/月	85(13.12)	72(23.84)	13(3.76)	57.072	< 0.001
< 1次/月	563(83.88)	230(76.16)	347(96.24)
体育锻炼[n(%)]
是	468(72.22)	223(73.84)	245(70.81)	0.739	0.390
否	180(27.78)	79(26.16)	101(29.19)
精制谷物摄入量[M(P₂₅, P₇₅), g]	200.00(110.71, 300.00)	200.00(114.29, 300.00)	200.00(107.14, 255.00)	3.322	0.068
新鲜蔬菜摄入量[M(P₂₅, P₇₅), g]	200.00(100.00, 205.00)	200.00(100.00, 300.00)	200.00(100.00, 200.00)	1.193	0.275
酸菜/泡菜摄入频率[n(%)]
≥1次/周	123(18.98)	53(17.55)	70(20.23)	0.754	0.385
< 1次/周	525(81.02)	249(82.45)	276(79.77)
水果摄入量[M(P₂₅, P₇₅), g]	42.86(14.29, 100.00)	42.86(14.28, 100.00)	42.86(14.29, 100.00)	0.285	0.593
红肉摄入量[M(P₂₅, P₇₅), g]	28.57(13.33, 50.00)	28.57(14.29, 57.14)	25.71(11.43, 42.86)	6.482	0.011
牛奶摄入量[M(P₂₅, P₇₅), g]	42.86(0.00, 180.00)	67.85(1.37, 200.00)	28.57(0.00, 114.29)	9.812	< 0.001
酸奶摄入量[M(P₂₅, P₇₅), g]	0.00(0.00, 28.57)	2.00((0.00, 28.57)	0.00(0.00, 28.57)	0.851	0.356
蛋类摄入量[M(P₂₅, P₇₅), g]	50.00(15.00, 55.00)	50.00(17.14, 55.00)	50.00(14.29, 55.00)	1.582	0.208
Shannon指数	5.77±0.76	5.78±0.76	576±0.74	0.370	0.708
Simpson指数	0.93±0.06	0.93±0.07	0.93±0.06	-0.260	0.791
Chao1指数	800.42±244.33	795.40±226.60	804.80±259.00	0.490	0.625
观察菌种数	678.21±197.41	672.00±175.30	683.70±215.00	-0.447	0.453
注：1.年龄和4项α多样性的指标均以(x±s)表示；2.表中食物摄入量呈偏态分布，故利用[M(P₂₅, P₇₅)]表示，男女两组差异检验用秩和检验(Kruskal-Wallis检验)。

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老年人群肠道菌群α多样性影响因素研究

doi: 10.16462/j.cnki.zhjbkz.2022.12.012

通讯作者:
殷召雪，E-mail: yinzx@chinacdc.cn

吴静, E-mail: wujing@chinacdc.cn

计量

Study on factors influencing gut microbiota α diversity in the older people

Corresponding author: YIN Zhao-xue, E-mail: yinzx@chinacdc.cn; WU Jing, E-mail: wujing@chinacdc.cn

计量

目录

留言板

老年人群肠道菌群α多样性影响因素研究

doi: 10.16462/j.cnki.zhjbkz.2022.12.012

通讯作者: 殷召雪，E-mail: yinzx@chinacdc.cn 吴静, E-mail: wujing@chinacdc.cn

计量

出版历程

Study on factors influencing gut microbiota α diversity in the older people

Corresponding author: YIN Zhao-xue, E-mail: yinzx@chinacdc.cn; WU Jing, E-mail: wujing@chinacdc.cn

计量

出版历程

目录

通讯作者:
殷召雪，E-mail: yinzx@chinacdc.cn

吴静, E-mail: wujing@chinacdc.cn