Expression and clinical significance of <i>PLOD</i>3 in gastric adenocarcinoma, an analysis from TCGA data bank

CHEN Qiang; ZHANG Tao; WANG Zi-ran; WU Zheng-sheng; DU Wei-dong

doi:10.16462/j.cnki.zhjbkz.2021.10.022

Volume 25 Issue 10

Nov. 2021

Turn off MathJax

Article Contents

Article Navigation > CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION > 2021 > 25(10): 1235-1240

CHEN Qiang, ZHANG Tao, WANG Zi-ran, WU Zheng-sheng, DU Wei-dong. Expression and clinical significance of PLOD3 in gastric adenocarcinoma, an analysis from TCGA data bank[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(10): 1235-1240. doi: 10.16462/j.cnki.zhjbkz.2021.10.022

Citation:

CHEN Qiang, ZHANG Tao, WANG Zi-ran, WU Zheng-sheng, DU Wei-dong. Expression and clinical significance of PLOD3 in gastric adenocarcinoma, an analysis from TCGA data bank[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2021, 25(10): 1235-1240. doi: 10.16462/j.cnki.zhjbkz.2021.10.022

Citation:

PDF( 22123 KB)

Expression and clinical significance of PLOD3 in gastric adenocarcinoma, an analysis from TCGA data bank

doi: 10.16462/j.cnki.zhjbkz.2021.10.022

1.
Department of Pathology, Anhui Medical University, Hefei 230032, China
2.
Department of Pathology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
3.
Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100055, China

Funds:

The National Natural Science Foundation of China 81472493

The National Natural Science Foundation of China 81972472

The Scientific Research Foundation of the Institute for Translational Medicine of Anhui Province 2017zhyx37

More Information

Corresponding author: DU Wei-dong, E-mail: weidong.du@ahmu.edu.cn
Received Date: 2020-08-31
Rev Recd Date: 2021-04-30

Available Online: 2021-11-17

Publish Date: 2021-10-10

Abstract

Abstract

Objective To analyze the expression of procollagen-lysine, 2-oxoglutarate 5-dioxygenase (PLOD3) and its clinical significance in gastric adenocarcinoma by means of the cancer genome atlas (TCGA) database. Methods The UALCAN database (http://ualcan.path.uab.edu/) was used to analyze differentiated expression of PLOD3 gene in gastric adenocarcinoma tissues and normal gastric tissues in TCGA database. Kaplan-Meier model was designed to analyze the correlation between the prognosis of patients with gastric adenocarcinoma and the expression of PLOD3. LinkedOmics database was implemented to analyze the genes related to PLOD3. Immunohistochemistry was carried out to verify the expression difference of PLOD3 in gastric adenocarcinoma and adjacent normal gastric tissues. Results The database analysis revealed that the expression level of PLOD3 gene in gastric cancer tissues was higher than that in normal gastric tissues (P < 0.001). Expression of PLOD3 gene in gastric adenocarcinoma with p53 mutation was higher than the cases without p53 mutation (P < 0.001). PLOD3 gene expression was significantly correlated with the survival of patients with gastric adenocarcinoma (HR=1.360, 95% CI: 1.150-1.610, P < 0.001). PLOD3 expression was positively correlated with the genes, such as AP1S1, LRWD1, and SLC12A9 (all P < 0.001), and negatively correlated with the genes, such as CGGBP1, ZNF561 and MIER1 (all P < 0.001). The immunohistochemical observation further demonstrated that more PLOD3 protein was expressed in gastric adenocarcinoma tissues than the adjacent noncancerous gastric tissues (P=0.031). Conclusion PLOD3 would become a valuable biomarker for evaluating the prognosis and targeted therapy for patients with gastric adenocarcinoma.
- PLOD3,
- Gene expression,
- Gastric adenocarcinoma,
- Database

FullText(HTML)

References(12)

References

[1]	Sung H, Ferlay J, Siegel RL, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin, 2021, 71(3): 209-249. DOI: 10.3322/caac.21660.
[2]	Guo J, Yu W, Su H, et al. Genomic landscape of gastric cancer: molecular classification and potential targets [J]. Sci China Life Sci, 2017, 60(2): 126-137. DOI: 10.1007/s11427-016-0034-1.
[3]	Oki E, Zhao Y, Yoshida R, et al. The difference in p53 mutations between cancers of the upper and lower gastrointestinal tract [J]. Digestion, 2009, 79(Suppl 1): 33-39. DOI: 10.1159/000167864.
[4]	Qi YF, Xu R. Roles of PLODs in collagen synthesis and cancer progression [J]. Front Cell Dev Biol, 2018, 6: 66. DOI: 10.3389/fcell.2018.00066.
[5]	Nicastri A, Gaspari M, Sacco R, et al. N-glycoprotein analysis discovers new up-regulated glycoproteins in colorectal cancer tissue [J]. J Proteome Res, 2014, 13(11): 4932-4941. DOI: 10.1021/pr500647y.
[6]	Shen QY, Eun JW, Lee K, et al. Barrier to autointegration factor 1, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3, and splicing factor 3b subunit 4 as early-stage cancer decision markers and drivers of hepatocellular carcinoma [J]. Hepatology, 2018, 67(4): 1360-1377. DOI: 10.1002/hep.29606.
[7]	Baek JH, Yun HS, Kwon GT, et al. PLOD3 promotes lung metastasis via regulation of STAT3 [J]. Cell Death Dis, 2018, 9(12): 1138. DOI: 10.1038/s41419-018-1186-5.
[8]	Tsai CK, Huang LC, Tsai WC, et al. Overexpression of PLOD3 promotes tumor progression and poor prognosis in gliomas [J]. Oncotarget, 2018, 9(21): 15705-15720. DOI: 10.18632/oncotarget.24594.
[9]	Wang B, Xu LY, Ge YG, et al. PLOD3 is upregulated in gastric cancer and correlated with clinicopathologic characteristics [J]. Clin Lab, 2019, 65(1): 25-30. DOI: 10.7754/Clin.Lab.2018.180541.
[10]	张显萍, 李莹, 袁才佳, 等. 基于TCGA数据初步筛选胃癌预后相关基因[J]. 临床检验杂志, 2019, 37(5): 396-400. DOI: 10.13602/j.cnki.jcls.2019.05.18. Zhang XP, Li Y, Yuan CJ, et al. Preliminary screening of prognosis-related genes in gastric cancer based on TCGA database [J]. Chin J Clin Lab Sci, 2019, 37(5): 396-400. DOI: 10.13602/j.cnki.jcls.2019.05.18.
[11]	王杨燕, 汪自然, 马筱玲. 基于TCGA数据库分析ALAS1在肝癌中的表达及临床意义[J]. 临床检验杂志, 2020, 38(3): 230-234. DOI: 10.13602/j.cnki.jcls.2020.03.18. Wang YY, Wang ZR, Ma XL. Analysis of the expression and clinical significance of ALAS1 in liver cancer based on TCGA database [J]. Chin J Clin Lab Sci, 2020, 38(3): 230-234. DOI: 10.13602/j.cnki.jcls.2020.03.18.
[12]	Xie D, Li J, Wei S, et al. Knockdown of PLOD3 suppresses the malignant progression of renal cell carcinoma via reducing TWIST1 expression [J]. Mol Cell Probes, 2020, 53: 101608. DOI: 10.1016/j.mcp.2020.101608.